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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1413120

Identification and Characterization of a Potential Strain for the Production of Polyhydroxyalkanoate From Glycerol

Provisionally accepted
Mengheng Xue Mengheng Xue 1Rong Huang Rong Huang 2Wei Liu Wei Liu 1Jian Cheng Jian Cheng 3Yuwan Liu Yuwan Liu 3Jie Zhang Jie Zhang 3Dingyu Liu Dingyu Liu 3*Huifeng Jiang Huifeng Jiang 3*
  • 1 School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hebei Province, China
  • 2 College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
  • 3 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China

The final, formatted version of the article will be published soon.

    While poly(3-hydroxybutyrate) (PHB) holds promise as a bioplastic, its commercial utilization has been hampered by the high cost of raw materials. However, glycerol emerges as a viable feedstock for PHB production, offering a sustainable production approach and substantial cost reduction potential. Glycerol stands out as a promising feedstock for PHB production, offering a pathway towards sustainable manufacturing and considerable cost savings. The identification and characterization of strains capable of converting glycerol into PHB represent a pivotal strategy in advancing PHB production research. In this study, we isolated a strain, Ralstonia sp. RRA (RRA). The strain exhibits remarkable proficiency in synthesizing PHB from glycerol. With glycerol as the carbon source, RRA achieved a specific growth rate of 0.19 h -1 , attaining a PHB content of approximately 50% within 30 hours. Through thirdgeneration genome and transcriptome sequencing, we elucidated the genome composition and identified a total of 8 genes (glpR, glpD, glpS, glpT, glpP, glpQ, glpV, glpK) involved in the glycerol metabolism pathway. Leveraging these findings, the strain RRA demonstrates significant promise in producing PHB from low-cost renewable carbon sources.

    Keywords: PHB, Polyhydroxyalkanoates, Ralstonia sp., glycerol utilisation, Third-generation sequencing

    Received: 06 Apr 2024; Accepted: 20 May 2024.

    Copyright: © 2024 Xue, Huang, Liu, Cheng, Liu, Zhang, Liu and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Dingyu Liu, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China
    Huifeng Jiang, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China

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