Constitutive activity of the inhibitory G protein pathway mediated by non-visual opsin Opn7b reduces cFos activity in stress and fear circuits and modulates avoidance behavior

Stefan Herlitze^1*Hanna Böke¹Hannah Schulte¹Maria Worm¹Julia Bihorac²Brix Mücher¹Martin Hadamitzky²Ida Siveke^1,3Katharina Spoida¹

• ¹Ruhr University Bochum, Bochum, Germany
• ²Institute of Medical Psychology and Behavioral Immunobiology, University Hospital Essen, Essen, North Rhine-Westphalia, Germany
• ³Bridge Institute of Experimental Tumor Therapy, University Hospital Essen, Essen, Germany

Constitutive activity of G protein-coupled receptors (GPCRs) plays an important role in brain function and disease including neurodegenerative and psychiatric disorders. The non-visual opsin Opn7b is a constitutively active Gi/o coupled GPCR which has been used to synchronize neuronal networks. Here we show that expression of Opn7b in the bed nucleus of the stria terminalis and the ventral tegmental area, two interconnected brain areas involved in modulating fear and stress responses, reduces the number of cFos positive neurons and modulates avoidance behavior in mice. Thus, by constitutively activating the Gi/o pathway Opn7b can be used as a tool to reduce cFos expression and to link cFos-expressing neurons to network-and pathway-specific behavior.