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Front. Microbiol. | doi: 10.3389/fmicb.2019.02703

Unaltered Fungal Burden and Lethality in Human CEACAM1-Transgenic Mice during Candida albicans Dissemination and Systemic Infection

 Esther Klaile1*,  Mario M. Müller1, Saskia Brehme1,  Cristina Zubiria-Barrera1,  Tilman E. Klassert1,  Magdalena Stock1, Adrian Durotin1, Tien D. Nguyen1, Sabina Feer1,  Bernhard B. Singer2,  Peter F. Zipfel3, 4, Sven Rudolphi4, 5, 6,  Ilse D. Jacobsen4, 5, 6 and  Hortense Slevogt1
  • 1ZIK Septomics, University Hospital Jena, Germany
  • 2Institute of Anatomy, Medical Faculty, University of Duisburg-Essen, Germany
  • 3Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Germany
  • 4Institute of Microbiology, Friedrich Schiller University Jena, Germany
  • 5Microbial Immunology, Leibniz Institute for Natural Product Research and Infection Biology, Germany
  • 6Center for Sepsis Control and Care (CSCC), University Hospital Jena, Germany

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, CD66a) is a receptor for Candida albicans. It is crucial for the immune response of intestinal epithelial cells to this opportunistic pathogen. Moreover, CEACAM1 is of importance for the mucosal colonization by different bacterial pathogens. We therefore studied the influence of the human CEACAM1 receptor in human CEACAM1-transgenic mice on the C. albicans colonization and infection utilizing a colonization/dissemination and a systemic infection mouse model. Our results showed no alterations in the host response between the transgenic mice and wild type littermates to the C. albicans infections. Both mouse strains showed comparable C. albicans colonization and mycobiota, similar fungal burdens in various organs, and a similar survival in the systemic infection model. Interestingly, some of the mice treated with anti-bacterial antibiotics (to prepare them for C. albicans colonization via oral infection) also showed a strong reduction in endogenous fungi instead of the normally observed increase in fungal numbers. This was independent of the expression of human CEACAM1. In the systemic infection model, the human CEACAM1 expression was differentially regulated in the kidneys and livers of Candida-infected transgenic mice. Notably, in the kidneys a total loss of the largest human CEACAM1 isoform was observed. However, the overwhelming immune response induced in the systemic infection model likely covered any CEACAM1-specific effects in the transgenic animals. In vitro studies using bone marrow-derived neutrophils from both mouse strains also revealed no differences in their reaction to C. albicans. In conclusion, in contrast to bacterial pathogens interacting with CEACAM1 on different mucosal surfaces, the human CEACAM1-transgenic mice did not reveal a role of human CEACAM1 in the in vivo candidiasis models used here. Further studies and different approaches will be needed to reveal a putative role of CEACAM1 in the host response to C. albicans.

Keywords: Carcinoembryonic antigen-related cell adhesion molecule 1 (CD66a), Candida albicans, colonization, dissemination, mouse model, bone marrow-derived neutrophils, ITS sequencing, Mycobiota

Received: 26 Jun 2019; Accepted: 07 Nov 2019.

Copyright: © 2019 Klaile, Müller, Brehme, Zubiria-Barrera, Klassert, Stock, Durotin, Nguyen, Feer, Singer, Zipfel, Rudolphi, Jacobsen and Slevogt. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Esther Klaile, University Hospital Jena, ZIK Septomics, Jena, Germany, esther.klaile@med.uni-jena.de