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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Neurol. | doi: 10.3389/fneur.2019.00945

MMP-9-related microRNAs as prognostic markers for hemorrhagic transformation in cardioembolic stroke patients

 Lukai Zheng1, Yao Xiong1, Junfeng Liu1, Xue Yang1,  Lu Wang1,  Shuting Zhang2, Ming Liu1 and  Deren Wang1*
  • 1Center of Cerebrovascular Diseases, West China Hospital, Sichuan University, China
  • 2Department of Neurology, Center of Cerebrovascular Diseases, West China Hospital, Sichuan University, China

Studies suggest that microRNAs that regulate expression of matrix metalloproteinase (MMP)-9 may be involved in hemorrhagic transformation (HT) after cardioembolic stroke, so we examined whether such microRNAs could predict HT in acute cardioembolic stroke patients. Blood samples were prospectively collected from patients who later experienced HT (n =29) or did not (n =29), and the samples were assayed for eight microRNAs identified as related to MMP-9 based on three microRNA databases. Expression levels of these microRNAs were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) in 28 of the 58 patients, 14 of whom suffered HT and 14 of whom did not. Four differentially expressed miRNAs were identified: hsa-miR-21-5p, hsa-miR-206, hsa-miR-491-5p and hsa-miR-3123. Subsequent qRT-PCR analysis of these four miRNAs across all 58 patients showed that levels of miR-21-5p, miR-206, and miR-3123 were significantly higher in patients with HT than in those without HT, while expression of miR-491-5p was similar between the two groups. The area under the receiver operating characteristic curve for predicting HT was 0.677 (95% CI 0.535-0.818) for miR-21-5p, 0.687 (95% CI 0.543-0.830) for miR-206, and 0.661 (95% CI 0.512-0.810) for miR-3123. Our results suggest that these three microRNAs may be prognostic markers for HT after cardioembolic stroke, which should be verified by future studies with large samples.

Keywords: microRNA (miR), Hemorrhagic transformation (HT), Cardioembolic stroke, MMP-9 = matrix metalloprotease-9, prediction

Received: 13 Mar 2019; Accepted: 15 Aug 2019.

Edited by:

Jean-Claude Baron, University of Cambridge, United Kingdom

Reviewed by:

Nikolaus Plesnila, Institute for Stroke and Dementia Research (ISD), Germany
Xiaoying Wang, School of Medicine, Tulane University, United States  

Copyright: © 2019 Zheng, Xiong, Liu, Yang, Wang, Zhang, Liu and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: MD, PhD. Deren Wang, Center of Cerebrovascular Diseases, West China Hospital, Sichuan University, Chengdu, China,