Physiologic Factors Influencing the Arterial-To-End-Tidal CO2 Difference and the Alveolar Dead Space Fraction in Spontaneously Breathing Anesthetised Horses

Introduction

Ventilation/perfusion (V/Q) mismatch develops rapidly in anesthetized horses (1). Venous admixture and pulmonary dead space mark the two “extremes” of the spectrum of this mismatch; the former representing perfused but not ventilated alveoli (V/Q = 0) while the latter representing alveoli which are only ventilated but not perfused (V/Q = ∞).

Several oxygen indices including arterial-to-alveolar partial pressure of oxygen (PO₂) difference and P_aO₂/inspiratory fraction of oxygen (FiO₂) ratio or f-shunt have been tested to estimate venous admixture in people and horses (2) and are frequently applied in clinical routine. The index used to estimate physiologic dead space is the difference between the arterial and end-tidal partial pressure of CO₂ [P_(a-ET)CO₂], which was evaluated in spontaneously breathing and ventilated people several decades ago (3). The P_(a-ET)CO₂ was divided by P_aCO₂ and denoted as an estimate of alveolar dead space, and called alveolar dead space fraction [VDalv_frac = P_(a-ET)CO₂/P_aCO₂] (4). The concept of using P_aCO₂ and P_ETCO₂ to estimate “dead space” was adapted in equine anesthesia over 40 years ago (5, 6) and has been used extensively ever since to estimate “dead space” in many animal species (7).

“True” dead space measurements, however, include the measurement of mixed-expired partial pressure of CO₂ (P_ĒCO₂) and the mean alveolar CO₂ (P_ACO₂) (8). Bohr used these two CO₂ measurements to calculate the famous physiologic dead space ratio [VD_Bohr = (P_ACO₂ − P_ĒCO₂)/P_ACO₂]. As P_ACO₂ was difficult to obtain, Enghoff suggested more than 50 years later that the partial pressure of CO₂ in the arterial blood would be representative of the P_ACO₂. Therefore, he modified Bohr’s formula substituting P_ACO₂ by P_aCO₂ to derive the following formula: VD_Eng = (P_aCO₂ − P_ĒCO₂)/P_aCO₂ (9). What Enghoff failed to account for is the fraction of shunting blood volume that cannot unload CO₂ during lung passage and thereby adding CO₂-rich blood to the arterial side (10). This fact is the reason why P_aCO₂ almost invariably exceeds P_ACO₂. The contribution of this blood flow equals venous admixture and has been shown to cause VD_Eng to overestimate the “true” physiologic dead space (11–13). On a positive note, it should be used as a global index of V/Q mismatching since VD_Eng includes pulmonary dead space and venous admixture alike (10, 12). It is important to note that all studies concluding that P_(a-ET)CO₂ and VDalv_frac is valid representative of pulmonary dead space used VD_Eng as their reference values for dead space (3–5). As VD_Eng does not represent the “true” physiologic dead space, it is not surprising that more recent studies show the unreliability of P_(a-ET)CO₂ and VDalv_frac to predict dead space or changes thereof (14–16). Following aforementioned assumptions the main factors causing an inaccuracy in dead space calculation using P_(a-ET)CO₂ and VDalv_frac are venous admixture and atelectasis formation (13, 17, 18). Furthermore, other physiologic factors like tidal volume, oxygen consumption, respiratory rate, and homogeneity of lung emptying have been shown to change the difference between the arterial and the end-tidal CO₂ values (14, 15, 19). To account for the fact that Enghoff’s “dead space” is influenced by V/Q = ∞, V/Q = 0 and the global V/Q mismatch, for clarity, the term will be referred to as Enghoff’s approach (V/Q_Eng) in this manuscript.

Based on the above considerations, there is a clinical need for a reliable and yet easy to obtain alternative index of dead space overcoming the time consuming difficult measurement of CO₂ variables needed for Bohr’s equation. Until two decades ago, mixed-expired CO₂, which is employed in Bohr’s equation and Enghoff’s approach, could only be measured by using a mixing chamber or Douglas bag (20). More recently, volumetric capnography (VCap) provides an easier and clinically more convenient way to measure P_ĒCO2. To obtain the VCap curve the partial pressure of CO₂ is plotted over the expired tidal volume—therefore the term VCap (13). P_ĒCO₂ is the average of the partial pressure of CO₂ during exhalation. It is calculated from the area under the curve (the integral of the VCap curve during exhalation) divided by tidal volume (10). Beside P_ĒCO₂ also P_ACO₂ and airway dead space can be derived from the VCap curve (Figure 1) (21). For this evaluation, the exhaled gas flow and partial pressure of CO₂ needs to be measured simultaneously. Due to the time delay of the CO₂ sensor in side-stream capnography, a mainstream CO₂ sensor combined with a pneumotachograph close to the endotracheal tube (ETT) is the most commonly used set-up to measure VCap. The VCap monitors developed for humans cannot cope with the high inspiratory and expiratory flow rates in horses. In order to allow the use of these pneumotachographs during equine anesthesia a custom-made flow-partitioning device can be inserted between ETT and the Y-piece of the anesthetic breathing circle (22). This device splits the flow into four equal parts, making it possible to calculate the correct volume by multiplying the measured values by four.

FIGURE 1

Figure 1. Graphical illustration of a volumetric capnography (VCap) curve where the partial pressure of expired CO₂ (Exp. CO₂) is plotted over the volume of one tidal breath (VT). “A” is the inflection point of VCap that separates per definition the airways from alveolar compartment and is used to calculate airway dead space (VDaw). PACO₂ is the CO₂ value at the midpoint between the inflection point “A” and the end-tidal CO₂ (P_ETCO₂). The mixed-expired CO₂ (P_E¯CO₂) represents the mean CO₂ in the expired gas. The difference between P_ETCO₂ and arterial oxygen partial pressure (PaCO₂) is plotted as well.

The aim of this study was to discern factors that best explain the variability in P_(a-ET)CO₂ and VDalv_frac in anesthetized spontaneously breathing horses in dorsal recumbency. We hypothesized that: (1) P_(a-ET)CO₂ and VDalv_frac is strongly associated with V/Q_Eng and derived variables, but much less with “true” dead space variables derived from Bohr’s original formula, (2) P_(a-ET)CO₂ and VDalv_frac is highly influenced by venous admixture and other factors influencing lung perfusion.

Materials and Methods

This experimental study had ethical approval by the local committee for animal experimentation of the Swiss government (TV-4985). It was part of an original study performed to evaluate the effects of CPAP in anesthetized horses (23). Only those horses in which no CPAP was applied were included in this current study.

Animals

Six horses were included in this experimental study out of the control group of a former study (23). Horses were acquired from local breeders and housed in boxes at the hospital for at least a week before commencing this study. All horses were considered healthy based on thorough clinical examination, routine blood work, and arterial blood gas analysis before anesthesia. They were fasted for 12 h, but had free access to water until 2 h prior to induction.

Before premedication, a 8F 110-cm angiography balloon catheter (Arrow Swiss, Edwards Lifesciences, Switzerland) was placed via the jugular vein in the pulmonary artery using pressure guidance to determine correct positioning.

Anesthesia

Premedication consisted of medetomidine (0.007 mg kg⁻¹) (Dorbene, Graeub AG, Switzerland) IV and phenylbutazone (4 mg kg⁻¹) IV (Butadion, Streuli Pharma AG, Switzerland). Anesthesia was induced with diazepam (0.02 mg kg⁻¹) (Valium, Roche, Switzerland) and ketamine (2 mg kg⁻¹) (Ketanarkon 100, Streuli Pharma AG, Switzerland) IV. After induction, a 26-mm internal diameter cuffed silicone ETT was inserted into the trachea and connected to a circle breathing system (Tafonius, Vetronic Services Ltd., UK) after placing the horse on a mattress in dorsal recumbency. Anesthesia was maintained using isoflurane (Attane Isoflurane, Provet, Switzerland) in an oxygen/air mixture (inspiratory O₂ fraction: FiO₂ = 0.5). Medetomidine was infused as a continuous rate infusion (0.0035 mg kg⁻¹ h⁻¹). Ketamine (50–100 mg bolus) was administered IV in case of spontaneous movement.

Two multi-parameter monitors (Datex-Ohmeda S/3 Anesthesia Monitor, Datex-Ohmeda and Solomon monitoring system, Vetronics, UK) were used to measure standard cardiopulmonary parameters throughout anesthesia as well as mean pulmonary artery pressure. The pressure transducer was zeroed to atmospheric pressure and leveled at the height of the shoulder joint. New pressure transducers were used for each horse.

Ringer’s lactate (Ringer-Lactat Fresenius, Fresenius Kabi, Switzerland) was infused at a rate of 10 mL kg⁻¹ h⁻¹ and hydroxyethyl starch (HAES-steril 10% ad us. vet., Fresenius Kabi, Switzerland) was started 2 h after induction (1 mL kg⁻¹ h⁻¹) to account for the expected fluid losses due to medetomidine. Dobutamine was infused intravenously to maintain mean arterial pressure between 75 and 85 mmHg.

If arterial PCO₂ (P_aCO₂) exceeded 100 mmHg (13.3 kPa) at any measurement point horses were excluded from further data acquisition and mechanical ventilation was started.

In the original study all horses were anesthetized for 6 h but only data from the first 3 h were used in this study. At the end of the study period, horses were allowed to recover unassisted.

Monitoring and Data Collection

All cardiovascular data including mean pulmonary pressure (MPP) were recorded every 30 min during the first 3 h of anesthesia (T30, T60, T90, T120, T150, T180). Cardiac output (CO) was measured in triplicate at T60, T120, and T180 using the lithium dilution technique (LiDCO; LiDCO Group, UK) (24).

A flow-partitioning device was placed between the ETT and the Y-piece of the circle system dividing the total flow into four parts by two flow splitting adapters connected to each other by means of four silicon tubes equipped with four identical human adult NICO capnograph connectors (Respironics, Wallingford, CT, USA) (22). These connectors include a fixed-orifice resistance for flow measurement and a connection site for the mainstream sensor of the capnography unit of the NICO. Only one of the four connectors was connected to the spirometer and mainstream capnography unit of the NICO.

At time points of cardiovascular evaluation, VCap and spirometry data were recorded for 3 min using the dedicated software Datacoll (Respironics, Wallingford, CT, USA). Furthermore, simultaneous arterial and mixed venous blood samples were taken under anaerobic conditions at each of these time points and PO₂, carbon dioxide tension (PCO₂) and arterial hemoglobin concentration ([Hb]) analyzed immediately (Rapidpoint, Siemens, Switzerland).

All devices were calibrated following manufacturer’s guidelines before each anesthesia; the capnograph of the NICO device was calibrated with room air before each experiment (infrared sensor with a response time <60 ms and accuracy of ±2 mmHg) and the accuracy of the pneumotachograph was verified with a 100-mL calibration syringe before and after all measurements (allowed accuracy of ±3% following manufacturers guidelines for the calibration set-up). The accuracy of the volume measurement of the flow-partioning device used in our set-up in connection with the NICO device was verified in an in vitro experiment published elsewhere (25). The difference between the reading of the flow-partitioning device in combination with the NICO device and the 10 L calibration syringe was 0.73 ± 4.3% (mean ± SD).

Data Analysis

After importing CO₂ values into an Excel spread sheet, P_(a-ET)CO₂ and VDalv_frac were calculated. VCap data were imported into a custom-made macro routine in Excel (Excel; Microsoft Corporation, WA, USA) and the recorded breaths analyzed. The mean value of all recorded breaths per time point and horse was used for statistical analysis. Bohr’s dead space ratio (VD_Bohr) and V/Q_Eng were calculated following curve approximation by the solver function according to Tusman et al. (26). The ratio of alveolar dead space (difference between physiologic VDs and VD_aw) to alveolar tidal volume was calculated based on Bohr’s (VD_alvBohr/VT_alv) and Enghoff’s (V/Q_alvEng/VT_alv) respective equations. Airway dead space was defined as the volume at the inflection point of the VCap curve and was normalized by the tidal volume (VD_aw/VT). The slope of phase III of the volumetric capnogram (SIII) was determined. Venous admixture $(\dot{Q}s\text{}/\text{}\dot{Q}t)$ and oxygen consumption (VO₂) were calculated using standard equations (27).

Statistics

Continuous data were summarized and reported as mean ± SD and range. All continuous variables were deemed to follow a normal distribution based on Q–Q plots and failure to reject the null hypothesis of normality was tested using Shapiro–Wilk’s statistic at P ≤ 0.05.

For the purpose of explaining the variance of the responses P_(a-ET)CO₂ and VDalv_frac, the explanatory variables were grouped according to what physiologic state they best represented; VD_Bohr, VD_alvBohr/VT_alv, and VD_aw/VT were considered “true” dead space variables; CO and MPP were considered variables related to pulmonary blood flow; $\dot{Q}s\text{}/\text{}\dot{Q}t$ was considered a functional variable mirroring collapsed lung areas with low V/Q causing CO₂ admixture to the arterial side; SIII was treated as a factor characterizing homogenity of lung emptying; and VT and VO₂ were considered possible explanatory variables based on evidence in the literature.

Initial explorations of the association between Enghoff’s variables (V/Q_Eng, V/Q_alvEng/VT_alv) and P_(a-ET)CO₂ and VDalv_frac were performed using Pearson’s correlation with rho reported for each variable. All data points from each horse were used. Multiple linear regression analysis was then performed on P_(a-ET)CO₂ and VDalv_frac, assessing all combinations of explanatory variables to determine which explanatory variables best explained the variance of each response. The simplest, best-fit model was selected based on the maximum adjusted R² and the smallest Mallow’s p (C_p). The R² of the selected model, representing how much of the variance in the response could be explained by the variance of the selected variables, was reported. Enghoff’s approach and the associated variables (representing high and low V/Q ratios) were not included in the multi-variate analysis as the aim of this analysis was to show if the two indices represent V/Q = ∞ and areas of high V/Q ratio, or V/Q = 0 and areas of low V/Q ratio.

Results

Six healthy adult horses with a mean (SD) age of 10 ± 6 years and body weight of 539 ± 35 kg (one Thoroughbred, five Freiberger) completed the 3 h study period and complete data sets of all horses could be analyzed.

Respiratory variables, VCap, and specific cardiovascular data of all horses are given in Table 1.

TABLE 1

Table 1. Mean ± SD of the arterial to end-tidal CO₂ difference, alveolar dead space fraction, dead space and cardiorespiratory parameters in six spontaneously breathing horses under general isoflurane anesthesia in dorsal recumbency at different time points (T30–180).

The P_(a-ET) CO₂ ranged from −1 to 39 mmHg with the highest values at T120 (one horse), T150 (two horses), and T180 (three horses).

There was a high correlation of V/Q_alvEng/VT_alv and a moderate correlation of V/Q_Eng, $\dot{Q}s\text{}/\text{}\dot{Q}t$, and CO with P_(a-ET) CO₂ (Figure 2; Table 2).

FIGURE 2

Figure 2. Association between arterial to end-tidal CO₂ difference (P_(a-ET)CO₂) and venous admixture $(\dot{Q}s\text{}/\text{}\dot{Q}t)$ in six spontaneously breathing horses under general isoflurane anesthesia in dorsal recumbency. The graph demonstrates the relation over a wide range of $\dot{Q}s\text{}/\text{}\dot{Q}t$ values.

TABLE 2

Table 2. Pearson’s correlation coefficient (rho) for the arterial to end-tidal CO₂ difference and alveolar dead space fraction with dead space and cardiorespiratory parameters in six spontaneously breathing horses under general isoflurane anesthesia in dorsal recumbency.

The variance of P_(a-ET)CO₂ was best explained by $\dot{Q}s\text{}/\text{}\dot{Q}t$ and CO, accounting for 75% (R² = 0.7516). Adding MPP to the model improved the explanation by additional 2% (R² = 0.7860) (Table 3).

TABLE 3

Table 3. The results of multi linear regression analysis for factors associated with the arterial to end-tidal CO₂ difference (P_(a-ET)CO₂) and alveolar dead space fraction (VDalv_frac) in six spontaneously breathing horses under general isoflurane anesthesia in dorsal recumbency.

VDalv_frac ranged from −0.02 to 0.36 with the highest values observed at the same time points as the highest P_(a-ET)CO₂, except in one horse. This horse had the highest P_(a-ET)CO₂ earlier than the others showing consistently lower COs than all other horses during the entire study period.

Correlation of VDalv_frac with V/Q_alvEng/VT_alv was high and moderate with V/Q_Eng and $\dot{Q}s\text{}/\text{}\dot{Q}t$ (Figure 3; Table 2).

FIGURE 3

Figure 3. Association between alveolar dead space fraction (VDalv_frac) and venous admixture $(\dot{Q}s\text{}/\text{}\dot{Q}t)$ in six spontaneously breathing horses under general isoflurane anesthesia in dorsal recumbency. The graph demonstrates the relation over a wide range of $\dot{Q}s\text{}/\text{}\dot{Q}t$ values.

The variance of VDalv_frac was best explained by $\dot{Q}s\text{}/\text{}\dot{Q}t$ and CO accounting for 71% of it (R² = 0.7115). Adding VD_aw/VT to the model improved the explanation by another 3% (R² = 0.7444) (Table 3).

Neither Bohr’s dead space variables, nor SIII, VT, or VO₂ showed any significant correlation or provided any further explanation of the variance of P_(a-ET)CO₂ and VDalv_frac.

Discussion

The results of this study support the first hypothesis in that both indices, P_(a-ET)CO₂ and VDalv_frac can be used to estimate Enghoff’s approach, but do not represent “true” dead spaces as derived from Bohr’s equation in spontaneously breathing horses. The results of this study also support the second hypothesis in that venous admixture and factors related to pulmonary perfusion, like CO and pulmonary pressure influence P_(a-ET)CO₂ and VDalv_frac. Therefore, P_(a-ET)CO₂ and VDalv_frac did not represent V/Q = ∞ (physiologic or alveolar dead space) in these spontaneously breathing horses in dorsal recumbency, but a global mismatch in the pulmonary ventilation/perfusion ratio, a finding supported by studies in other species.

Looking at the difference between PaCO₂ and ETCO₂ from the theoretical side every increase of the difference can have two causes: (1) a decrease in ETCO₂ relative to PaCO₂ or (2) any increase in PaCO₂ relative to ETCO₂. In the following, we want to discuss the physiologic background for both causes related to the results found in this study.

(1) A drop in ETCO₂ in relation to PaCO₂ is indicative of an increase in ventilation of non-perfused (V/Q = ∞; alveolar dead space) or poorly perfused (areas of high V/Q ratio) alveoli, resulting in an increase in physiologic dead space. This fact directed Nunn and Hill (3) and Severinghaus et al. (4) toward the conclusion that physiologic or alveolar dead space could be estimated from these two parameters.

No drop in ETCO₂ was seen in any of our horses at any time point. In fact ETCO₂ increased continuously together with PaCO₂ between the measurement points in all horses (Table 1). This indicates that physiologic dead space itself did not increase at all, or if so, only marginally. The VCap results confirmed this by showing only small changes in Bohr’s physiologic and alveolar dead space values throughout the study period. The relatively small amount of “real” physiologic and alveolar dead space and minimal changes could be expected in healthy horses with high CO under spontaneous ventilation. We consider this the most likely reason why we did not find any interaction between the two CO₂ indices and our measured dead space variables. However, in states of low CO, overdistension of alveolar tissue during positive pressure ventilation, excessive PEEP or pulmonary thromboembolism the amount of well-ventilated but poorly or non-perfused alveoli (high V/Q and high Bohr’s alveolar dead space) will increase (10, 19, 27–29). These changes also affect V/Q_Eng as a factor for the global V/Q mismatch including dead space. This has been shown in a horse suffering from venous air embolism where V/Q_Eng nearly doubled during the unintended air infusion (29). As the two CO₂ indices highly correlated with V/Q_Eng, we can assume that in situations of high V/Q and high Bohr’s alveolar dead space P_(a-ET)CO₂ and VDalv_frac will also increase. Future studies will need to verify this assumption.

The only dead space variable with a relevant influence on VDalv_frac in our study was airway dead space. This parameter, which neither influences Enghoff’s nor Bohr’s equation, is derived from the VCap curve using mathematical modeling of the expired CO₂ over the measured volume (26). Interestingly this was not the first time airway dead space was identified as a factor influencing P_(a-ET)CO₂ and VDalv_frac; in a model simulating human physiology airway dead space was detected as one of two main factors, the second one being venous admixture, causing inaccuracies in the calculation of alveolar dead space when using these CO₂ indices (15). As our horses were breathing spontaneously, only small changes in VD_aw/VT were expected. However, the impact of VD_aw/VT might become more relevant when using positive pressure ventilation, especially in combination with positive end-expiratory pressure, which has been shown by Lumb and others to increase airway dead space (27, 28). Thus, the increase in VD_aw/VT can possibly increase VDalv_frac under controlled mechanical ventilation.

(2) The second change, which can influence the difference between PaCO₂ and ETCO₂, namely an increase in PaCO₂ in relation to ETCO₂, occurs when CO₂ cannot be eliminated from the blood into the alveoli. This is the case when the blood flows through atelectatic lung tissue or areas with low V/Q ratios. This fact makes P_(a-ET)CO₂ and VDalv_frac highly variable in situations of high venous admixture (15, 30, 31). Our results confirmed the direct influence of venous admixture on P_(a-ET)CO₂ and VDalv_frac in horses with a broad range of venous admixture volumes. As horses develop extensive venous admixture during anesthesia (1), this species is well suited to show the direct influence of this pathological lung condition on the two CO₂ indices in question. Our horses had between 12 and 52% of their CO perfusing poorly or non-ventilated lung units (V/Q = 0; venous admixture). This spectrum represents the expected amount of venous admixture in anesthetized horses in dorsal recumbency (32). This large difference in venous admixture was reflected by a wide range of P_(a-ET)CO₂ and VDalv_frac values. Strang et al. suggested that P_(a-ET)CO₂ can be used as an index of oxygenation as it correlated well with the amount of atelectasis in pigs seen on computed tomography being even more predictive of the amount of atelectasis than the most accepted oxygen index PaO₂/FiO₂ ratio (18). The strong relationship between P_(a-ET)CO₂ and an impaired oxygenation has also been shown by means of pulse oximetry in human cardiac patients (33).

To confirm our hypothesis that P_(a-ET)CO₂ and VDalv_frac are influenced by parameters determining lung perfusion even further, we tested the influence of CO and pulmonary arterial pressure, where especially high CO values increased P_(a-ET)CO₂ and VDalv_frac. The influence of CO on the two CO₂ indices was very likely due to its direct effect on venous admixture. It is known that increases in CO increase pulmonary pressure whereby pulmonary vessels perfusing collapsed lung areas are recruited. The blood flowing through these vessels increase venous admixture (34) also explaining why we saw an effect of pulmonary pressure on the VDalv_frac.

Our results also confirm earlier findings in humans that both CO₂ indices have a good correlation with Enghoff’s approach and derived variables (3). The fact that Enghoff’s approach represents pulmonary dead space as well as venous admixture is well accepted (10, 12, 16, 30, 31, 35). The fact, however, that venous admixture also affects P_(a-ET)CO₂ and VDalv_frac is not even well established in the human anesthesia literature (18, 30, 31) and has not made its way into clinical veterinary anesthesia. This is why the two CO₂ indices are still frequently used in equine anesthesia to estimate pulmonary dead space (36). There are only a few papers specifically studying the two factors (6, 37–39). Most of the variables which have been shown to effect P_(a-ET)CO₂ and VDalv_frac in horses like duration of anesthesia, body weight, dorsal recumbency, and ventilation mode are known to influence venous admixture, CO, and/or pulmonary pressure. Our findings suggest that the idea of P_(a-ET)CO₂ or VDalv_frac being solely representative of dead space is erroneous or at least questionable in cases of high venous admixture typically observed in anesthetized horses.

The high correlation found in this study between the two CO₂ indices with V/Q_Eng and V/Q_alvEng/VT_alv suggests that P_(a-ET)CO₂ or/and VDalv_frac might be good estimates of global V/Q mismatching in horses. This assumption is based on the fact that variables based on Enghoff’s approach have been shown to represent the sum of dead space and venous admixture (10, 16). This might provide equine anesthetist with a new clinical tool to evaluate the lung’s status; a high P_(a-ET)CO₂ or VDalv_frac with an adequate oxygenation (PaO₂) might indicate a high physiological dead space, which points toward low CO and/or overdistension of lung tissue during controlled mechanical ventilation and recruitment maneuvers or even lung embolism (29, 40). On the other hand low PaO₂ values in conjunction with a high P_(a-ET)CO₂ or/and VDalv_frac might point toward high $\dot{Q}s/\dot{Q}t$ probably in combination with high CO and/or pulmonary pressures as seen in our horses. However, further clinical validation in horses is required to support our assumptions.

We also evaluated the influence of other factors like VT, VO₂, and SIII on the two CO₂ indices as they might have caused changes in the arterial and end-tidal CO₂ difference (14, 15, 19). None of the three parameters contributed any significant variance to either CO₂ index. However, we did not see high variability in our data set for VO₂ and SIII.

One limitation of our study is that VCap has not been directly validated in horses against the multiple inert gas elimination technique for the measurement of Bohr’s dead space in the same rigorous way as it has been done for pigs (35). However, as the principle of VCap is based on respiratory physiology, it can be applied to all mammals as long as the trachea and lung follow similar architectures. The special flow-partitioning device for large animals and mathematical correction for the splitting of the tidal volume into four equal parts has already been shown to provide valid VCap parameters in horses (22, 29, 40, 41).

Only six horses were included in this study as the number was set by the primary investigation (23). Hence, no power calculation was performed prior to commencing this study. However, we were able to answer our study question with the collected data sets specifically for healthy spontaneously breathing horses in dorsal recumbency. No inferences can be drawn on how pulmonary disease or mechanical ventilation would affect the two CO₂ indices. Most importantly future studies in horses with higher alveolar Bohr’s dead space need to evaluate the influence of this V/Q mismatch “extreme” on the two studied CO₂ indices.

The anesthetic protocol and inspired gas composition might have influenced the measurements and results might vary with different drug protocols and FiO₂ level. Furthermore the hemoglobin analysis was performed with a photometric method not validated for whole horse blood, which might have induced an error in the absolute values of $\dot{Q}s\text{}/\text{}\dot{Q}t$ and VO₂ reported here.

These limitations may have an impact on the predictor validity of the investigated parameters. Future studies should investigate the effect of the listed limiting factors influencing the measurements and their potential clinical influences on the two CO₂ indices.

Conclusion

Both CO₂ indices, P_(a-ET)CO₂, or VDalv_frac represent global indices of the inefficiency of gas exchange and V/Q mismatching, and both are affected by venous admixture in healthy, anesthetised, spontaneously breathing horses. Under such conditions, the indices should not be used to estimate pulmonary dead space.

Ethics Statement

This experimental study was carried out in accordance with the recommendations of the ARRIVE guidelines and had ethical approval by the local committee for animal experimentation of the Swiss government (TV-4985).

Author Contributions

All authors contributed to conception and design of research. MM, UA, and RB performed experiments. AR, SB, GH, GT, and JS analyzed data and did statistical analysis. All authors interpreted results. MM, SB, AR, GT, and JS drafted manuscript. All authors edited, revised, and approved final version of the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Funding

The want to thank the “Stiftung Forschung für das Pferd” for financing this project (Grant number: PR 2012/02).

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