%A Gupta,Amit Kumar
%A Khan,Md. Shoaib
%A Choudhury,Shubham
%A Mukhopadhyay,Adhip
%A Sakshi,
%A Rastogi,Amber
%A Thakur,Anamika
%A Kumari,Pallawi
%A Kaur,Manmeet
%A Shalu,
%A Saini,Chanchal
%A Sapehia,Vandna
%A Barkha,
%A Patel,Pradeep Kumar
%A Bhamare,Kailash T.
%A Kumar,Manoj
%D 2020
%J Frontiers in Microbiology
%C
%F
%G English
%K SARS-CoV-2,2019-nCoV,COVID-19,Epitopes,Therapeutics,primers
%Q
%R 10.3389/fmicb.2020.01858
%W
%L
%M
%P
%7
%8 2020-July-31
%9 Original Research
%#
%! CoronaVR- Computational resource for SARS-CoV-2 epitopes and therapeutics
%*
%<
%T CoronaVR: A Computational Resource and Analysis of Epitopes and Therapeutics for Severe Acute Respiratory Syndrome Coronavirus-2
%U https://www.frontiersin.org/articles/10.3389/fmicb.2020.01858
%V 11
%0 JOURNAL ARTICLE
%@ 1664-302X
%X In December 2019, the Chinese city of Wuhan was the center of origin of a pneumonia-like disease outbreak with an unknown causative pathogen. The CDC, China, managed to track the source of infection to a novel coronavirus (2019-nCoV; SARS-CoV-2) that shares approximately 79.6% of its genome with SARS-CoV. The World Health Organization (WHO) initially declared COVID-19 as a Public Health Emergency of International Concern (PHEIC) and later characterized it as a global pandemic on March 11, 2020. Due to the novel nature of this virus, there is an urgent need for vaccines and therapeutics to control the spread of SARS-CoV-2 and its associated disease, COVID-19. Global efforts are underway to circumvent its further spread and treat COVID-19 patients through experimental vaccine formulations and therapeutic interventions, respectively. In the absence of any effective therapeutics, we have devised h bioinformatics-based approaches to accelerate global efforts in the fight against SARS-CoV-2 and to assist researchers in the initial phase of vaccine and therapeutics development. In this study, we have performed comprehensive meta-analyses and developed an integrative resource, “CoronaVR” (http://bioinfo.imtech.res.in/manojk/coronavr/). Predominantly, we identified potential epitope-based vaccine candidates, siRNA-based therapeutic regimens, and diagnostic primers. The resource is categorized into the main sections “Genomes,” “Epitopes,” “Therapeutics,” and Primers.” The genome section harbors different components, viz, genomes, a genome browser, phylogenetic analysis, codon usage, glycosylation sites, and structural analysis. Under the umbrella of epitopes, sub-divisions, namely cross-protective epitopes, B-cell (linear/discontinuous), T-cell (CD4+/CD8+), CTL, and MHC binders, are presented. The therapeutics section has different sub-sections like siRNA, miRNAs, and sgRNAs. Further, experimentally confirmed and designed diagnostic primers are earmarked in the primers section. Our study provided a set of shortlisted B-cell and T-cell (CD4+ and CD8+) epitopes that can be experimentally tested for their incorporation in vaccine formulations. The list of selected primers can be used in testing kits to identify SARS-CoV-2, while the recommended siRNAs, sgRNAs, and miRNAs can be used in therapeutic regimens. We foresee that this resource will help in advancing the research against coronaviruses.