Original Research ARTICLE
Evaluation of an indirect ELISA using recombinant arginine kinase for serodiagnosis of Psoroptes ovis var. cuniculi infestation in rabbit
- 1Sichuan Agricultural University, China
- 2Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, China
Psoroptes ovis var. cuniculi is a common ectoparasite of the wild and domestic rabbits worldwide, which causes significant economic losses in commercial rabbit breeding. In China, the diagnosis of rabbits infested with P. ovis var. cuniculi currently relies on detection of clinical signs and Psoroptes mites in skin scrapings by microscopy examination. However, this method is not very efficient in detection of the low mite loads and/or sub-clinical infections. In the present study, we cloned and expressed an arginine kinase homologue gene from P. ovis var. cuniculi (Poc-AK) and used its recombinant protein rPoc-AK to develop an indirect enzyme-linked immunosorbent assay (iELISA) method for diagnosis of P. ovis var. cuniculi infestation in rabbits. The results showed that the rPoc-AK protein was ~ 61 kDa and had no signal peptide. The rPoc-AK-based iELISA achieved a 94.4% sensitivity and a 88.2% specificity, and was able to detect P. ovis var. cuniculi infection as early as the 1st week post-infection, prior to the appearance of clinical signs. Further field study showed 24.94% (66.3/266) clinically normal rabbits were seropositive with the highest and lowest seropositive rates for California (35.71%) and Belgian (15.14%), respectively. These results suggested that the rPoc-AK has potential as a diagnostic antigen for early P. ovis var. cuniculi infestation in rabbits.
Keywords: Psoroptes ovis var cuniculi, Arginine Kinase, Indirect ELISA, Early serodiagnosis, rabbit
Received: 26 Aug 2019;
Accepted: 05 Nov 2019.
Copyright: © 2019 Gu, Gu, Ren, Zheng, Yang, Zhou and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Mx. Xiaobin Gu, Sichuan Agricultural University, Ya'an, China, firstname.lastname@example.org
Mx. Yue Xie, Sichuan Agricultural University, Ya'an, China, email@example.com