Original Research ARTICLE
Eco-friendly extraction of Sinapine from residues of mustard production
- 1AgroParisTech Institut des Sciences et Industries du Vivant et de L'environnement, France
In this study, our efforts were focused on the optimization of phenolic compounds - mainly sinapine - extraction from residues of industrial mustard production. A preliminary work was conducted to discuss the way data from Folin-Ciocalteu analytical method are reported and exploited in the literature, and to determine whether methanol can be replaced by ethanol as extracting solvent. As this first question had found a positive answer, an optimization of the extraction process on crude and pretreated industrial residue was achieved using a Response Surface Methodology (RSM). Optimal conditions obtained through this Design of Experiments (DoE) allowed the extraction of more than 10 mg/g of defatted and dried matter (aka DDM) of phenolics from defatted and dried matter, and 13 mg/g DDM of phenolics from non-pretreated matter (results expressed as equivalent of DDM).
Keywords: mustard, Sinapine, Phenols, response surface (RS) methodology, optimization, Folin-ciocalteau assay
Received: 30 Jul 2018;
Accepted: 12 Feb 2019.
Edited by:Maria Pilar Bernal, Center for Edaphology and Applied Biology of Segura, Spanish National Research Council (CSIC), Spain
Reviewed by:Helen Treichel, Universidade Federal da Fronteira Sul, Brazil
Sabine Houot, Institut National de la Recherche Agronomique (INRA), France
Copyright: © 2019 Flourat, Willig, Teixeira and Allais. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Ms. Amandine L. Flourat, AgroParisTech Institut des Sciences et Industries du Vivant et de L'environnement, Paris, 75231, France, firstname.lastname@example.org
Prof. Florent Allais, AgroParisTech Institut des Sciences et Industries du Vivant et de L'environnement, Paris, 75231, France, email@example.com