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Front. Neurosci.
Sec. Neurogenomics
Volume 17 - 2023 | doi: 10.3389/fnins.2023.1174951

Circulating plasma miR-23b-3p as a biomarker target for Idiopathic Parkinson’s disease: comparison with small extracellular vesicle miRNA

 Sanskriti Rai1 Prahalad S. Bharti1 Rishabh Singh1 Simran Rastogi1 Komal Rani2 Gyan P. Modi3  Krishna K. Inampudi1  Hem C. Pandey4 Sanjay Yadav5  Roopa Rajan6  Fredrik Nikolajeff7  Saroj Kumar1, 8*
  • 1Department of Biophysics, All India Institute of Medical Sciences, India
  • 2Department of Pathology & Laboratory Medicine, All india institute of medical sciences, Bibinagar, India
  • 3Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), India
  • 4Department of Transfusion Medicine, All India Institute of Medical Sciences, India
  • 5Department of Biochemistry, All India Institute of Medical Sciences Raebareli, India
  • 6Department of Neurology, All India Institute of Medical Sciences, India
  • 7Department of Health, Education and Technology, Luleå University of Technology, Sweden
  • 8Luleå University of Technology, Sweden

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Background: Parkinson’s disease (PD) is an increasingly common neurodegenerative condition, which causes movement dysfunction and a broad range of nonmotor symptoms. There is no molecular or biochemical diagnosis test for PD. The miRNAs are a class of small non-coding RNAs and are extensively studied owing to their altered expression in pathological states and facile harvesting and analysis techniques.
Methods: A total of 48 samples (16 each of PD, aged-matched and young controls) were recruited. The small extracellular vesicles (sEV) were isolated and validated using western blot, transmission electron microscope, and nanoparticle tracking analysis. Small RNA isolation, library preparation, and small RNA sequencing followed by differential expression and targeted prediction of miRNA were performed. The real-time PCR was performed with the targeted miRNA on PD, aged-matched, and young healthy control of plasma and plasma-derived sEV to demonstrate their potential as a diagnostic biomarker.
Results: In RNA sequencing, we identified 14.89% up-regulated (fold change 1.11 to 11.04, p<0.05) and 16.54% downregulated (fold change -1.04 to -7.28, p<0.05) miRNAs in PD and controls. Four differentially expressed miRNAs (miR-23b-3p, miR-29a-3p, miR-19b-3p, and miR-150-3p) were selected. The expression of miR-23b-3p was “upregulated” (p=0.002) in plasma whereas “downregulated” (p=0.0284) in plasma-derived sEVs in PD than age-matched controls. The ROC analysis of miR-23b-3p revealed better AUC values in plasma (AUC= 0.8086, p=0.0029) and plasma-derived sEVs (AUC= 0.7278, p= 0.0483) of PD and age-matched controls.
Conclusion: We observed an opposite expression profile of miR-23b-3p in PD and aged-matched healthy control in plasma and plasma-derived sEV fractions, where the expression of miR-23b-3p is increased in PD plasma while decreased in plasma-derived sEVs fraction. We further observed the different miR-23b-3p expression profiles in the young and aged-matched healthy control.

Keywords: Parkinsion’s disease (PD), Small extracellular vesicle (sEV), miRNA - microRNA, miR-23b-3p, biomarker

Received: 27 Feb 2023; Accepted: 08 Aug 2023.

Copyright: © 2023 Rai, Bharti, Singh, Rastogi, Rani, Modi, Inampudi, Pandey, Yadav, Rajan, Nikolajeff and Kumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Saroj Kumar, All India Institute of Medical Sciences, Department of Biophysics, New Delhi, 110 029, National Capital Territory of Delhi, India