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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Vet. Sci. | doi: 10.3389/fvets.2019.00399

Brazilian green propolis as a therapeutic agent for the post-surgical treatment of caseous lymphadenitis in sheep

 Mauricio A. Kalil1, Laerte M. Santos1,  Thiago D. Barral1,  Daniela M. Rodrigues1, Neila P. Pereira2, Marcelo A. Umsza-Guez3, Bruna A. Machado4, Roberto Meyer1 and  Ricardo W. Portela1*
  • 1Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Brazil
  • 2Faculty of Pharmacy, Federal University of Bahia (UFBA), Brazil
  • 3Health Sciences Institute, Federal University of Bahia, Brazil
  • 4Health Technology Institute, SENAI Cimatec, Brazil

As antibiotics are ineffective when used to treat caseous lymphadenitis, the surgical excision of lesions is often required. Iodine solution (10%) is currently the choice for the post-surgical treatment; however, it may cause histotoxicity. Propolis are resinous substances composed by a mixture of different plants parts and molecules secreted by bees. As green propolis has already proven to possess antibacterial and wound healing properties, this study aimed to evaluate the use of a green propolis-based ointment as a therapeutic agent for the post-surgical treatment of caseous lymphadenitis. The caseous lesions of 28 sheep were surgically excised before dividing animals into two groups: (1) iodine-treated animals and (2) sheep treated with an ointment made with a previously characterized green propolis extract. Clinical data of animals, size of the scar area, the presence of moisture and secretion in the surgical wound, the humoral immune response against the bacterium and the susceptibility of C. pseudotuberculosis clinical isolates to the green propolis extract were analyzed. The green propolis-treated group presented complete healing of the surgical wound one week before the iodine-treated group. Additionally, animals treated with the green propolis ointment had fewer cases of wound secretion, but it was not statistically different from the iodine-treated group. No clinical signs indicating green propolis toxicity or other side effects were found, associated with a faster and more organized hair recovery by propolis use. The green propolis extract was able to inhibit the growth of 23 from the 27 C. pseudotuberculosis clinical isolates, with minimum inhibitory and minimum bactericide concentrations ranging from 01-08 mg/mL, and did not interfere with the humoral immune response against the bacterium. In addition, green propolis was able to inhibit biofilm formation by four of the C. pseudotuberculosis clinical isolates. We concluded that green propolis is a promising therapeutic agent to be used in the post-surgical treatment of caseous lymphadenitis in small ruminants due to its effects on surgical wound healing, hair recovery, inhibition of wound contamination and bacterial growth.

Keywords: antimicrobials, Corynebacterium pseudotuberculosis, Sheep, Wound Healing, Natural Products

Received: 26 Aug 2019; Accepted: 28 Oct 2019.

Copyright: © 2019 Kalil, Santos, Barral, Rodrigues, Pereira, Umsza-Guez, Machado, Meyer and Portela. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: DVM, PhD. Ricardo W. Portela, Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil, rwportela@gmail.com