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Front. Microbiol. | doi: 10.3389/fmicb.2019.02716

Involvement of Exogenous N-acyl-homoserine Lactones in Spoilage Potential of Pseudomonas fluorescens Isolated from Refrigerated Turbot

Tingting Li1,  Dangfeng Wang2, Haitao Chen3, Likun Ren2,  Yongchao Mei2, Ting Ding4, Qiuying Li2 and  Jianrong Li2*
  • 1Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Minzu University, China
  • 2Bohai University, China
  • 3Beijing Technology and Business University, China
  • 4Jiangnan University, China

Some bacteria can modulate their spoilage potential by responding to environmental signaling molecules via the quorum sensing (QS) system. However, the ability of P. fluorescens, the specific spoilage organism (SSO) of turbot, to response to environmental signaling molecules remains unclear. This study investigated the effects of six synthetic N-acyl homoserine lactones (AHLs) on typical behaviors mediated by QS in P. fluorescens, such as biofilm formation and extracellular protease activity. Total volatile basic nitrogen (TVB-N) was used as a spoilage indicator to evaluate quality changes in AHL-treated turbot fillets during storage. The results confirm the enhancing effect of environmental AHLs on QS-dependent factors of P. fluorescens and quality deterioration of turbot fillets, with C 4 -HSL and C 14 -HSL being the most effective. Moreover, the content decrease of exogenous AHLs was also validated by GC-MS analysis. Further, changes in rhlR transcription levels in P. fluorescens suggest this bacterium can sense environmental AHLs. Finally, molecular docking analysis demonstrates the potential interactions of RhlR protein with various exogenous AHLs. These findings strongly implicate environmental AHLs in turbot spoilage caused by P. fluorescens, suggesting preservation of turbot should not exclusively consider the elimination of SSO-secreted AHLs.

Keywords: Molecule docking, eavesdropping, Pseudomonas fluorescens, Quorum Sensing, Spoilage potential

Received: 04 Jul 2019; Accepted: 08 Nov 2019.

Copyright: © 2019 Li, Wang, Chen, Ren, Mei, Ding, Li and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Jianrong Li, Bohai University, Jinzhou, China,