@ARTICLE{10.3389/fnins.2016.00315, AUTHOR={Colombi, Ilaria and Tinarelli, Federico and Pasquale, Valentina and Tucci, Valter and Chiappalone, Michela}, TITLE={A Simplified In vitro Experimental Model Encompasses the Essential Features of Sleep}, JOURNAL={Frontiers in Neuroscience}, VOLUME={10}, YEAR={2016}, URL={https://www.frontiersin.org/articles/10.3389/fnins.2016.00315}, DOI={10.3389/fnins.2016.00315}, ISSN={1662-453X}, ABSTRACT={In this paper, we show that neuronal assemblies plated on Micro Electrode Arrays present synchronized, low frequency firing patterns similar to in vivo slow wave oscillations, which are a key parameter of sleep-like state. Although neuronal cultures lack the characteristic high-frequency waves of wakefulness, it is possible to modulate their spontaneous firing pattern through the administration of specific neurotransmitters such as acetylcholine. We thus stimulated the cortical cultures with an agonist of acetylcholine receptor, Carbachol, which caused a desynchronization of the spontaneous firing of the cultures. We recorded and monitored the cultures for a period of over 31 h. We analyzed the electrophysiological signals by exploiting novel methodological approaches, taking into account the different temporal scales of the recorded signals, and considering both spikes and local field potentials. Supporting the electrophysiological analysis results, gene expressions of targeted genes showed the activation of specific markers involved in sleep-wake rhythms. Our results demonstrate that the Carbachol treatment induces desynchronization of neuronal activity, altering sleep-like properties in an in vitro model.} }