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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pediatr. | doi: 10.3389/fped.2019.00288

MiR-223-3p alleviates vascular endothelial injury by targeting IL6ST in Kawasaki disease

 Xiang Wang1, 2, yue y. Ding1, ye Chen1, Qin q. Xu1,  Hui g. Qian1, 3, Guo w. Qian1, Lei Cao1, Ping w. Zhou1,  Miao Hou4 and Tao h. Lv1*
  • 1Children’s hospital of Soochow university, Suzhou University, China
  • 2Department of Pediatrics, Huai'an First People's Hospital, China
  • 3Soochow University, China
  • 4department of cardiology , Children’s hospital of Soochow university, China

Kawasaki disease (KD) is an acute, self-limiting systemic vascular inflammatory disorder.It causes pathological changes majorly in small and medium-sized arteries, especially the coronary arteries, which increases the risk of developing coronary heart disease as adults. miR-223-3p plays important roles in many diseases. However, less research was focused on the relationship between miR-223-3p and KD. In this study, we found that the expression of miR-223 was significantly high in serum from patients with Kawasaki disease (KD) and KD serum could increase miR-223 transcription levels in human coronary artery endothelial cells(HCAECs). MiR-223 could alleviate vascular endothelial damage in KD mice, and IL-6, E-selectin and ICAM-1 were negative in the sametime. Luciferase report assay showed that IL6ST is a target gene of miR-223. Overexpression of miR-223 downregulated IL6ST expression, and decreased the expression of p-STAT3 and NF-κB, while miR-223 inhibitor could reverse the above process. Taken together, the results suggest that miR-223 has an essential protective role in KD vascular endothelial injury by targeting IL6ST, and the miR-223 may be used as a potential biomarker for diagnosis of KD.

Keywords: kawasaki disease, MicroRNA-223, IL6ST, Vascular endothelial damage, stat3

Received: 19 Feb 2019; Accepted: 26 Jun 2019.

Edited by:

Hongfang Jin, Peking University First Hospital, China

Reviewed by:

Giuseppina Milano, Lausanne University Hospital (CHUV), Switzerland
Zhong-Dong Du, Beijing Children’s Hospital, Capital Medical University, China
Wei Sheng, Children's Hospital, Fudan University, China  

Copyright: © 2019 Wang, Ding, Chen, Xu, Qian, Qian, Cao, Zhou, Hou and Lv. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Tao h. Lv, Suzhou University, Children’s hospital of Soochow university, Suzhou, China, haitaosz@163.com