Debarati Paul
Anju Arora
Madan L. Verma- 1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India
- 2Division of Microbiology, Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, India
- 3Department of Biotechnology, School of Basic Sciences, Indian Institute of Information Technology, Una, India
Editorial on the Research Topic
Advances in Microbial Biofuel Production
In the recent times, there has been considerable focus on transition to renewable energy sources and on carbon capture and storage (CCS) of CO2. Hence biomass derived alternative energy products and chemicals have gained attention worldwide. The area of biofuel research is very widespread and is evolving rapidly in various aspects in order to meet ever-expanding automobile industries. Various advanced biofuels such as aviation fuels, syngas, biohydrogen, are being implemented recently and novel concepts are being executed for in-depth study/engineering of metabolic pathways or for discovery of novel bioprocesses. Recently biofuel research is geared up to establish the sustainable and economical biorefineries via concomitant production of biofuels, and value added biochemicals such as pigments/nutraceuticals (Singh et al., 2016). Environmental concerns on waste management may be ameliorated by utilizing the massive agricultural and industrial waste for the cultivation of hyperproducer oleaginous microorganisms that grow on a wide variety of sugars derived from waste hydrolysates (Singh et al., 2018; Sinha S. et al., 2020; Sinha et al., 2021). There has been a keen interest in applying versatile microbes for generation of renewable energy fuels from the biomass and biological wastes, not only because of the versatile nature of microbes for producing biofuels and other valuable products, but also due to their ability to use waste as their source of energy and nutrition (Kumar and Kumar, 2017). Using microbes also saves arable land which can otherwise be used for food and fodder and microbial cultivation does not depend upon weather conditions, unlike plant resources.
Advances in microbial biofuel production has been recently achieved through the use of cost-effective innovative feedstock, robust microbes, and stable bio/ nano-catalyst systems (Verma et al., 2016; Arora et al., 2020; Singh G. et al., 2020; Singh N. et al., 2020; Sinha S. et al., 2020). Now-a-days, the bioenergy sector is not restricted to fuel production, but extends to biorefinery where other value-added products using waste/underutilized biomass are generated in order to make the overall process cost-effective (Figure 1). Researchers are now addressing the fundamental issues of escalating fuel prices and depletion of fossil fuels through implementation of cost-effective bioenergy strategies (Sharma et al., 2018a,b, 2021).
Figure 1. The figure showing an overview of advances made in microbial biofuel production.
Developing a robust methodology is a prerequisite requirement for quickly determining the suitability of any novel microbial strain or improvised strain toward bioenergy production (Sharma et al., 2021). For biodiesel production, oleaginous yeast strains are being explored and improved techniques are being employed for screening such strains. The researchers have investigated the insights of stability of fluorescence dye namely Nile red for estimation of lipid content in oleaginous organisms (Ramírez-Castrillón et al.) and demonstrated the occurrence of optimal fluorescence stability period between 20 and 30 min that need to be optimized with regard to chosen strain and reaction conditions. They have further reported that relative fluorescence units can be measured after the 30 min.
The cost-effective feedstock is the ideal requirement for the successful operation of any biofuel industry (Verma et al., 2016). However, the degree of ease and copious abundance of any inexpensive feedstock depends on the specific requirements of a particular geographical region. For example, Mhlongo et al. explored the potential contribution of filamentous fungi toward single cell oils for biodiesel production. However, researchers have emphasized on specific issues that restrict their potential applications and discussed the other routes for synthesis of certain biofuel/biochemicals in order to meet the requirement of ideal filamentous fungi. Another group of researchers explored the lignocellulosic biomass as the alternative feedstock for growing and harvesting of oleaginous microorganisms with the final target of biodiesel production (Chintagunta et al.). Researchers also highlighted the disadvantage associated with conventional feedstock, that should eventually be minimized through the usage of innovative lignocellulosic-based technologies or via cultivating oleaginous microbes for lipid production, detailing the steps of biodiesel production step-wise starting from lignocellulosic biomass to the actual biofuel in purified form. Advantages of cultivating oleaginous microbes for lipid production and challenges in subsequent extraction, processing via transesterification protocols and purification of biodiesel has been discussed.
Rajan et al. developed the novel approach for the prebiotic development for the production of probiotics using the underutilized hemicellulosic feedstocks. They utilized hemicellulosic hydrolysates isolated from plants such as pine, poplar and switchgrass as substrates for culturing prebiotics. The study not only revealed that prebiotic ingredients may be produced from various lignocellulosic biomass, but also correlated the viability and differential growth ability of probiotic microbes to the composition of hemicellulose-based resources.
Thus, the scope of microbial biofuel production is multifaceted that demonstrates the vastness of research and development of the bioenergy research. It may be concluded with the discussion that implementation of the innovative approaches in the design and development of cost-effective feedstocks will help to lower the escalating fuel price and ameliorate the current environmental issues across the globe in the bioenergy sector. Integrating liquid biofuel production to lignocellulosic biomass waste management has gained significant attention from circular economical aspects and environmental benefits. Adoption of such integrated processes would lead to cost-effective technologies that would generate valuable by-product(s) on one hand and regulate anthropogenic emissions of CO2 on the other.
Author Contributions
All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.
Funding
This study was supported by Himachal Pradesh Council for Science, Technology & Environment (HIMCOSTE), Grant Number: STC/F(8)-6/2019(R&D 2019-20)-377 to MV.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
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Acknowledgments
The authors acknowledge their respective institutes or department for support.
References
Arora, A., Nandal, P., Singh, J., and Verma, M. L. (2020). Nanobiotechnological advancements in lignocellulosic biomass pretreatment. Mater. Sci. Energy Technol 3, 308–318. doi: 10.1016/j.mset.2019.12.003
Kumar, R., and Kumar, P. (2017). Future microbial applications for bioenergy production: a perspective. Front. Microbiol. 8:450. doi: 10.3389/fmicb.2017.00450
Sharma, S., Arora, A., Sharma, P., Singh, S., Nain, L., and Paul, D. (2018a). Notable mixed substrate fermentation by native Kodamaea ohmeri strains isolated from Lagenaria siceraria flowers and ethanol production on paddy straw hydrolysates. Chem. Cent. J. 12:18. doi: 10.1186/s13065-018-0375-8
Sharma, S., Ghoshal, C., Arora, A., Samar, W., Nain, L., and Paul, D. (2021). Strain improvement of native Saccharomyces cerevisiae LN ITCC 8246 strain through protoplast fusion to enhance its xylose uptake. Appl. Biochem. Biotechnol. 193, 2455–2469. doi: 10.1007/s12010-021-03539-3
Sharma, S., Vargese, E., Arora, A., Singh, K. N., Singh, S., Nain, L., et al. (2018b). Augmenting pentose utilization and ethanol production of native Saccharomyces cerevisiae LN using medium engineering and response surface methodology. Front. Bioeng. Biotechnol. 6:168. doi: 10.3389/fbioe.2018.00168
Singh, G., Jawed, A., Paul, D., Bandyopadhyay, K. K., Kumari, A., and Haque, S. (2016). Concomitant production of lipids and carotenoids in Rhodosporidium toruloides under osmotic stress using response surface methodology. Front. Microbiol. 7:1686. doi: 10.3389/fmicb.2016.01686
Singh, G., Sinha, S., Bandyopadhyay, K. K., Kumar, R., and Paul, D. (2018). Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste ‘extract' for enhanced and concomitant lipid and β-carotene production. Microb. Cell Fact. 17:182. doi: 10.1186/s12934-018-1026-4
Singh, G., Sinha, S., Kumar, K. K., Gaur, N. A., Bandyopadhyay, K. K., and Paul, D. (2020). High density cultivation of oleaginous yeast isolates in 'mandi' waste for enhanced lipid production using sugarcane molasses as feed. Fuel 276:118073. doi: 10.1016/j.fuel.2020.118073
Singh, N., Dhanya, B. S., and Verma, M. L. (2020). Nano-immobilized biocatalysts and their potential biotechnological applications in bioenergy production. Mater. Sci. Energy Technol. 3, 808–824. doi: 10.1016/j.mset.2020.09.006
Sinha, S., Singh, G., Arora, A., and Paul, D. (2020). Carotenoid production by red yeast isolates grown in agricultural and “mandi” waste". Waste Biomass Valor. 11, 3939–3949. doi: 10.1007/s12649-020-01288-8
Sinha, S., Singh, S., and Paul, D. (2021). Lipid and carotenoid production by Rhodosporidium toruloides ATCC204091 using C5 and C6 sugars obtained from lignocellulosic hydrolysate. J. Env. Biol. 42, 938–944. doi: 10.22438/jeb/42/4/MRN-1583
Keywords: biofuel, advanced, bioprocess, cost effective, environment friendly
Citation: Paul D, Arora A and Verma ML (2021) Editorial: Advances in Microbial Biofuel Production. Front. Microbiol. 12:746216. doi: 10.3389/fmicb.2021.746216
Received: 23 July 2021; Accepted: 30 August 2021;
Published: 28 September 2021.
Edited by:
Eric Altermann, AgResearch Ltd., New ZealandReviewed by:
Ajar Nath Yadav, Eternal University, IndiaDivjot Kour, Southern Federal University, Russia
Copyright © 2021 Paul, Arora and Verma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Debarati Paul, drdebaratipaul@gmail.com; Anju Arora, anjudev@yahoo.com; Madan L. Verma, madanverma@gmail.com; madanverma@iiitu.ac.in