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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Neurosci. | doi: 10.3389/fnins.2019.01032

Enriched environmental conditions modify the gut microbiome composition and fecal markers of inflammation in Parkinson’s disease

 Yogesh Singh1, 2, Mohammed E. Hadidi3, Jakob Matthes1, Zinah Wassouf1, Julia Schulz-Henrich1, Ursula Kohlhofer1,  Leticia Quintanilla-Martinez1, Daniel Huson4,  Olaf Riess2* and  Nicolas Casadei2*
  • 1Tübingen University Hospital, Germany
  • 2Institute of Medical Genetics and Applied Genomics, Tübingen University Hospital, Germany
  • 3Nile University, Egypt
  • 4Applied Bioinformatics Group, Faculty of Mathematics and Natural Sciences, University of Tübingen, Germany

Recent findings suggest an implication of the gut microbiome in Parkinson’s disease (PD) patients. PD onset and progression has also been linked with various environmental factors such as physical activity, exposure to pesticides, head injury, nicotine, and dietary factors. In this study, we used a mouse model overexpressing the complete human SNCA genes (SNCA-TG mice) modeling familial and sporadic forms of PD to study whether environmental conditions such as standard versus enriched environment changes the gut microbiome and influences disease progression. We performed 16S rRNA DNA sequencing on fecal samples for microbiome analysis and studied fecal inflammatory calprotectin from the colon of control and SNCA-TG mice kept under standard environment (SE) and enriched environment (EE) conditions. The overall composition of the gut microbiota was not changed in SNCA-TG mice compared with WT in EE with respect to SE, however, individual gut bacteria at genus level such as Lactobacillus sp. were significantly changed in SNCA-TG mice. EE significantly reduced colon fecal inflammatory calprotectin protein in WT and SNCA-TG EE compared to SE. Moreover, EE reduces the pro-inflammatory cytokines in the feces and inflammation inducing genes in the colon. Our data suggest that an enriched social environment has a positive effect on the induction of SNCA mediated inflammation in the intestine and by modulating anti-inflammatory gut bacteria.

Keywords: PD, microbiome, a-syn, calprotectin, 16S rRNA, enriched environment

Received: 19 May 2019; Accepted: 12 Sep 2019.

Copyright: © 2019 Singh, Hadidi, Matthes, Wassouf, Schulz-Henrich, Kohlhofer, Quintanilla-Martinez, Huson, Riess and Casadei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Olaf Riess, Institute of Medical Genetics and Applied Genomics, Tübingen University Hospital, Tübingen, Baden-Württemberg, Germany, olaf.riess@med.uni-tuebingen.de
Mx. Nicolas Casadei, Institute of Medical Genetics and Applied Genomics, Tübingen University Hospital, Tübingen, Baden-Württemberg, Germany, nicolas.casadei@med.uni-tuebingen.de