Abstract
The recent pandemic has led to the fabrication of new nucleic acid sensors that can detect infinitesimal limits immediately and effectively. Therefore, various techniques have been demonstrated using low-dimensional materials that exhibit ultrahigh detection and accuracy. Numerous detection approaches have been reported, and new methods for impulse sensing are being explored. All ongoing research converges at one unique point, that is, an impetus: the enhanced limit of detection of sensors. There are several reviews on the detection of viruses and other proteins related to disease control point of care; however, to the best of our knowledge, none summarizes the various nucleotide sensors and describes their limits of detection and mechanisms. To understand the far-reaching impact of this discipline, we briefly discussed conventional and nanomaterial-based sensors, and then proposed the feature prospects of these devices. Two types of sensing mechanisms were further divided into their sub-branches: polymerase chain reaction and photospectrometric-based sensors. The nanomaterial-based sensor was further subdivided into optical and electrical sensors. The optical sensors included fluorescence (FL), surface plasmon resonance (SPR), colorimetric, and surface-enhanced Raman scattering (SERS), while electrical sensors included electrochemical luminescence (ECL), microfluidic chip, and field-effect transistor (FET). A synopsis of sensing materials, mechanisms, detection limits, and ranges has been provided. The sensing mechanism and materials used were discussed for each category in terms of length, collectively forming a fusing platform to highlight the ultrahigh detection technique of nucleotide sensors. We discussed potential trends in improving the fabrication of nucleotide nanosensors based on low-dimensional materials. In this area, particular aspects, including sensitivity, detection mechanism, stability, and challenges, were addressed. The optimization of the sensing performance and selection of the best sensor were concluded. Recent trends in the atomic-scale simulation of the development of Deoxyribonucleic acid (DNA) sensors using 2D materials were highlighted. A critical overview of the challenges and opportunities of deoxyribonucleic acid sensors was explored, and progress made in deoxyribonucleic acid detection over the past decade with a family of deoxyribonucleic acid sensors was described. Areas in which further research is needed were included in the future scope.
1 Introduction
Deoxyribonucleic acid (DNA) carries the genetic information constituent of deoxyribose and nitrogenous bases known as nucleotides or fragments of DNA (Wilkins, 1956). These nucleotides contain genetic information that can encode life (Stegmann, 2005). As its size is comparable to the nanoscale, the double helix structure was reported in 1953 by Watson and Crick (1953). This well-known DNA double helix is formed from pairs of complementary single-stranded DNA (ssDNA). Double-stranded DNA (dsDNA) is a pair of bonded ssDNA (Watson and Crick, 1953).
Structural nanotechnology has made significant progress in terms of the rapid sensitivity of DNA sequences (Zhou et al., 2020). Each existing DNA is unique, indicating that DNA sequence is crucial for detecting, and it has been studied in the fight against sporadic pandemics. A small part of the DNA that holds genetic information is known as a gene. A complete DNA sequence of DNA is imperative for the fabrication of vaccines. Over the past two decades, many new methodologies have been developed for the detection of DNA, which has ultimately assisted in portable point-of-care diagnostics. Indigenous DNA is negatively charged (Zhang et al., 2007), which can be manipulated by several strategies, such as creating an electric field; thus, it behaves like electrons to attract positively charged particles (Liu and Hu, 2007). Therefore, the identification of specific DNA sequences is a crucial task achieved using modern nanotechnology, which consequently opens a new era of exploration ranging from detection to gene mutation. DNA sensors are capable of detecting changes in the form of electrical signals generated through the immune system in response to any perturbation. They convert biochemical reactions into a signal for further detection. Currently, integrated multiscale simulation and experimental techniques are used to study biosensing applications in a variety of interdisciplinary fields (Quan et al., 2018; Mohammadi et al., 2021; ).
Low-dimensional (LD) materials are a class of materials with extraordinary characteristics (). They include graphene and carbon nanotubes (CNT), MXenes (Ti2C3), hexagonal boron nitride (h-BN), molybdenum disulfide (MoS2), and reduced graphene oxide (rGO), etc. (Mohammadi et al., 2021). They have displayed they are being used in a variety of applications. Currently, LD based sensors are replacing traditional sensors (Wu et al., 2022). LD materials are used for protective coating and biosensors due to their tunable, electrical, and optical, and excellent mechanical properties. They are also being used as substrate materials in electronic sensors due to their multilayered structures. Additionally, these materials modify their surface chemistry through associated functional groups due to which they respond to a specific analyte (). Other than normal 2D structures, very new types of nanostructured materials with improved optical properties have been reported (Kim and Benelmekki, 2019). They have diverse industrial applications, particularly suitable for biomolecular sensing. These are known as “smart nanosheets or nanoscrolls” (Kim et al., 2014; Kim and Benelmekki, 2018; Kim and Benelmekki, 2019;).
To achieve global market demand, the fabrication of DNA sensors should possess a low cost, low range of detection (LOD), simple and low processing time with high speed, and high selectivity. There are two main types of DNA detection techniques: conventional spectrophotometric and polymerase chain reactions (PCRs), and modern nanomaterial-based sensors, which are further subdivided into optical and electrical sensors. Optical sensors based on fluorescence (FL), surface plasmon resonance (SPR), colorimetric, and surface-enhanced Raman scattering (SERS) techniques, whereas electrical sensors based on electrochemical luminescence (ECL), chip-based, and field-effect transistors (FET) are described in Figure 1.
FIGURE 1
Detection methods based on electrical sensors have been gaining attention in the global market owing to their high multiplexing capability, high sensitivity, and wide dynamic range. The major contribution of biosensor involves a transduction mechanism for detection. The transducer transforms the electrical signal from the analyte and amplify it. Conventional sensors have low accuracy and expensive and complex instrumentation whereas nanomaterial-based sensors are more compatible and provide a proficient detection. However, the optical method is commonly used because it has numerous defects, such as it is difficult to reliably profile low-abundance genes, and it requires expensive fabrication of these optical devices and complex bioinformatics tools for fluorescence signal identification. FET-based nano electronic devices have attracted much attention owing to their small scale, simple design, and better performance compared with conventional devices.
Because of the discovery of DNA sensors, efforts have been made to demonstrate and optimize them for portable point-of-care applications. The continuous demand and advancement of nanotechnology has been pointed out by experts, and the DNA sensor market is predicted to reach up to 28 billion dollars. This set an annual growth rate of 8.4% in 2022, as illustrated in Figure 2. In particular, Figure 2 represents data rooted from Scopus using search title as “DNA/RNA/Nucleic acid sensor” and immunosensor/antibody sensor, and “Enzyme’’ sensor (Mujawar et al., 2020).
FIGURE 2
2 DNA sensors and types
A sensor is a combination of receptor-transducer, which converts the biochemical response to signals emitted from the source. Moreover, DNA sensors monitor involving biomolecular processes. A biosensor consists of an analyte, bioreceptor, transducer, electronics, and display setup (Naresh and Lee, 2021).
2.1 Conventional sensors (Cs)
2.1.1 Spectrophotometric sensor (Sps)
The interaction of light with matter, known as spectrometry, is an ancient technique that has been used in the fields of chemistry and biomedicine because of its low cost, simplicity, and convenience. This has led to the analytical detection of DNA derivatives, such as nucleic acids and nucleotides, owing to the presence of double bond systems as a consequence of these bonds responding to ultraviolet light in the spectrum. This type of specific absorbance will be helpful for the quantitative measurement of DNA by spectrophotometry in the form of micrograms/mL. The absorbance phenomenon corresponds to the transition of electrons from either the anti-bonding (π*) state to the non-bonding (n) state or from the anti-bonding (π*) state to the bonding state (π) associated with a specific energy (Mujawar et al., 2020). Infect nucleic acids have maximum absorbance of UV light at a wavelength of 260 nm; thus, a solution of DNA is exposed to quantify the exposed sample. The drawback of this methodology is that there is a considerably weak signal for single-stranded nucleic acids owing to the lack of bonding, anti-bonding, and non-bonding states. Furthermore, this technique cannot describe the detailed sequences of nucleic acids. As nucleic acids absorb UV light at 260 nm, the compound containing them absorbs UV at 280 nm. The purity of the sample can be calculated by the ratio of absorbance measurement following the criteria for being pure, i.e., (Shen, 2019).
2.1.2 Polymerase chain reaction-based sensor (PCRs)
Polymerase chain reaction (PCR) is an in vitro technique that can detect and amplify small DNA segments. Through this technique, millions of copies are developed in a fraction of the time. This method was proposed in early 1980 (), and has been used globally in various fields, such as food quality insurance, genetics, molecular biology, and forensic science. It is a well-established amplification technique during which millions of amplitudes are generated from a small segment of DNA in a short interval of time. Although it is a low-cost and universally used method, constraints, such as indirect melting temperature measurements and premier annealing, hinder their applications. Furthermore, specific amplification will only occur within a narrow range of reaction conditions that ultimately cause the failure of the PCR testing methodology.
For the sample preparation, errors in the volume of reagents significantly alter the result of the polymerase chain reactions cycle that may disrupt the melting temperature, premier annealing, and amplification. All of the building blocks of PCR hinder portability, as these types of measurements cannot be performed outside the laboratory in a well-controlled environment. Signal transduction mechanisms are another significant barrier that prevents PCR from becoming a popular portable device without the use of expensive instruments, such as PCR machines, and complicated processes, including electrophoresis. All these challenges limit the use of PCR as a portable device. Therefore, a simple modification was introduced using a colorimetric assay for the reserved transcription of DNA fragments known as real-time PCR (rt-PCR). RT-PCR was first described in 1990, and a schematic description is shown in Figure 3, which displays a comparison of the different PCR techniques. Conventional PCR, also known as end-point PCR for analysis of DNA amplification, was conducted at the end of fluorescent marking, whereas amplified DNA was analyzed during each cycle, also known as real-time PCR (Wang et al., 2018b). In general PCR, the fragmentation of DNA is pulled through the gel matrix using a centrifugal electric field that separates DNA segments. This process is called electrophoresis. The second PCR test known as qPCR is quantitative PCR, and it is a much more dynamic range of analysis than that of conventional PCR. It is also a modified form of PCR to qualitatively analyze the DNA by the introduction of florescent dyes during PCR cycles. The output of rPCR is typically displayed in the form of sinusoidal followed by a plateau. Digital PCR (dPCR) follows the random distribution of particles over numerous partitions. Each partition acts as an individual PCR through fluorescence detection. Poisson’s statistics were applied to the sample partitions to calculate the concentration of the target sequence from the proportion of the amplified positive concentration. The process constitutes independent partitioning and amplification followed by florescence detection.
FIGURE 3
2.2 Nanomaterial’s base sensors (NMBs)
Nanomaterial-based sensors are considered efficient sensors with excellent signal absorption strength due to quantum effects. They have a high surface-to-volume ratio and higher optical and magnetic properties which make them reasonably good for sensing all types of analytes (Santhanam et al., 2020). Numerous NMBs have been used for DNA biocompatibility because of their higher signal detection capability and transduction technology which converts signals from an analyte during its biochemical reaction (Hlongwane et al., 2019). DNA sensing using NMBs has been included in the following sections.
2.2.1 Optical sensors (Ops)
These biosensors measure changes in optical properties, such as resonance, reflectance, absorbance, and luminescence from the sensor surface. This system measures the fluorescence from nanomaterials based on the detection of DNA hybridization (Vikrant et al., 2019). The optical sensors have been attracted due to their efficient detection level. The materials used in these detectors effectively amplify the detection signal. We summarized a few optical techniques used for developing nanomaterial-based DNA biosensors.
2.2.1.1 Fluorescence-based DNA sensors (FBs)
In such sensors, a fluorescent nanomaterial, known as the transducer, is conjugated with a DNA molecule acting as a target molecule. A fluorescence spectrophotometer was used to measure the fluorescence emitted from the nanomaterial conjugated with the target DNA, which is known as fluorescence resonance energy transfer (FRET) (Ploetz et al., 2016). In this technique, excited electrons were transferred from DNA to nanomaterials at resonance separated by a nanoscale distance without emission of a photon; this process is called fluorescence quenching (FQ). In the presence of the probe and target DNA, both combine to form a hybrid that leads to a change in the fluorescence intensity of the nanomaterials, similar to the aptamer/DNA sensor. Figure 4 shows a scheme of target detection based on FRET with reduced graphene quantum dots (GQDs) combined with probe (complementary) DNA (single-stranded). A spectrophotometer was used to measure the resonance intensity at the graphene monolayer surface. The FRET signal emerging from the layer was analyzed for the DNA structures. Initially, inorganic GQDs reaction with NaBH4 (sodium borohydride) results in reduced GQDs called (rGQDs) followed by connecting DNA (cDNA). This reaction is further divided into three steps. In the first step single strand DNA (ssDNA) interact with GQDs via a condensation reaction. Later, this interaction is absorbed through electrostatic stacking making a base pair of ssDNA-rGQDs/GO. Lastly, the base pair interact with the target DNA (tDNA). This tDNA is replaced by a single-based mismatched DNA (mDNA) making a double-strand DNA (dsDNA)-rGQDs compound detached to produce florescence recovery (Qian et al., 2014).
FIGURE 4
An overview of the variety of recently reported FRET-based DNA detection techniques is listed in Table 1. This table shows the complete information reading type of materials used, techniques and strategies of DNA detection, limit of detection, and range of analyte concentration. Herein, we can conclude that by using a tetrahedral DNA framework, we can achieve a DNA detection limit of 1 fM (Li et al., 2020b) while using Cu (I)-catalyzed alkyne-azide cycloaddition (CuAAC) as a fluorescent nanomaterial can achieve a detection range of 0.22 fM reported by Zheng et al. (2020). Furthermore, Zhang D et al. (2020) reported a 23.8 aM level detection limit using the composition of magnetic beads (MBs) that contain phosphate- Zr4+-carboxylate/Cu(II) Br/EDTA for lung cancer DNA. Another finding of magnetic nanoparticle-based on poly-enzyme nanobeads fluorescence assay show high detection of 1.6 aM, which is the highest ever reported by FRET techniques (Lapitan et al., 2019).
TABLE 1
| Type of Affinity Assay | Techniques/Strategy | LOD | Analyte Range DLR | References |
|---|---|---|---|---|
| MoS2 | Fluorescence/van der Waals force b/w DNA and MoS2 in quenching | 0.0039 U/mL | 0.0039 U/mL | Zhao et al. (2020) |
| AuNP-H1 probes | Fluorescence/Dual signal amplification | 47.68 fM | 50 fM–100 pM | Zhang et al. (2020d) |
| Copper Nanocluster | Fluorescence/was quenched b/w Copper and aptamer | 4.8 ng/L/L | 0.01–1,000 ug | Zhao et al. (2020) |
| TAE/Mg+2 buffer | Fluorescence/isothermal signal amplification | 9.8 pM | 0.01–10 nM | Zhao et al. (2020) |
| Silica Nanoparticles | Fluorescence/polydopamine modified SiO2 as quenchers | 1 nM | 0–12 nM | Zhao et al. (2020) |
| δ-FeOOH nanosheet | Fluorescence/DNA and FeOOH as quenchers | 10 pM | 0–20 nM | Wu et al. (2020a) |
| Curcumin Encapsulated with F108 | hydrogen bonding and dipole interaction between curcumin and F108 increasing FL to 6 times | 50 uM | 0–100 μM | |
| DNA Tetrahedron Nanoprobe | FRET/DNA assisted cyclic amplification | 6 pM | 10 pM–100 nM | |
| Tetrahedral DNA Framework | Fluorescence/developed bridge DNA sensors that can capture target DNA | 1 fM | 0.01 nM–10 nM | Li et al. (2020b) |
| Carbon Nanoparticles | Fluorescence/developed DNase I-aided cyclic enzymatic amplification method | 3.2 pM | 25–100 pM | Li et al. (2020d) |
| Plasmonic gold nanostars | SERS/Fluorescence/developed a dual-modal aptamer-based biosensor | FL: 0.50 μg/L and SERS: 0.77 μg/L | 0.1–50 μg/L | Li et al. (2020d) |
| Poly- nucleotide kinase | Fluorescence/isothermal signal amplification | 0.1–0.005 U/mL | 3 uU/mL | Li et al. (2020e) |
| Cy5/Fluorescein | FRET/developed a ratiometric fluorescence method for Four-way Junction | 0.12 nM | 1–200 nM | Liu et al. (2020b) |
| G-triplex micro beacons/thioflavin | Fluorescence/Label-free fluorescence method combined G3-MB with Exo III‒aided DNA recycling amplification | 5.6 pg/mL | 75–750 nM | Liu et al. (2020c) |
| GO based modified with Cu(II) | PL/graphene oxide-based modified platform that boost photoluminescence | 4.6 × 10–7 M | 0–20 μM | Pal et al. (2020) |
| Thioflavin/ErBr | FRET/ThT-fluorescence-based DNA composition studied | 1.1 nM | 0–4uM | Pramanik et al. (2020) |
| Carbon Dots and Au Nanoparticles | Fluorescence/A FQ energy transfer as “spectroscopic rulers” between CDs and AuNPs | 1.03 ± 3.54 nM | 0.01–200 nM | Saad et al. (2020) |
| Biotinylated sensing probes/magnetic beads | Fluorescence/DNA methyltransferase catalyzes and produce strong fluorescent signalssignals | 0.002 U/mL | 0.01–10 U/mL | Liu et al. (2020f) |
| Au nanoparticles on covalent organic framework nanosheets | Fluorescence/Au NPs/COF NSs used as an efficient quencher | 75 pM | 0.1–10 nM | Tian et al. (2020) |
| Fe3O4 MBs/FL | Fluorescence/activation of a hybridization sensor by a magnetic field | 2-fold | , | |
| Fe3O4/SiO2/Graphene | FRET/graphene oxide-based FQ sensor. (fluorescent magnetic nanoparticles as donor) | 0.12 µM | 0–10 µM | |
| multicolor QDs open-ring nanoarrays with silver-Plosmonic | FRET/Fluorescence enhancement and quenching can be switched that provide platform for DNA detection | ∼300 fM | 100 fM–1 μM | Kannegulla et al., 2018a, Kannegulla et al., 2018b |
| Plane Silver - 6 nM | ||||
| MBs/phosphate-Zr4+-carboxylate/Cu(II)Br/EDTA | Fluorescence/approach used electron transfer atom transfer radical polymerization with EDTA as the metal ligand | 23.8 aM | 0.1 fM–1 nM | Zhang J et al. (2020) |
| Cu (I)-catalyzed alkyne-azide cycloaddition | Fluorescence/Based on controllability and signal amplification by atom transfer radical polymerization | 0.22 fM | 1 fM–1 nM | Zheng et al. (2020) |
| MoS2, WS2, and GO | Fluorescence/fluorescently labeled DNA oligonucleotides were used and their adsorption capacities and kinetics were studied as a function of ionic strength | WS2-3nM, MoS2-1.5 nM, GO-2nM | 1–50 nM | Lu et al. (2017) |
| Streptavidin-coated magnetic beads/Liposome/carboxyfluorescein | Fluorescent/carboxyfluorescein-loaded liposomes as signal amplification systems strategy for detection of DNA sequence | 1 nM | 1 × 10−10–6×10-10 M | Sforzi et al. (2020) |
| PDMS chip and a glass substrate Microfluidic | Fluorescence/Microfluidic Exponential Rolling Circle Amplification platform | 5–8 (zeptomole) 2 × 106 (exosomes) | 50 zmol to 5 fmol | |
| Polydimethylsiloxane microfluidic device Microfluidic | fluorescence/Develop An ion concentration polarization based electrokinetic concentration device | 1 nM (DNA) | ||
| 25 nM (RMNA) | ||||
| Microfluidic chip/microbead/Microfluidic | fluorescence/developed a rapidly adaptable platform to assess biomarkers using a microfluidic technology | 0.5 ng/μL (chip), 0.1 ng/μL (multiwell plate) | 0.2 ng/μL–100 ng/μL | |
| Poly-L-Lysine Microfluidic chip/ | fluorescence Proposed PLL substrate on with microfluidic chips for detection of DNA based on three-segment hybridizationhybridization | 1 pM/30 min | 10−7M to 10−12 M | |
| LOC for free-flow electrophoretic/ | Fluorescence/demonstrate DNA amplification by microfluidic method from dilute specimens | 1 PFU/mL | 5–500 µL | Hügle et al. (2020) |
| Fe3O4@PDA NPs by Ca2+ | FRET/developed FRET-based biosensor for determination of microRNA-167 using CDs as donor and Fe3O4@PDA NPs as acceptor | 76 pM | 0.5–100 nM | |
| Iron oxide nanocubes microfluidic | Flow cytometry/Designed a new kind of superparamagnetic nanobeacon for mRNA detection and regulation in living cells | 9.6-fold | 54 nM | |
| Magnetic nanoparticle/poly-enzyme nanobead/FL | Detection target DNA by combining magnetic nanoparticle capture and poly-enzyme nanobead signal amplification | 1.6 aM | 1 aM–1 pM | Lapitan et al. (2019) |
Overview of FRET-based DNA detection techniques.
2.2.1.2 Surface plasmon resonance-based DNA sensors (SPRs)
It is one of the most common optical sensing techniques which is based on surface plasmon which are electromagnetic waves originating from the metal interface. In this sensor, the incident light stimulates the resonance of the conduction electrons at the interface of the positive and negative permittivity materials. AuNPs with positive charge conjugate with the target DNA that is negatively charged; light stimulus resonance phenomena occur between them using conduction electrons, and there is a change in dielectric constants that generate surface plasmons. The schematic diagram shows the detection method by SPR, where the prism, transducer surface composed of nanomaterials, plane polarized light as a stimulus, and detector are the main components of the SPR system. During the conversion of the association phase to the disassociation phase, the refractive index changes, which ultimately deviate from the exiting light from the prism, are correlated with the concentration of the analyte, as shown in Figure 5. Specifically, Figure 5 represents the experimental illustration of SPR techniques (A) and a variation in the critical angle as a function of the intensity (B) and the response of the sensor during the experiment. This method offers label-free techniques; however, there is variation in the refractive index owing to changes in the transducer surface temperature and composition, which may significantly alter the detection results (Patching, 2014).
FIGURE 5
The SPR detection performs the analysis of biomolecular interaction ranging from organic compounds to proteins and nucleic acids and viruses based on real-time. The detection is non-invasive. i.e., it can analyze transparent or colored samples effectively. They are a label-free, specific, and sensitive method that is dependent on the changes in the refractive index of the material surface. A small change in the refractive index may induce a false signal during the detection of an analyte. Nanomaterials are the most ideal material for their fabrication and better signal detection and amplification. Most importantly they are available commercially (; ).
An overview of the various SPR-based DNA detection techniques reported recently is displayed in Table 2. We have listed all possible information, for example, the type of materials, techniques, and strategy of DNA detection used, limit of detection, and range of analyte concentration. Therefore, by employing a sandwich-like assay based on the selective capture of specific DNA targets, subsequent signal amplification can be obtained by a secondary DNA probe linked to Au nanostars with a detection limit of 3 fM to 6.9 aM (Mariani et al., 2015). Another study reported that a highly sensitive polarization control-modulated plasmonic biosensor based on monolayer graphene with Au film -staking achieved a detection limit of 500 aM (Sun et al., 2019b), which is the highest ever reported for SPR techniques. Table 2 summarizes the findings based on the SPR-DNA sensor reported previously.
TABLE 2
| Type of Affinity Assay | Determination Method | LOD | Analyte Range DLR | References |
|---|---|---|---|---|
| Silica core/gold nanowires | SPR/a novel design of a highly sensitive surface plasmon photonic crystal fiber biosensor for DNA hybridization detection is presented | 5.53–6.82 RIU-1 | 94.59 nm/RIU | |
| Au/SiO2/H2O/L inker | SPR/A highly sensitive hybrid plasmonic slot-waveguide biosensor based on silicon-on-insulator is proposed which record change in the analyte refractive index | 2.65 × 10−6 RIU | 1890.4 nm/RIU | |
| Biotinylated thiolated DNA molecular beacon (MB)/streptavidin functionalized gold nanorods | SPR/strategy by applying biotinylated thiolated molecular beacon (MB) interfacial gene probe and a strepavidinylated GNR (Stre-GNR) for the enhanced SPR response to realize rapid and sensitive miRNA detection is demonstrated | 0.045 pM | 0–2 nM | |
| Nanobowled Aluminum/Au NPs | LSPR/fabricated ordered gold nanoparticle arrangements on epoxy substrates is presented | 5 nM | 1–1,000 nM | Lednický and Bonyár (2020) |
| Gold nanostar (AuNS)/SPR | SPRi/Designed sandwich-like assay based on the selective capturing of specific DNA targets and the subsequent signal amplification by a secondary DNA probe linked to AuNS | 3.0 fM (without NSs) 6.9 aM (with NSs) | 6.1 nM–10 pM (without NSs) | Mariani et al. (2015) |
| 1.5 fM–10 aM (with NSs) | ||||
| 3D gold nanostructure with Au deposition | LSPR of gold nanoparticles is sensitive to the dielectric constant of the surrounding environment | 1–500 nM | 13 fM | Na et al. (2018) |
| Au nanoparticles | LSPR peak is sensitive only to the refractive index of the close surrounding environment | 430 nm/RIU | 0–100 nM | Qi and Bi (2019) |
| Graphene/Au NPs | SPR/Sensitive polarization control-modulated plasmonic biosensor based on monolayer graphene at gold film- -staking interaction | 500 aM | 10−15 to 10−7 M | Sun et al. (2019b) |
| Gold nanorods | Using LSPR/establish a method that can discriminate between the mutant and the wild-type sequence of the gene using gold nanorods in solution | 2 ng/mL | 0–125 ng/mL | Tadimety et al. (2019) |
| Inverted-bowtie nanoapertures | SPR/show simultaneous ionic-current and optical-transmission-based detection of DNA | 100 nm | Verschueren et al. (2019) | |
| Dual Gold Nanoparticles | SPR/Dual nanoparticle amplification was achieved by controlled hybridization attachment of AuNPs resulting from electronic coupling between the Au film and AuNPs, as well as coupling effects in plasmonic nanostructures | 5 × 103 exosomes/mL | 106–109 exosomes/mL | Wang et al. (2019b) |
| Gold NPs | LSPR/AuNPs bind with DNA, this binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the LSPR peak wavelength | 60 nM | 0–31 nM | Zopf et al. (2019) |
| Silicon dioxide Microfluidic | LSPR/developed a label-free microfluidic biosensor platform to detect the interaction of DNA with the DNA polymerase enzyme, to monitor the formation of Self-assembled-monolayers of ssDNA | 54 ± 6 nm/RIU | 0.0625 U/mL | Roether et al. (2019) |
SPRs based DNA detection techniques.
2.2.1.3 Colorimetric-based DNA sensors (CMs)
In this technique, color tags or enzymes are used for the detection of DNA compatible with the substrate. Alternatively, nucleic acids functionalized with nanomaterial-based assays can be used for the detection and quantification of DNA (Krishnan and Syed, 2022). During colorimetric analysis, colloidal solution gold nanoparticles (GNPs) exhibit different colors based on their distance from red to blue. The detection is done through a change in the wavelength of the electrolyzed DNA. The color of GNPs is dependent on their dispersion and this help researchers to visually investigate assays that change owing to a decrease in the average distance between nanomaterials (Li and Rothberg, 2004). This method is straightforward, low-cost, and easy to perform for rapid onsite diagnostics. They have a poor limit of detection and prototype design. This DNA detection equipment is commercially available and more economical than other clinical diagnostics. Figure 6 displays schematic illustration of calorimeter based on the GNPs dispersion and aggregation (Liu et al., 2013). A series of DNA samples were electrolyzed and had different concentrations up to 6Nm. The color of the GNPs is gradually changing from pale to blue with the addition of concentration. The wavelength of absorption spectrum is in the range of 550–750 nm. This method is designed to detect a DNA sequence.
FIGURE 6
An overview of the various colorimetric DNA detection techniques recently reported is listed in Table 3. We summarized the types of materials used, techniques and strategies of DNA detection, limit of detection, and range of analyte concentration. Therefore, by employing electrophoretic streptavidin-coated MBs assisted with a magnetic field, we could introduce a new method, which uses active hybridization with a detection limit of 0.1 fM (Tian et al., 2019). Another report showed that Fe3O4 nanosheets in DNA/Fe3O4 networks display peroxidase-like catalytic activity, thereby enhancing detection to an extreme limit of 13 aM, which is the highest limit that has been reported for colorimetric techniques (Tang et al., 2019).
TABLE 3
| Type of affinity assay | Determination method | LOD | DLR | References (s) |
|---|---|---|---|---|
| 2D DNA/Fe3O4 nanosheet | Colorimetry/Fe3O4 nanosheets in the DNA/Fe3O4 networks display peroxidase-like catalytic activity thereby enhancing detection | 13 aM | 0.05 fM–12 nM | Tang et al. (2019) |
| Fe2O3 porous particles | Calorimetric/DNA is detected using the peroxidase-like activity of a composite nanozyme synthesized in the form of porous particles | 1.1 nM | 0.0–21.5 nM | |
| Magnetic microbeads/ nanoparticles | Optomagnetic/isothermal amplification technique | 4 and 20 pM | 1–100 pM | Minero et al. (2019) |
| Magnetic microbeads | Optomagnetic/isothermal nucleic acid amplification | 4 pM | 2–40 pM | Minero et al. (2020) |
| Iron oxide nanoparticles | Optomagnetic/giant magnetoresistive effect | p = 0.05 | Ng et al. (2019) | |
| Streptavidin-coated MBs/electrophoresis | Electrophoretic assisted with magnetic field/multiplex microarray-based assay/new method of active hybridization analysis | 0.1 fM | 0.1 nM | Shlyapnikov et al. (2019) |
| Magnetic nanoparticle-graphene oxide | Optomagnetic/isothermal nucleic acid amplification triggered by the hybridization and padlock probes | 2 pM | 1–4 pM | Tian et al. (2019) |
| Gold nanoparticles probe | Plasma mass spectrometry detection/Au NPs amplification and isothermal circular strand-displacement polymerization reaction | 8.9 fM | 0.1–10000 pM | Xiao et al. (2019) |
| Silicon master/Microfluidic | Microfluidic recombinase polymerase amplification (RPA) sensor | 10 copies/μL | 10–106 copies/μL | Yang et al. (2019) |
| Peptide Nucleic Acid | PNA-DNA2 triple-helix molecular switch and DiSC2 | 0.18 nM | 0–2 μM | Xu et al. (2020a) |
Colorimetric-based DNA detection techniques.
2.2.1.4 Surface-enhanced Raman scattering-based DNA sensors (SERSs)
In this technique, molecular vibrations which arise directly from analyte molecules were measured using Raman spectroscopy. Such types of resonance occur only when the target analyte is a nanostructure or a roughed metal surface. SERS sensing is based on the conjugation of nanomaterials and bioreceptor molecules (oligonucleotides) at the surface of a dye known as a Raman reporter or tag that enhances Raman signals during the detection of target DNA. SERS provides an enhanced Raman signal of 106–1014 order of magnitude owing to the electromagnetic interaction between the metal.
Nanostructures and the analyte. SERS-based biosensors have a comparatively low cost, high sensitivity, rapid results, and portability (Vikrant et al., 2019). Figure 7 shows the schematic setup for the detection of DNA based on AgNPs at the Si substrate as a sandwich-type sensing setup. Nanoparticles are functionalized with thiolated DNA as step 1, followed by conjugation of dye named Rhodamine as step 2. Therefore, a sandwich-like structure surrounded the target DNA by thiolated DNA and reported DNA that ultimately caused the formation of capture/target/reporter DNA ready for SERS detection as step 3, as shown in Figure 7. The optical sensors are summarized in Figure 8. Each optical sensor has been summarized in the flow chart.
FIGURE 7
FIGURE 8
An overview of the various SERS-based DNA detection techniques reported recently is listed in Table 4. It contains the complete information type of materials used, techniques, a strategy of DNA detection, limit of detection, and range of analyte concentration. Thus, a detection limit of 10 uM–10 fM can be achieved by AuNPs on the surface of graphene oxide (GO) linking via hybridization (Khalil et al., 2019). reported a novel detection ratiometric sensor based on glucose oxidase (GOx) on Au and Si nanoflower substrates that enhances the detection to an extreme limit of 7.75 aM, which is the highest limit for colorimetric techniques. Table 4 lists earlier studies on SERS-based DNA sensors.
TABLE 4
| Type of affinity assay | Determination method | LOD | DLR | References(s) |
|---|---|---|---|---|
| DNA Probe-conjugated AuNPs | SERS/Salivary biomarkers S100 calcium binding protein p (S100 P) mRNA in saliva is a potential biomarker | 3 nM | 0–200 nM | |
| Au NPs probe | SERS/Colorimetry/developed dual-mode Au NPs probe | 1 cell | 100–106 cells mL-1 | |
| Ag NPS/SERS | SERS/The simple strategy relies on the electrostatic adhesion of DNA/RNA onto positively charged silver colloids thus providing realistic direct information of the nucleic acid | 1 pg μ/L | ||
| Polyadenine/AuNS | SERS/Developed a method for the modulation of DNA conformation from the “Lie-Down” to the “Stand-Up” conformation on a AuNS surface by adjusting the length of tip-assembled polyA grafted to the DNA sequence | 45.7 pM | 0.1–500 nM | |
| Silver nanoparticles and Graphene oxide-based biochips | SERS/Laser scribing method to fabricate biochips as a reusable sensor | 10−5–0−10 M | 10−10 M | |
| Au NPs were conjugated with oligonucleotides | SERS/Developed SERS-based vertical flow assay biosensor | 0.01–200 nM | 1.1–10 nM | |
| Glucose oxidase (Gox) on Au & Si nanoflowers substrate | SERS/fabricated ratiometric sensor with only one Raman probe based on cascade catalytic reaction | 7.75 aM | 10 aM–100 pM | |
| DNA on the AgNPs@Si | SERS/AgNPs@Si based substrates for sensitive,specific,andmultiplexDNA detection | 1 pM–100 nm | 1 pM | Jiang et al. (2012) |
| GO-Au NPs/Raman dye | SERS/detection is based on the covalent linking of the two platforms [GO-AuNPs with probe 1 and AuNPs with probe 2 and Raman dye (Cy3) ] via hybridization | 10 µM–10 fM | 10 fM | Khalil et al. (2019) |
| Metal–organic framework coated Ag-NOF Sensor | SERS/reported that a MOF enables an Ag nanowire SERS platform to be corrosion resistant | 1 nM | ||
| Au–Ag bimetallic nanodendrites | Demonstrated SERS-based sensor that utilizes the toehold-mediated DNA displacement reaction as a target-capturing scheme | 200 fM–20 nM | 96.3 fM | Hyejeong Jang (2019) |
| GaN/Au Substrate/SERS | SERS/Detection of gene mutation-using highly active and reproducible substrate (photo-etched GaN covered with a thin layer of sputtered gold) | 6.75 pg μ/L −67.5 ng μ/L | 1 pg μ/L | Kowalczyk et al. (2019) |
| R6G + AgAu alloy + silicon microbead | SERS/proposed strategy by combining stable SERS reporter element and duplex-specific assisted signal amplification for quantitative detection | 12 fM–18 pM | 5 fM | Ma et al. (2018) |
| PDMS chips integrating silver-coated porous silicon membranes | SERS/metal–dielectric nanostructures were functionalized with enzyme-linked immunosorbent assay for the detection of mRNA. | 25–1 nM | 0.55–1.51 nM | Novara et al. (2017) |
| Graphene-Ag array | SERS/developed graphene-Ag array for the detection of methylated DNA and its oxidation derivatives | 200 pg genomic DNA | 1.8 pmol/L | Ouyang et al. (2017) |
| DNA hydrogel/SERS | SERS developed a novel sensor array with nine sensor units that can detect multiple miRNAs in one sample based on a target miRNA-responsive DNA hydrogel | 4–1,200 nM | 0.11 nM | Si et al. (2020) |
| Silver-coated gold nanostar | SERS/in vivo detection of nucleic acid involving the “inverse molecular sentinel” detection scheme using plasmonics-active nanostars | 5 nM | Wang et al. (2018a) | |
| Co/Au NPs | SERS/PCR/based new platform was proposed and evaluated its performance by sequentially measuring the Raman signals of DNA after the completion of different thermocycling numbers | 0.1–1,000 pM | 960 nM | Wu et al. (2020b) |
| Melamine resin/Ag/SiO2 nanoparticles | SERS/FL/dual-mode spectroscopic encoded microspheres system based on the combination of FL and SERS spectra encoding was designed for specific DNA detection | 100 uL | You et al. (2017) | |
| 10–10 mol/L | ||||
| Silica-coated/AuNPs, and Au-coated NPs with DNA probes | SERS/developed a magnetic-capture-based SERS assay for the simultaneous detection of multiple nucleic basis | 120 fM | 120–450 pM | Zhang et al. (2019a) |
| Tetracationic Bis-triarylborane | SER/designed new derivatives to investigate the influence of the linker type on DNA/RNA/protein interactions by fluorimetric titration | 10 nM | 0.5 nM–0.005 nM |
SERS-based DNA detection techniques.
2.2.2 Electrochemical sensors (ECs)
2.2.2.1 Electro-chemiluminescence based DNA sensors (ECLs)
In this technique, chemical luminescence and electrochemical processes are combined and named as electro-chemiluminescence (ECL), which results in the emission of light. This photo emission occurs owing to the excitation and de-excitation of electrons between the ground and excited states stimulated by the electrochemical reaction in solution. Light is emitted because of the transfer of exergonic electrons at the electrode surface. This phenomenon is also known as electrogenerated chemical luminescence. The wavelength of the light emitted from the excited and relaxed states corresponds to the energy gap of the molecules. ssDNA is commonly used as a bioreceptor and printed screen, and pencil graphite glassy carbon, and gold are used as the working electrodes in the assembly of DNA sensors. Figure 9 shows the simple strategy and mechanism of detection. This design comprises a target DNA fragment that hybridizes with MBs, where streptadivine and biotin are used as linkers, and the ruthenium probe is conjugated at the other end (Figure 9A). This magnetic bead-assisted ECL reaction occurs in the presence of tripropylamine, where the ruthenium probe Ru (bpy)32+ amplifies the signal isothermally (Zhou et al., 2014).
FIGURE 9
Table 5 presents an overview of the various ECL-based DNA detection techniques reported in the last decade. Herein, we summarize the detection limit of 19.05 aM for biosensor based on the in situ generation of Cu nanoclusters as luminophores and TiO2 as a coreaction accelerator (Liao et al., 2018). Zhang et al. (2020e) constructed MXene (Ti3C2Tx)-based impedimetric aptasensing nanosheets and iron phthalocyanine quantum dots for an enhanced high detection sensitivity of up to 4.3 aM as compared to the individual component-based ECL technique.
TABLE 5
| Type of affinity assay | Determination method | LOD | DLR | Ref (s) |
|---|---|---|---|---|
| AuNPs/Polyamidine/CuInZnS QDs | SPR/ECL/demonstrated novel SPR enhanced ECL. PDA control the separation length and enhanced ECL response, potential charge transfer and ECL quenching- P55 gene detection | 0.03 nm/L | 1–15 nM/L | Liu et al. (2018) |
| CdS nanocrystals and gold nanoparticles | ECL/we firstly enunciated the presence of synergy effect between the electron and energy transfer in an ECL system involving the CdS NCs and Au NPs for detection of the DNA binding protein | 5pM | 0.015–150 nM | Wang et al. (2013) |
| MoS2 nanosheets/sulfur doped boron nitrogen QDs | ECL/Demonstrated distance-dependent plasmon-enhanced ECL in detail with different length DNA chains | 0.17 pmol/L | 0.5 pM–1 nM | Liu et al. (2020e) |
| Peptide nucleic acid/core-shell Fe3O4-Au nanoparticles/ | EC/miRNA sensing strategy based on the specific affinity using Fe3O4–Au–PNA probe as a new carrier in a solid-state nanopore | 10 nM | 2 nM–50 nM | Wang et al. (2019a) |
| Gold disk electrode/piranha solution | EC/Demonstrated signal amplification strategy based on copper-free click chemistry-mediated cyclic ligation of DNA | 7.7 fM | 25 fM–100 pM | |
| Screen printed carbon electrodes/RGO | EC/Investigated the influence exerted by the concentration of GO dispersion as a modifier for screen printed carbon electrodes on the fabrication of an EC biosensor to detect DNA hybridization | 100 nM | 10 nm to 10 µM | |
| PDA + compounds/N, N-bis (2-(trimethylammoniumiodide) (propylene perylene-3,4,9,10-tetracarboxyldiimide (PDA+) | PEC/EC dual-mode biosensor with cationic decorated multifunctional DNA spheres in situ generated on electrode was proposed for sensitive and accurate detection of mRNA | 0.37 fM/PEC | 0.1 fM–1 nM (for PEC) | |
| 0.67 fM/EC | 2 Fm–500 pM (for EC) | |||
| Ti3C2Tx@FePc QDs | Electrochemical/construct a novel ultrasensitive impedimetric aptasensing system | 4.3 aM | 0.01 fM −10 pM | Zhang et al. (2020e) |
| Ga2Te3-based QD/Cs+/Li+/ | EC/In this study a Ga2Te3-based QD genosensor together with metal ions was developed | 0.4 pg/mL | 0.1–1 ng/mL | |
| Antimonene/functionalized with an oligonucleotide/AuE | SPE/electrochemical/demonstrated that antimonene interacts non-covalently but strongly with oligonucleotides | 28.3 pg/uL | 0–25 ng/uL | |
| AuE/exosome/Probe DNA/hpDNA | EC/DPV- herein a sensitive hybridization chain reaction electrochemical assay was fabricated for the detection of exosomal microRNA-122 | 53 aM | 0.1 fM to 0.1 μM | |
| Carbon Nanotube-Gold Nanoparticle Nanoclusters/ | Electrochemical/biosensor combine the synergistic properties of both CNTs and AuNPs, as a promising signal amplification strategy for DNA detection | 5.2 fM | 0.1 pM–10 nM | |
| AuE/Alumina Slurry | Electrochemical/proposed biosensor based on nest hybridization chain reaction initiated by the hybridization of two dumbbell-shaped DNA units | 3 pM | 5 pM–0.5 nM | Huang et al. (2020) |
| Poly o-cresophthalein complexone film/Glossy Carbon Electrode | EC/fabricated POCF modified electrode was and used as a sensor for the simultaneous detection of adenine and guanine as oxidation peak currents | 0.02 μM | 0.08 μM–200 μM | Jayadevimanoranjitham and Narayanan (2020) |
| Gold E/8-hydroxy-2′-deoxyguanosine | EC/Introduced triple signal amplification strategies were introduced to enhance the sensitivity of 8-OhdG | 24.34 fM | 100 fM–10 nM | Jia et al. (2020) |
| CdTe QDs Carbon ink and solid wax/electrochemical | EC cloth-based DNA sensors are developed based on Carbon ink- and solid wax | 8.74 fM | 20 fM to 5 nM | Jiang et al. (2020a) |
| SiO2/AuNPs) barcode/gold label silver | EC/method based on bio-barcode/gold label silver stain dual amplification is presented | 0.23 fM | 1 fM–10 pM | Jiang et al. (2020b) |
| Nanowires of polypyrrole | EC demonstrate the potential of nanostructured polypyrrole formed by template free as platform for amperometric detection of DNA | 0.36 aM | 1 aM–100 fM | Khoder and Korri-Youssoufi (2020) |
| Cu–Ni@N, B rGO (GO) | EC/Demonstrated electrocatalytic performance of Cu–Ni@N,B rGO toward guanine (G) and adenine (A) oxidation | 0.118 μM | 1–160 μM | Lei et al. (2020) |
| Carboxylate-Zr4+-phosphate/AgNPs | EC/proposed detection based on electrochemically mediated atom transfer radical polymerization and surface-initiated reversible addition-fragmentation chain transfer polymerization cascade polymerization and AgNPs deposition | 0.487 aM | 1 aM–10 pM | Li et al. (2020c) |
| Cu NCs/TiO2/ECL | ECL/biosensor based on in situ generation of Cu NCs as luminophore and TiO2 as coreaction accelerator | 19.05 aM | 100 aM–100 pM | Wang et al. (2019a) |
| PFO polymer dots/ECL | ECL-based biosensor demonstrate OH -dependent ECL emission characteristic that detect mRNA | 12.2 aM | 50 aM–1.0 nM | Liu et al. (2020a) |
| Ferrocene/Au electrodes/Al2O3/electrochemical/DPV | ECL/sensing strategy utilizing cooperative proximity hybridization based on a G-quadruplex probe labeled with the thiol | 2.82 × 10−15M | 1 nM–1 fM | Liu et al. (2020d) |
| PolyA-ODNs/Rolling Motor/GTD/Electrochemical | 3D-ECL/Demonstrated DNA probe bridge act as catalytic center during sensing | 0.17 nM | 0.5 nM–1.5 μM | (Z.Liu et al., 2020) |
| Peptide nucleic acid/poly-L-lysine/ethyl glycol/dibenzocyclooctyne/Quartz Crystal Microbalance | ECL/presented the potential gain in sensitivity by the application of azido-PNA probes clicked to a PLL-OEG-DBCO adhesion layer adsorbed on Si-micropillar substrates at various pitches | (9.0 ± 0.2) pmol/cm2 | Movilli et al. (2020) | |
| 10.6 factor | ||||
| GO-wrapped Au nanostars/glossy Carbon electrodes | EC-DPV/sensing platform inspired by a functional “green” electrochemical reduction pathway | 1 × 10−20 M | 1 × 10−20 M-1x10−12 M | Rahman et al. (2020) |
| Trivalent Mg2+ dependent DNAzymes | Electrochemical Detection based on formation of trivalent DNAzyme junctions through a target-initiated catalytic hairpin assembly approach | 0.46 fM | 1 fM to 1 nM | Ren et al. (2020) |
| Capture Probe mercaptohexanol/hexanedithio monolayer | Electrochemical/designed genosensor based on mixed-self-assembled monolayers as DNA inmobilization system | 10 nM | 5·10–10 to 5·10–8 M | Sánchez-Paniagua et al. (2020) |
| CdTe QDs/Methylene blue-labeled aptamer | EC/PEC/proposed a ratiometric aptasensing strategy based on the dual-detection model with a PEC “signal-on” and an EC “signal-off” | 10 nM | 0.03–100 μM | Shen Z et al. (2020) |
| Nitrogen-doped reduced GO/glassy carbon electrode | Electrochemical/based on N-RGO/GCE sensor demonstrated electrochemical response toward the oxidation of guanineguanine | 1.38 × 10−7 M | 4.14 × 10−7–3.71 × 10−4M | Song et al. (2020a) |
| pencil graphite electrodes | EC/presented enzyme-linked DNA hybridization assay using PeGE to detect target DNA sequences in DNA fragments amplified by PCR | 40 fM | 0–50 ng/uL | Špaček et al. (2020) |
| Ti working electrode with electrodeposited Au nanostructures | EC (DPV) reported on-chip biosensor of DNA hybridization using Au NCs working electrodes | 0.97 fM | 10 fM–1 µM | Tripathy et al. (2019) |
| Nanoparticle gold ink on planar substrates Cyclic Olefin Copolymer (COC) | Electrochemical/Designed inkjet-printing of Au NPs at planar substrates of cyclic olefin copolymer as hybridization signal probe | 60-fold higher | Trotter (2020) | |
| Copper-based metal–organic framework/graphene nanocomposite/GCE | Electrochemical/DPV/Designed Cu-MOF/ERGO/GCE electrode for the detection of guanine and adenine in real samples | 0.02–10 µM 0.005–20 µM | 20–100 µM (for guanine) | Wang et al. (2020) |
| 40–200 µM (for adenine) | ||||
| Beacons ferrocene (Fc)-A1/methylene blue -A2 | Electrochemical/Designed DNA circle capture probe with multiple target recognition domains was anchored at the top of tetrahedron DNA nanostructure | miRNA-21–18.9 aM and miRNA-155–39.6 aM | 0.1 fM–10 nM | Xu et al. (2020b) |
| Ferrocene/graphene | Electrochemical/developed a novel tetraferrocene used as homogeneous sensor probe label that provide a greater signaling potential | 8.2 fM | 20 fM–2 nM | Yin et al. (2020) |
| Hairpin DNAN | Electrochemical/developed a novel sensor via target-induced Cas12a cleaving interfacial hpDNA | 30 pM | 50 Pm–100 nM | Zhang Y et al. (2020) |
| Naphthyl phosphate. Dithiothreitol, dimethylamino propyl carbodiimide | Electrochemical a new electrochemical mmune-DNA sensing platform for DNA Mtase activity assay and inhibitor screening by catalysis-based signal amplification | 0.039 Um/L | 0.05–10 U m/L | Yin et al. (2020) |
| Nanonets of GO/Fe3O4/β-CD/PAMAM-avidin-ALP | Electrochemical DNA detection by using host-guest nanonets of GO/Fe3O4/β-CD NCs as Ab platform and PAMAM-avidin-ALP as signal amplification due to electron transfer | 3.2 pM | 0.01–50 nM | Zhou et al. (2019) |
| Gold coated magnetic nanospheres | Electrochemical/Develop 3D magnetic DNA nanospheres were synthesized and immobilized on a gold stir-bar as encoded probes for miRNA capture and signal amplification | 1.5 fM | 5 fM–2 nM | Shen X et al. (2020) |
| Rethenium tris-(bipyridine)/ECL | ECL/developed sensor for 8-oxodGuo activity assay using spermine conjugated ruthenium tris-(bipyridine) derivative (spermine-Ru) | 1 lesion in 500 DNA bases | 0–4 U/uL | Shen Z et al. (2020) |
| Gold Nanocluster-H2O2 system | ECL/based sensor was fabricated for the quantification of 5 mC, TET1 protein and β-GT activities, as well as inhibitor screening, based on the interaction of chemically excited AuNCs with H2O2 | 3.46 pM | 0.01 Nm–50 nM | Jiang et al. (2018) |
ECL-based DNA detection techniques.
2.2.2.2 Microfluidic/PCR chip-based DNA sensors (MFCs)
Lab-on-a-chip (LOC) is a mini-integrated chip with small sensors in an array with an area of a few square centimeters. They are based on the micro-electrical-mechanical-based technology (MEMS) in the form of an integrated chip. These sensors are composed of a network of microfluidic channels in which the analyte can be manipulated at the microscale level. The compact design of microfluidic chips results in rapid heating and mixing; hence, providing ultrahigh sensitivity and portability for direct analysis of a crime scene (Temiz et al., 2015; ). This will also decrease the amount of analyte as well as reduces cross-contamination in the sealed environment. These systems are designed for single use, which will benefit from the chain of custody and contamination risk. A bandage-like flexible sensor that amplifies the DNA detection signal using microfluidic technology is shown in Figure 10. These sensors can be powered by body heat; thus, they are highly sensitive and portable for on-site detection (). They are widely used for DNA amplification process and accelerate PCR process. They are small in dimension and instant detection and cost-effective instrument.
FIGURE 10
An overview of the various microfluidic chip-based DNA detection techniques reported earlier is listed in Table 6. We can observe that biosensors based on microfluidic chips are designed as power-free chips. They follow the amplification of fluorescence signals after hybridization with laminar flow-assisted dendritic cells and have a detection limit of 0.045–0.45 pM (Kim et al., 2019). Microfluidic-based nucleic acid amplification tests as noise resistant quantitative PCR are used for rapid detection of ultralow-abundance DNA in real biofluids. They have the highest diagnostic limits of 0.05 aM to be reported for a microfluidic technique (Ye and De, 2017).
TABLE 6
| Type of affinity assay | Determination method | LOD | DLR | Ref (s) |
|---|---|---|---|---|
| Glass substrate/Negative photoresist/Polydimethylsiloxane (PDMS) | Microfluidics/Fabrication of microfluidics structure-based polydimethylsiloxane biopolymer on a glass substrate with SU-8 photoresist for label-free detection DNA | 260–280 nm absorbance | 0.000005 A difference at 1.0 V after hybridization | |
| CMOS sensor for imaging | Microfluidic/Presented centrifugation assisted precipitation method for single-step DNA quantification | 10Mng/uL | 10–100Mng/uL | |
| Agarose gel electrophoresis/PCR | Microfluidic/PCR/designed 3D-printed microfluidic chip was and fabricated for droplet-based PCR detection of mRNA | normalized intensity-0.82–0.92 | Jiao et al. (2019) | |
| Ion concentration polarization | Microfluidic Electrokinetic/Sensitive detection of nucleic acids via hybridization on a microarray | 1 nM | 0.1–100 nM | Kim et al. (2020) |
| PDMS | Microfluidic Chip/Designed Power-free Chip in which amplification of Fluorescence signals after hybridization by laminar flow-assisted dendritic | 0.045–0.45 pM | 1 fM–10 pM | Kim et al. (2019) |
| PDMS/Poly (methyl methacrylate) (PMMA) | Flexible microfluidic chip/developed a wearable microfluidic device combined with RPA for simple and rapid amplification of DNA using human body heat | 100 copies/mL in 24 min | 10–105 copies/mL | Kong et al. (2019) |
| PDMS/Polyethylene (PE) | Microfluidic PCR/propose a bubble-free microfluidic PCR device via controlled fluid transfer | 47 copies/single PCR in <3 min | 250–1,000 nM | Lee et al. (2019) |
| Laser diode/PMMA | Microfluidic/Proposed Disposal disc based on a double rotation axes centrifugal microfluidic platform | 100 copies/mL | 0–500 μL | Li et al. (2019a) |
| PDMS | Microfluidic PCR/proposed hierarchical selective electro kinetic concentration PCR as noise resistant quantitative PCR for rapid detection of ultralow-abundance DNA in real biofluids | 0.05 aM | 0–150 mL | Ye and De (2017) |
| Cyclic olefin copolymer (COC) | Microfluidic/based combination of online dynamic magnetic extraction procedure with droplet-based digital PCR | 15.45 ng/mL | 10–100 ng/mL | Perez-Toralla et al. (2019) |
| COC | Microfluidic/RNA was hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags | 757.86 fM | 10-1-10−5 pM | Prada et al. (2019) |
| Polycarbonate/lithium niobate | Microfluidic chip/based on non-equilibrium ionic currents and detects the presence of negative charge on target molecules | 1 pM | 0.001–1 nM | Ramshani et al. (2019) |
| Graphene Oxide (Gox)/Fluorimetric | Fluorimetric-Paired-Emitter-Detector-Diode based on GO high affinity toward single-stranded DNA and its ability to quench the fluorescence | 0.625 uM | 0.625–2.5 uM | Ziółkowski et al. (2020) |
| Au/Fe3O4 NPs/Polydopamine based microbeads | Flow cytometry/Synthesizes Au NPs decorated magnetic MBs exhibit fluorescence signal of each MBs could be collected individually, realizing single MBs-based DNA imaging | 0.1 nM | 0.2–20 nM | Li et al. (2020a) |
Chip-based microfluidic DNA sensor detection techniques.
2.2.2.3 FET-based DNA sensors
FET biosensors are highly sensitive detectors based on 2D materials. They have high carrier mobility owing to their nanoscale dimensions and high volume-to-charge ratio. MXene-based biosensors are a combination of metal carbides and nitrides that have attracted attention because of their unique characteristics (; ). They are used for analyte detection and biosensing applications (Yadav et al., 2021). A combination of MXene-graphene-based FET has been used to detect influenza and 2019-Ncov (Li et al., 2021). A multiscale computer simulation method and experimental approach were employed to investigate the characteristics of flat and crumpled graphene-based biosensors by Hwang et al. (2020) ,who found that the detection limits of buffer and human samples are 600 aM and 20 aM, respectively. Furthermore, the atomic-scale simulation results revealed that the deformation mechanism resulted in electrical hotspots in the channel. This technique can be used to develop reliable fast-track biosensors for medical applications. Figure 11 displays a cross-sectional representation of flat and crumpled graphene FET sensors over a Debye length, represented by the blue curve (Figure 11A), whereas Figure 11B shows the fabrication route. Debye screening is weaker in crumpled graphene, which makes it more sensitive for detecting DNA (Hwang et al., 2020). The insets show the distribution of energy over the K-space.
FIGURE 11
A computational study on single-layer MXene that has potential applications as a DNA detection material with high sensitivity and effectiveness was conducted (Yadav et al., 2021). Computer simulation methods have frequently been used to understand the mechanisms and interactions of materials at the atomic scale (Mustafa et al., 2017; ; ; ; ; ; ; Mustafa Azeem et al., 2018; Mustafa Azeem et al., 2019). An overview of the various FET-based DNA detection techniques reported earlier is listed in Table 7. It is important to point here that LD material has displayed clear advantage on other materials used for designing sensors for health application because of their tunable band structure and ultrathin nature. They also display an improved detection sensitivity. LD materials have been emerged as promising candidate for health industry. Figure 12 summarize LD materials and their application in sensing applications. The division is based on the synthesis, preparation methods, electrochemical and optical properties. The fabrication process of these materials involves process of exfoliation that depends on the chemical environment. Moreover, intercalation constitute chemical vapor deposition (CVD) and electrochemical exfoliation. The material based on LD sensors are used in theranostics image guide application and diagnostics ().
TABLE 7
| Type of affinity assay | Determination method | LOD | DLR | Ref |
|---|---|---|---|---|
| Silicon nitride on SiO2 Substrate | Ion-Sensitive FET/CMOS chemical sensing array operating in current mode for real-time ion imaging and detection of DNA amplification by Isothermal Loop-Mediated | 1.03 μA/pH | Miscourides et al. (2018) | |
| Graphene layer on SiO2/Si Substrate | FET/based on two-dimensional channel of a single graphene layer that can discriminate a single nucleotide polymorphism | 25 aM | 1 Am–100 fM | |
| ISFET chemical frame/Bio Chip | Ion-Sensitive FET/DNA amplification generates protons H+ proportional to the number of DNA copies by isothermal amplification | 16.7 mpH | 1–12 pH | |
| liquid exfoliated graphene | FET/Immunorecognition based on the glutaraldehyde modified liquid exfoliated graphene FET measured by current between the electrodes of drain and source | Sensitivity <3 | 1–106 pM | Zhang et al. (2019b) |
| 3D Graphene | FET/by introducing target miRNA affects the electrical potential of 3D-G and detection of miRNA | 100 pM | 100 pM–100 nM | Song et al. (2020b) |
| Graphene on SiO2/Si Substrate | FET/Trend of Dirac point shift by adsorption of single-stranded DNA studied | Dirac Voltage Shift 10 V | Yi et al. (2019) | |
| Graphene on SiO2 Substrate | FET/Back-gated G-FET-based on engineered hairpin probe DNA with improved sensitivity up to fM level | <10 fM | 1,000 nM–10 fM | |
| Graphene/Magnetic bead | G-FET/that uses clustered regularly interspaced short palindromic repeats technology to enable the digital detection of a target sequence | 1.7 fM | ||
| Metal/SiO2/Ion-sensitive layer | MOSFET/detected DNA by changing dielectric constant and then obtaining its threshold voltage | 0.65 V | ||
| AlGaN/GaN on Si Substrate | Transistor/proposed an electrical double layer gated high electron mobility transistor as DNA sensor | 1 fM | 1 pM–10 fM | |
| Graphene/DNA tweezers probe | FET/Single nucleotide polymorphism sensitivity achieved by observing changes in Dirac point shift and resistance change | 100 pM | 10 uM to 100 nM | Hwang et al. (2018) |
| Single-walled carbon nanotubes (CNT) on SiO2 | FET/Developed floating electrode-based DNA sensor with controllable responses based on Langmuir theory | 100 fM | 1 nM–10 uM | Kim et al. (2012) |
| MoS2/Go/FET on Cu Substrate | FET/DNA detection by charge transfer through MoS2 between graphene and DNA | 10 aM | 10 aM–100 p.m | |
| AuNPs/single-walled carbon nanotube (SWCNT) on SiO2 | FET/DNA detection depend on the percolation paths of SWNTs in conduction channels | 100 fM | 0–1 nM | |
| CNT and graphene/PCB/PAN Probe | FET/reported array of Ion-Sensitive Field-Effect Transistors for detection of nucleic acid molecules | 1 nM | 1 Nm–1 μM | |
| Graphene layer with Pyrenebutanoic acid succinimidyl ester | FET/The negatively charged effect of DNA molecule used for FET/graphene-based DNA sensor. Non-covalent bonded DNA caused a “left” shift of the Dirac point | 3 nM | 1–32 nM | |
| Polycrystalline Si Nanowire | FET/enhanced FET sensitivity through using chimeric DNAs with methylated neutral nucleotides as probes | 0.1 fM | 0.1–10 fM | Hu et al. (2018) |
| Crumpled/flat graphene | FET Computational simulations reveal that deformed graphene could exhibit a change in band-gap, allowing an exponential change in the source-drain current from small numbers of charges | (600 zM) [crumpled 2 pM(flat)] | 10−3 M–10−20 M | Hwang et al. (2020) |
| CNT devices embedded in polymer substrates substrate | FET/CNT based flexible circuits for DNA sensors using Raman spectroscopy | 160 nM | Kang et al. (2008) | |
| Liquid coplaner Graphene FET | FET/Designed Liquid coplanar-gate graphene FET to detect and distinguish between single-stranded and double-stranded DNA molecules | 1 nM | 0–10 nM | Kim et al. (2018) |
| Graphene, PS Brush, SiO2, Si | FET/Used interfacial polymer brush layer, which is inserted between graphene and SiO2 to enhance the electrical properties of the sensor | 12-mer ssDNA10 | pM/μL | Ku et al. (2018) |
| Au electrodes/polymer substrate/PDMS/PMMA | MOSFET/developed a miRNA sensor using an electrical double layer gated FET biosensor with enhanced sensitivity and stability | 100 fM | 100–1,000 fM | Kuo et al. (2019) |
| CNT/aryldiazonium salts/Si Substrate | Designed single-point-functionalized CNTFETs have been used to sense conformational changes and binding events in nucleic acid structures from intrinsic molecular charge (Sp3 defects) | 20-mer target DNA | 100 nM | Lee et al. (2018) |
| Graphene/Au Gate/Glass substrate/Electrolyte | FET/The mechanism of this novel DNA sensor is that the gate potential drop is induced by DNA immobilization and hybridization on the Au gate electrode | 1 fM | 1 fM–5 μM | Li et al. (2019b) |
| Graphene/MoS2 heterostructures/on a sapphire substrate/Laser | Photoluminescence PL/characteristics of the grown graphene/MoS2 film are used for label-free and selective detection of DNA hybridization | 1 aM | 0.1∼1 fM | Loan et al. (2014) |
| MoS2/Si/SiO2 substrate/Ti and Au Electrode | FET/Developed label-free and direct hybridization assay using MoS2-FET biosensor for ultrasensitive detection of miRNA | 0.03 fM | 0.1 fM–10 nM | Majd et al. (2018) |
| MoS2/Phosphorodiamidate morpholino oligos (PMO) | FET/Developed PMO-modified MoS2 FET biosensor for detecting DNA based on PMO-DNA hybridization with high sensitivity and specificity | 6 fM | 10 fM–1 nM | Mei et al. (2018) |
| Graphene embedded nanochannel device/Theoretical DFT-NEGFT | Developed Graphene embedded nanochannel device that effectively controls the motion of nucleobases via p–p interaction and deciphers the ultrasensitivity of individual bases, one by one, in real time | Fano resonance-driven conductance of individual bases | Min et al. (2011) | |
| Si3N4/Al on Si/SiO2Ag/AgCl References electrode | CMOS FET/based on ion-sensitive field-effect transistor array using in-pixel quantization and compensation of sensor non-idealities | 3.2 μs/pH | 12.8 ns–33.1 ns | Moser et al. (2018) |
| Al2O3 film/aluminum Floating Gate electrodes/polyethylene terephthalate substrates | FET/presented electronic transduction of DNA hybridization by coupling OCMFETs and hairpin shaped probes | 100pM | 10 nM–10 pM | Napoli et al. (2018) |
| Si3N4/doped Si substrate/Si nanonets (SiNN)/nanowires | Si nanonet FET/reported field-effect silicon nanonet transistors for DNA sensing | 30/30 tested devices | Nguyen et al. (2019) | |
| Graphene/SiO2/Si substrate/Au/Cr electrode | FET/developed a COVID-19 FET sensor in which the SARS-CoV-2 spike antibody is conjugated to a graphene sheet, which is used as the sensing area | 2.42 × 102 copies/mL | 50–100 copies | Seo et al. (2020) |
| Fluorescence | FET/presented DNA sensor based on graphene and magnetic nanoparticles | 1 pM | 1 pM–10 nM | Sun et al. (2019a) |
| Carbon nanotube/Pd electrodes/SiO2 substrate | FET/a suspended CNT based FET was fabricated by utilizing the surface tension of liquid silver to suspend a CNT between two Pd electrodes for the detection of DNA hybridization | 10 aM | 1 pM–10 aM | Sun et al. (2019c) |
| Monolayer graphene/Ag/AgCl electrode/ITO glass substrate | G-FET/Using the graphene as the electric channel, fabricated G-FET sensor that can be used for detection of RNA | 0.1 fM | 0.1 fM to 1 pM | Tian, (2018) |
| Liquid exfoliated graphene/solution-gated FET/silver paste | FET/fabricated bioLEG-SgFETs and demonstrate their bio-application in single-strand DNA detection | 10 fM | 10-3-105 pM | Wang and Jia (2018) |
| Silicon wafer FET | FET/fabricated side-gated silicon nanowire FET was using complementary metal oxide semiconductor technology | 0.83 fM | 10 fM–10 nM | Wu et al. (2018) |
FET-based DNA detectors.
FIGURE 12
3 Challenges and commercial applications
Sensors play an important role in our daily life specifically in the healthcare sector as DNA detection and its diagnostic involve curing diseases. The current trend and developing sensors are now hot topics. They have increasing demand after the nCov-19. A big challenge involving the development of DNA detection sensors requires components with on-site and rapid detection and detection limit. Before commercialization, it is very important to handle the stability and reproducibility of the samples. Another major challenge concerns the storage as handling the short lifetime of the samples and re-usage. The conventional detection methods are based on PCR techniques which are very expensive and time-consuming. It is one of the big challenges that involve the detection of ultra-low concentrations of any analyte. Another challenge involves the response time for detection. Developing accurate and economical DNA sensors is a present concern. Presently, DNA detection with nanopore technology has resolved this issue and these materials have already displayed unique characteristics. Compared to conventional methods 2D materials-based FET sensors involve the electric field to create a charge to interact with DNA and change in current results in the form of a signal to detect.
4 Conclusion
Infectious diseases have posed a challenge in the past few years. Biosensing and biotechnology are emerging fields. In particular, DNA biosensors have potential research applications because of their chemical properties as well as reliable and fast detection.
We have reviewed different types of biosensors and listed the fabrication techniques and materials used to develop them. Conventional methods for developing in vitro diagnostics are time-consuming and require multiple trials and centralized technologies. There are diverse techniques and strategies for developing sensors, including, but not limited to, collecting samples, and implementing integrated diagnostics for biological applications. LD materials are considered as alternate to traditional materials due to their nanosized thickness and compatible nature. Currently, understanding nature of these materials in biological environment is major challenge. Critical challenges exist in transforming the optimal clinical treatment of infectious diseases from trial to translational research.
Statements
Author contributions
MAz prepared the initial draft and collected the literature review, and MS collected most of the information. MZ, MAa, BS, and MWA reviewed and proofread the manuscript. All the authors have approved this manuscript.
Funding
This work is supported by the Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Saudi Arabia, under the Reviewing Researcher Track (Grant No. 2389).
Acknowledgments
Authors would like to thank King Faisal University for supporting this research.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
1
AbdulwahabM. H. M.MoserN.Rodriguez-ManzanoJ.GeorgiouP. (2018). A CMOS bio-chip combining pH sensing, temperature regulation and electric field generation for DNA detection and manipulation. IEEE Int. Symp. Circuits Syst., 1–5.
2
AminiB.KamaliM.SaloutiM.YaghmaeiP. (2018). Spectrophotometric, colorimetric and visually detection of Pseudomonas aeruginosa ETA gene based gold nanoparticles DNA probe and endonuclease enzyme. Spectrochim. Acta - Part A Mol. Biomol. Spectrosc.199, 421–429. 10.1016/j.saa.2018.03.056
3
AminiH.BanŽ.FergerM.LorenzenS.RauchF.FriedrichA.et al (2020). Tetracationic bis-triarylborane 1,3-butadiyne as a combined fluorimetric and Raman probe for simultaneous and selective sensing of various DNA, RNA, and proteins. Chemistry26, 6017–6028. 10.1002/chem.201905328
4
AyoibA.HashimU.GopinathS. C. B.ThivinaV.ArshadM. K. M. (2020). Design and fabrication of PDMS microfluidics device for rapid and label-free DNA detection. Appl. Phys. A Mat. Sci. Process.126, 193–198. 10.1007/s00339-020-3337-7
5
AzabM. Y.HameedM. F. O.NasrA. M.ObayyaS. S. A. (2018). Label free detection for DNA hybridization using surface plasmon photonic crystal fiber biosensor. Opt. Quantum Electron.50, 68–13. 10.1007/s11082-017-1302-2
6
AzeemM. M.LiZ.WangQ.AmjadQ. M. N.ZubairM.AhmedO. M. H. (2018a). “Classical molecular dynamics study for defect sink behavior in oxide dispersed strengthened alloys,” in Proceedings of 2018 15th International Bhurban Conference on Applied Sciences and Technology (Islamabad, Pakistan: IBCAST), 12–15. 10.1109/IBCAST.2018.8312177
7
AzeemM. M.LiZ.WangQ.HussianA. (2018b). Molecular dynamics simulation study on the possible factors affecting stability of ODS steel. IOP Conf. Ser. Mater. Sci. Eng.389, 012003. 10.1088/1757-899X/389/1/012003
8
AzeemM. M.WangQ.LiZ.ZhangY. (2020). Dislocation-oxide interaction in Y2O3 embedded Fe: A molecular dynamics simulation study. Nucl. Eng. Technol.52, 337–343. 10.1016/j.net.2019.07.011
9
AzeemM. M.WangQ.ZhangY.LiuS.ZubairM. (2019a). Effect of grain boundary on diffusion of P in alpha-Fe: A molecular dynamics study. Front. Phys.7, 1–7. 10.3389/fphy.2019.00097
10
AzeemM. M.WangQ.ZubairM. (2019b). Atomistic simulations of nanoindentation response of irradiation defects in iron. Sains Malays.48, 2029–2039. 10.17576/jsm-2019-4809-24
11
AzeemM. M.YunD.ZubairM. (2021). Atomic insights on interaction mechanism of dislocation with void/impurity/precipitates in bcc iron. Int. Conf. Nucl. Eng. Proc. ICONE2, 1–7. 10.1115/ICONE28-65197
12
BabarZ. U. D.Della VenturaB.VelottaR.IannottiV. (2022). Advances and emerging challenges in MXenes and their nanocomposites for biosensing applications. RSC Adv.12, 19590–19610. 10.1039/d2ra02985e
13
BakshiS. F.GuzN.ZakharchenkoA.DengH.TumanovA. V.WoodworthC. D.et al (2017). Magnetic field-activated sensing of mRNA in living cells. J. Am. Chem. Soc.139, 12117–12120. 10.1021/jacs.7b06022
14
BakshiS.ZakharchenkoA.MinkoS.KolpashchikovD. M.KatzE. (2019). Towards nanomaterials for cancer theranostics: A system of dna-modified magnetic nanoparticles for detection and suppression of rna marker in cancer cells. Magnetochemistry5, 24. 10.3390/magnetochemistry5020024
15
BalajiA.YangS.WangJ.ZhangJ. (2019). Graphene oxide-based nanostructured DNA sensor. Biosensors9, 74. 10.3390/bios9020074
16
BanerjeeI.AralaguppeS. G.LapinsN.ZhangW.KazemzadehA.SönnerborgA.et al (2019). Microfluidic centrifugation assisted precipitation based DNA quantification. Lab. Chip19, 1657–1664. 10.1039/c9lc00196d
17
BechnakL.El KurdiR.PatraD. (2020). Fluorescence sensing of nucleic acid by curcumin encapsulated poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) based nanocapsules. J. Fluoresc.30, 547–556. 10.1007/s10895-020-02528-9
18
BenelmekkiM.KimJ.-H. (2023). Stimulus-responsive ultrathin films for bioapplications: A concise review. Molecules28, 1020. 10.3390/molecules28031020
19
BiQ.GanX.YuanR.XiangY. (2020). Copper-free click chemistry-mediated cyclic ligation amplification for highly sensitive and non-label electrochemical detection of gene mutation. J. Electrochem. Soc.167, 027535. 10.1149/1945-7111/ab6a81
20
BolotskyA.ButlerD.DongC.GeraceK.GlavinN. R.MuratoreC.et al (2019). Two-dimensional materials in biosensing and healthcare: From in vitro diagnostics to optogenetics and beyond. ACS Nano13, 9781–9810. 10.1021/acsnano.9b03632
21
BruijnsB.van AstenA.TiggelaarR.GardeniersH. (2016). Microfluidic devices for forensic DNA analysis: A review. Biosensors6, 41–35. 10.3390/bios6030041
22
CamposR.BormeJ.GuerreiroJ. R.MachadoG. J.CerqueiraM. F.PetrovykhD. Y.et al (2019). Attomolar label-free detection of DNA hybridization with electrolyte-gated graphene field-effect transistors. ACS Sensors4, 286–293. 10.1021/acssensors.8b00344
23
CantorD. (1998). Crafting science: A sociohistory of the quest for the genetics of cancer. Bull. Hist. Med.72, 590–592. 10.1353/bhm.1998.0127
24
CaoH.ZhouX.ZengY. (2019). Microfluidic exponential rolling circle amplification for sensitive microRNA detection directly from biological samples. Sens. Actuators. B. Chem.279, 447–457. 10.1016/j.snb.2018.09.121
25
CaoX.ZhangK.YanW.XiaZ.HeS.XuX.et al (2020). Calcium ion assisted fluorescence determination of microRNA-167 using carbon dots-labeled probe DNA and polydopamine-coated Fe(3)O(4) nanoparticles. Mikrochim. Acta187, 212. 10.1007/s00604-020-4209-8
26
ChenS.SunY.XiaY.LvK.ManB.YangC. (2020). Donor effect dominated molybdenum disulfide/graphene nanostructure-based field-effect transistor for ultrasensitive DNA detection. Biosens. Bioelectron.156, 1–7. 10.1016/j.bios.2020.112128
27
ChenY.TaiT.HsuC.SarangadharanI.KumarA.WangH.et al (2018). Sensors and Actuators B: Chemical Direct detection of DNA using electrical double layer gated high electron mobility transistor in high ionic strength solution with high sensitivity and speci fi city. Sensors Actuators B. Chem.271, 110–117. 10.1016/j.snb.2018.05.119
28
ChenZ.PengY.XieX.FengY.LiT.LiS.et al (2019). Dendrimer-functionalized superparamagnetic nanobeacons for real-time detection and depletion of HSP90α mRNA and MR imaging. Theranostics9, 5784–5796. 10.7150/thno.36545
29
CheungL. S.WeiX.MartinsD.SongY.-A. (2018). Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip. Biomicrofluidics12, 014104. 10.1063/1.5009719
30
ChiticaruE. A.PilanL.DamianC. M.VasileE.BurnsJ. S.IonitaM. (2019). Influence of graphene oxide concentration when fabricating an electrochemical biosensor for DNA detection. Biosensors9, 113–118. 10.3390/bios9040113
31
DasC. M.GuoY.KangL.PoenarD. P.XiongJ.RamaswamyY.et al (2021). Improving the sensitivity of SPR sensors with Au–Ag alloys and 2D materials — A simulation-based approach. Adv. Theory Simulations4, 1–10. 10.1002/adts.202100292
32
DengH.ChaiY.YuanR.YuanY. (2020). In situ formation of multifunctional DNA nanospheres for a sensitive and accurate dual-mode biosensor for photoelectrochemical and electrochemical assay. Anal. Chem.92, 8364–8370. 10.1021/acs.analchem.0c00918
33
DengS.WangP.YuX. (2017). Phase-sensitive surface plasmon resonance sensors: Recent progress and future prospects. Sensors Switz.17, 2819. 10.3390/s17122819
34
DinterF.BurdukiewiczM.SchierackP.LehmannW.NestlerJ.DameG.et al (2019). Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip. Anal. Bioanal. Chem.411, 7725–7735. 10.1007/s00216-019-02199-x
35
DongX.LauC. M.LohaniA.MhaisalkarS. G.KasimJ.ShenZ.et al (2008). Electrical detection of femtomolar DNA via gold-nanoparticle enhancement in carbon-nanotube-network field-effect transistors. Adv. Mat.20, 2389–2393. 10.1002/adma.200702798
36
FengE.ZhengT.TianY. (2019). Dual-mode Au nanoprobe based on surface enhancement Raman scattering and colorimetry for sensitive determination of telomerase activity both in cell extracts and in the urine of patients. ACS Sensors4, 211–217. 10.1021/acssensors.8b01244
37
FukuX.BakerP.IwuohaE. (2020). Influence of quantum dot surface on electrochemical DNA sensing mechanism. ChemElectroChem7, 770–781. 10.1002/celc.201902079
38
GanguliA.WatanabeY.HwangM. T.HuangJ. C.BashirR. (2018). Robust label-free microRNA detection using one million ISFET array. Biomed. Microdevices20, 45. 10.1007/s10544-018-0290-8
39
GaoJ.ZhangH.WangZ. (2020a). A DNA tetrahedron nanoprobe-based fluorescence resonance energy transfer sensing platform for intracellular tumor-related miRNA detection. Analyst145, 3535–3542. 10.1039/C9AN02610J
40
GaoY.QiangL.ChuY.HanY.ZhangY.HanL. (2020b). Microfluidic chip for multiple detection of miRNA biomarkers in breast cancer based on three-segment hybridization. AIP Adv.10, 045022. 10.1063/1.5137784
41
GaoZ.XiaH.ZaubermanJ.TomaiuoloM.PingJ.ZhangQ.et al (2018). Detection of sub-fM DNA with target recycling and self-assembly ampli fi cation on graphene field-E ff ect biosensors, Nano Lett18. 3509-3515. 10.1021/acs.nanolett.8b00572
42
García-MendiolaT.Gutiérrez-SánchezC.GibajaC.TorresI.Busó-RogeroC.ParienteF.et al (2020). Functionalization of a few-layer antimonene with oligonucleotides for DNA sensing. ACS Appl. Nano Mat.3, 3625–3633. 10.1021/acsanm.0c00335
43
GoelA.RewariS.VermaS.GuptaR. S. (2018). Dielectric modulated triple metal gate all around MOSFET (TMGAA) for DNA bio- molecule detection. 1, 24–25.
44
GuerriniL.Morla-FolchJ.Gisbert-QuilisP.XieH.Alvarez-PueblaR. A. (2016). Label-free direct surface-enhanced Raman scattering (SERS) of nucleic acids (Conference Presentation). SPIE Proceeding9722, 23. 10.1117/12.2217653
45
GuoJ.ChenY.JiangY.JuH. (2017). Polyadenine-modulated DNA conformation monitored by surface-enhanced Raman scattering (SERS) on multibranched gold nanoparticles and its sensing application. Chemistry23, 9332–9337. 10.1002/chem.201700883
46
GuoQ.YuY.ZhangH.CaiC.ShenQ. (2020). Electrochemical sensing of exosomal MicroRNA based on hybridization chain reaction signal amplification with reduced false-positive signals. Anal. Chem.92, 5302–5310. 10.1021/acs.analchem.9b05849
47
GuoS. R.LinJ.PenchevM.YengelE.GhazinejadM.OzkanC. S.et al (2011). Label free DNA detection using large area graphene based field effect transistor biosensors. J. Nanosci. Nanotechnol.11, 5258–5263. 10.1166/jnn.2011.3885
48
HajianR.BalderstonS.TranT.deBoerT.EtienneJ.SandhuM.et al (2019). Detection of unamplified target genes via CRISPR-Cas9 immobilized on a graphene field-effect transistor. Nat. Biomed. Eng.3, 427–437. 10.1038/s41551-019-0371-x
49
HameedM. F. O.SaadeldinA. S.ElkaramanyE. M. A.ObayyaS. S. A. (2017). Label-free highly sensitive hybrid plasmonic biosensor for the detection of DNA hybridization. J. Light. Technol.35, 4851–4858. 10.1109/jlt.2017.2733720
50
HanB.ZhangY.ZhuL.ChenX.-H.MaZ.ZhangX.-L.et al (2018a). Direct laser scribing of AgNPs@RGO biochip as a reusable SERS sensor for DNA detection. Sensors Actuators B Chem10.1063/1.5137784
51
HanS.LiuW.ZhengM.WangR. (2020). Label-free and ultrasensitive electrochemical DNA biosensor based on urchinlike carbon nanotube-gold nanoparticle nanoclusters. Anal. Chem.92, 4780–4787. 10.1021/acs.analchem.9b03520
52
HanS.LockeA. K.OaksL. A.ChengY.-S. L.CotéG. L. (2018b). Development of a free-solution SERS-based assay for point-of-care oral cancer biomarker detection using DNA-conjugated gold nanoparticles. Proc. SPIE, 1050104. 10.1117/12.2290516
53
HanS.LockeA. K.OaksL. A.ChengY. L.CotéG. L.HanS.et al (2019). Nanoparticle-based assay for detection of S100P mRNA using surface-enhanced Raman spectroscopy. J. Biomed. Opt.24, 1–9. 10.1117/1.jbo.24.5.055001
54
HaoK.HeY.LuH.PuS.ZhangY.DongH.et al (2017). High-sensitive surface plasmon resonance microRNA biosensor based on streptavidin functionalized gold nanorods-assisted signal amplification. Anal. Chim. Acta954, 114–120. 10.1016/j.aca.2016.12.006
55
HeY.YangX.YuanR.ChaiY. (2019). A novel ratiometric SERS biosensor with one Raman probe for ultrasensitive microRNA detection based on DNA hydrogel amplification. J. Mat. Chem. B7, 2643–2647. 10.1039/c8tb02894j
56
HlongwaneG. N.Dodoo-ArhinD.WamwangiD.DaramolaM. O.MoothiK.IyukeS. E. (2019). DNA hybridisation sensors for product authentication and tracing: State of the art and challenges. South Afr. J. Chem. Eng.27, 16–34. 10.1016/j.sajce.2018.11.002
57
HuW. P.TsaiC. C.YangY. S.ChanH. W. H.ChenW. Y. (2018). Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe. Sci. Rep.8, 12598–8. 10.1038/s41598-018-30996-4
58
HuangY.TaoM.LuoS.ZhangY.SituB.YeX.et al (2020). A novel nest hybridization chain reaction based electrochemical assay for sensitive detection of circulating tumor DNA. Anal. Chim. Acta1107, 40–47. 10.1016/j.aca.2020.02.006
59
HügleM.BehrmannO.RaumM.HufertF. T.UrbanG. A.DameG. (2020). A lab-on-a-chip for free-flow electrophoretic preconcentration of viruses and gel electrophoretic DNA extraction. Analyst145, 2554–2561. 10.1039/C9AN02333J
60
HwangM. T.HeiranianM.KimY.YouS.LeemJ.TaqieddinA.et al (2020). Ultrasensitive detection of nucleic acids using deformed graphene channel field effect biosensors. Nat. Commun.11, 1543. 10.1038/s41467-020-15330-9
61
HwangM. T.WangZ.PingJ.BanD. K.ShiahZ. C.AntonschmidtL.et al (2018). DNA nanotweezers and graphene transistor enable label-free genotyping. Adv. Mat.30, e1802440. 10.1002/adma.201802440
62
JayadevimanoranjithamJ.NarayananS. S. (2020). Fabrication of poly o-cresophthalein complexone film modified electrode and its application to simultaneous determination of purine bases in denatured DNA. Mat. Sci. Eng. C. Mat. Biol. Appl.108, 110353. 10.1016/j.msec.2019.110353
63
JiaL.-P.FengZ.ZhaoR.-N.MaR.-N.ZhangW.ShangL.et al (2020). Enzyme-free and triple-amplified electrochemical sensing of 8-hydroxy-2′-deoxyguanosine by three kinds of short pDNA-driven catalyzed hairpin assemblies followed by a hybridization chain reaction. Analyst145, 3605–3611. 10.1039/D0AN00233J
64
JiangJ.WuH.SuY.LiangY.ShuB.ZhangC. (2020a). Electrochemical cloth-based DNA sensors (ECDSs): A new class of electrochemical gene sensors. Anal. Chem.92, 7708–7716. 10.1021/acs.analchem.0c00669
65
JiangP.TanM.ZhaoL.WangY.PanT.ChombaH.et al (2020b). A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification. Mat. Express10, 206–213. 10.1166/mex.2020.1633
66
JiangW.YinH.ZhouY.DuanJ.LiH.WangM.et al (2018). A novel electrochemiluminescence biosensor for the detection of 5-methylcytosine, TET 1 protein and β-glucosyltransferase activities based on gold nanoclusters-H2O2 system. Sensors Actuators B Chem.274, 144–151. 10.1016/j.snb.2018.07.149
67
JiangZ. Y.JiangX. X.SuS.WeiX. P.LeeS. T.HeY. (2012). Silicon-based reproducible and active surface-enhanced Raman scattering substrates for sensitive, specific, and multiplex DNA detection. Appl. Phys. Lett.100, 203104. 10.1063/1.3701731
68
JiaoZ.ZhaoL.TangC.ShiH.WangF.HuB. (2019). Droplet-based PCR in a 3D-printed microfluidic chip for miRNA-21 detection. Anal. Methods11, 3286–3293. 10.1039/c9ay01108k
69
KangJ.LeeJ.KimT. H.ParkJ.SeongM. J.HongS. (2008). Large-scale assembly of carbon nanotube-based flexible circuits for DNA sensors. Nanotechnology19, 135305. 10.1088/0957-4484/19/13/135305
70
KannegullaA.LiuY.WuB.ChengL.-J. (2018a10728). Enhanced molecular beacon based DNA detection using plasmonic open-ring nanoarrays. Proc. SPIE. 10.1117/12.2321234
71
KannegullaA.LiuY.WuB.ChengL. J. (2018b). Plasmonic open-ring nanoarrays for broadband fluorescence enhancement and ultrasensitive DNA detection. J. Phys. Chem. C122, 770–776. 10.1021/acs.jpcc.7b09769
72
KhalilI.YehyeW. A.JulkapliN. M.RahmatiS.SinaA. A. I.BasirunW. J.et al (2019). Graphene oxide and gold nanoparticle based dual platform with short DNA probe for the PCR free DNA biosensing using surface-enhanced Raman scattering. Biosens. Bioelectron.131, 214–223. 10.1016/j.bios.2019.02.028
73
KhoderR.Korri-YoussoufiH. (2020). E-DNA biosensors of M. tuberculosis based on nanostructured polypyrrole. Mat. Sci. Eng. C. Mat. Biol. Appl.108, 110371. 10.1016/j.msec.2019.110371
74
KimB.LeeJ.NamgungS.KimJ.ParkJ. Y.LeeM. S.et al (2012). DNA sensors based on CNT-FET with floating electrodes. Sensors Actuators, B Chem.169, 182–187. 10.1016/j.snb.2012.04.063
75
KimH.HyejeongJ.MoonJ.ByunJ.JeongJ.JuyeonJ.et al (2019). Metal–organic framework coating for the preservation of silver nanowire surface‐enhanced Raman scattering platform. Adv. Mat. Interfaces6, 1900427. 10.1002/admi.201900427
76
KimJ.-H.BenelmekkiM. (2018). “Bottom-up synthesis of hybrid carbon nanoscrolls,” in Emerging applications of nanoparticles and architecture nanostructures. Editors Barhoum,A.MakhloufA. N.(Elsevier). 10.1016/B978-0-323-51254-1.00018-X
77
KimJ.-H.BenelmekkiM. (2019). “Two-dimensional nanostructures for biomedical applications,” in Nanostructured thin film: Fundamentals and applications. Editors Benelmekki,M.ErbeA. B. T.-F. of N. (Elsevier). 10.1016/B978-0-08-102572-7.00004-0
78
KimJ. H.BohraM.SinghV.CassidyC.SowwanM. (2014). Smart composite nanosheets with adaptive optical properties. ACS Appl. Mat. Interfaces6, 13339–13343. 10.1021/am5041708
79
KimJ.SahloulS.OrozalievA.DoV. Q.PhamV. S.MartinsD.et al (2020). Microfluidic electrokinetic preconcentration chips: Enhancing the detection of nucleic acids and exosomes. IEEE Nanotechnol. Mag.14, 18–34. 10.1109/MNANO.2020.2966064
80
KimJ. W.JanghaKuY. G. M.KimS.LeeE.ChoK.et al (2018). Liquid coplanar-gate organic/graphene hybrid electronics for label-free detection of single and double-stranded DNA molecules. Org. Electron.62, 163–167. 10.1016/j.orgel.2018.07.032
81
KimY.-J.HosokawaK.MaedaM. (2019). Sensitivity enhancement of MicroRNA detection using a power-free microfluidic chip. Anal. Sci. Int. J. Jpn. Soc. Anal. Chem.35, 1227–1236. 10.2116/analsci.19P211
82
KongM.LiZ.WuJ.HuJ.ShengY.WuD.et al (2019). Talanta A wearable microfluidic device for rapid detection of HIV-1 DNA using recombinase polymerase amplification. Talanta205, 1–8. 10.1016/j.talanta.2019.120155
83
KowalczykA.KrajczewskiJ.KowalikA.WeyherJ. L.DzięcielewskiI.ChłopekM.et al (2019). New strategy for the gene mutation identification using surface enhanced Raman spectroscopy (SERS). Biosens. Bioelectron.132, 326–332. 10.1016/j.bios.2019.03.019
84
KrishnanS.SyedZ. Q. (2022). Colorimetric visual sensors for point-of-needs testing. Sensors Actuators Rep.4, 100078. 10.1016/j.snr.2022.100078
85
KuG. M.KimJ. W.JangY. h.KimS.LimK.LeeW. H. (2018). Interfacial polymer brush layer for DNA sensors based on graphene transistors. Fibers Polym.19, 2483–2488. 10.1007/s12221-018-8608-y
86
KuoW. C.SarangadharanI.PulikkathodiA. K.ChenP. H.WangS. L.WuC. R.et al (2019). Investigation of electrical stability and sensitivity of electric double layer gated field-effect transistors (FETs) for miRNA detection. Sensors Switz.19, 1484. 10.3390/s19071484
87
LapitanL. D. S.XuY.GuoY.ZhouD. (2019). Combining magnetic nanoparticle capture and poly-enzyme nanobead amplification for ultrasensitive detection and discrimination of DNA single nucleotide polymorphisms. Nanoscale11, 1195–1204. 10.1039/c8nr07641c
88
LednickýT.BonyárA. (2020). Large scale fabrication of ordered gold nanoparticle-epoxy surface nanocomposites and their application as label-free plasmonic DNA biosensors. ACS Appl. Mat. Interfaces12, 4804–4814. 10.1021/acsami.9b20907
89
LeeS. H.SongJ.ChoB.HongS. G.HoxhaO.KangT.et al (2019). Bubble-free rapid microfluidic PCR. Biosens. Bioelectron.126, 725–733. 10.1016/j.bios.2018.10.005
90
LeeY.TrocchiaS. M.WarrenS. B.YoungE. F.VernickS.ShepardK. L. (2018). Electrically controllable single-point covalent functionalization of spin-cast carbon- nanotube field-effect transistor arrays. ACS Nano12, 9922–9930. 10.1021/acsnano.8b03073
91
LeiP.ZhouY.ZhuR.LiuY.DongC.ShuangS. (2020). Novel strategy of electrochemical analysis of DNA bases with enhanced performance based on copper-nickel nanosphere decorated N,B-doped reduced graphene oxide. Biosens. Bioelectron.147, 111735. 10.1016/j.bios.2019.111735
92
LiC.LiG.LiuH.XiaoZ.JinM.YuanC. (2020a). A novel gold nanoparticles decorated magnetic microbead-based molecular beacon for DNA multiplexing detection by flow cytometry. Anal. Chim. Acta1110, 19–25. 10.1016/j.aca.2020.02.063
93
LiF.LiF.MaoX.LiF.LiM.ShenJ.et al (2020b). Ultrafast DNA sensors with DNA framework-bridged hybridization reactions. J. Am. Chem. Soc.142, 9975–9981. 10.1021/jacs.9b13737
94
LiH.RothbergL. (2004). Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proc. Natl. Acad. Sci. U. S. A.101, 14036–14039. 10.1073/pnas.0406115101
95
LiJ.ZhaoL.WenD.LiX.YangH.WangD.et al (2020c). Electrochemical CYFRA21-1 DNA sensor with PCR-like sensitivity based on AgNPs and cascade polymerization. Anal. Bioanal. Chem.412, 4155–4163. 10.1007/s00216-020-02652-2
96
LiL.MiaoB.LiZ.SunZ.PengN. (2019a). Sample-to-Answer hepatitis B virus DNA detection from whole blood on a centrifugal microfluidic platform with double rotation axes. ACS Sensors4, 2738–2745. 10.1021/acssensors.9b01270
97
LiM.LinH.PaidiS. K.MesyngierN.PreheimS.BarmanI. (2020d). A fluorescence and surface-enhanced Raman spectroscopic dual-modal aptasensor for sensitive detection of cyanotoxins. ACS Sensors5, 1419–1426. 10.1021/acssensors.0c00307
98
LiS.HuangK.FanQ.YangS.ShenT.MeiT.et al (2019b). Highly sensitive solution-gated graphene transistors for label-free DNA detection. Biosens. Bioelectron.136, 91–96. 10.1016/j.bios.2019.04.034
99
LiX.-Y.CuiY.-X.DuY.-C.TangA.-N.KongD.-M. (2020e). Isothermal cross-boosting extension-nicking reaction mediated exponential signal amplification for ultrasensitive detection of polynucleotide kinase. Analyst145, 3742–3748. 10.1039/c9an02569c
100
LiY.HollN. J.LiY.PengZ.HollN. J.HassanR.et al (2021). MXene–graphene field-effect transistor sensing of influenza virus and SARS-CoV-2. ACS Omega6, 6643–6653. 10.1021/acsomega.0c05421
101
LiaoH.ZhouY.ChaiY.YuanR. (2018). An ultrasensitive electrochemiluminescence biosensor for detection of MicroRNA by in-situ electrochemically generated copper nanoclusters as luminophore and TiO(2) as coreaction accelerator. Biosens. Bioelectron.114, 10–14. 10.1016/j.bios.2018.05.011
102
LiuD.ZhangX.ZhaoJ.ChenS.YuanR. (2020a). An ultrasensitive sensing platform for microRNA-155 based on H(2)O(2) quenched hydroxide-dependent ECL emission of PFO Pdots. Biosens. Bioelectron.150, 111872. 10.1016/j.bios.2019.111872
103
LiuG.MaX.TangY.MiaoP. (2020b). Ratiometric fluorescence method for ctDNA analysis based on the construction of a DNA four-way junction. Analyst145, 1174–1178. 10.1039/d0an00044b
104
LiuP.YangX.SunS.WangQ.WangK.HuangJ.et al (2013). Enzyme-free colorimetric detection of DNA by using gold nanoparticles and hybridization chain reaction amplification. Anal. Chem.85, 7689–7695. 10.1021/ac4001157
105
LiuQ.SunX.LiuM.JinY.LiB. (2020c). G-triplex molecular beacon‒based fluorescence biosensor for sensitive detection of small molecule-protein interaction via exonuclease III‒assisted recycling amplification. Sensors Actuators B-chemical310, 127804. 10.1016/j.snb.2020.127804
106
LiuS.WeiM.WangX.ShiC.KuangS.MaC. (2020d). An ultrasensitive electrochemical DNA sensing strategy free from pre-immobilization via G-quadruplex based homogenous proximity hybridization. Talanta210, 120628. 10.1016/j.talanta.2019.120628
107
LiuY.ChenX.MaQ. (2018). A novel amplified electrochemiluminescence biosensor based on Au NPs@PDA@CuInZnS QDs nanocomposites for ultrasensitive detection of p53 gene. Biosens. Bioelectron.117, 240–245. 10.1016/j.bios.2018.06.023
108
LiuY.HuN. (2007). Loading/release behavior of (chitosan/DNA)n layer-by-layer films toward negatively charged anthraquinone and its application in electrochemical detection of natural DNA damage. Biosens. Bioelectron.23, 661–667. 10.1016/j.bios.2007.07.009
109
LiuY.NieY.WangM.ZhangQ.MaQ. (2020e). Distance-dependent plasmon-enhanced electrochemiluminescence biosensor based on MoS(2) nanosheets. Biosens. Bioelectron.148, 111823. 10.1016/j.bios.2019.111823
110
LiuY.TuY.WuH.ZhangH.ChenH.ZhouG.et al (2020f). A renewable DNA biosensor for sensitive detection of DNA methyltransferase activity based on cascade signal amplification. Sensors Actuators B Chem.313, 128029. 10.1016/j.snb.2020.128029
111
LiuZ.LeiS.ZouL.LiG.XuL.YeB. (2020). Highly ordered 3D electrochemical DNA biosensor based on dual orientation controlled rolling motor and graftable tetrahedron DNA. Biosens. Bioelectron.147, 111759. 10.1016/j.bios.2019.111759
112
LoanP. T. K.ZhangW.LinC. T.WeiK. H.LiL. J.ChenC. H. (2014). Graphene/MoS2 heterostructures for ultrasensitive detection of DNA hybridisation. Adv. Mat.26, 4838–4844. 10.1002/adma.201401084
113
LuC.LiuY.YingY.LiuJ. (2017). Comparison of MoS2, WS2, and graphene oxide for DNA adsorption and sensing. Langmuir33, 630–637. 10.1021/acs.langmuir.6b04502
114
MaD.HuangC.ZhengJ.TangJ.LiJ.YangJ.et al (2018). Quantitative detection of exosomal microRNA extracted from human blood based on surface-enhanced Raman scattering. Biosens. Bioelectron.101, 167–173. 10.1016/j.bios.2017.08.062
115
MajdS. M.SalimiA.GhasemiF. (2018). An ultrasensitive detection of miRNA-155 in breast cancer via direct hybridization assay using two-dimensional molybdenum disulfide field-effect transistor biosensor. Biosens. Bioelectron.105, 6–13. 10.1016/j.bios.2018.01.009
116
MarianiS.ScaranoS.SpadavecchiaJ.MinunniM. (2015). A reusable optical biosensor for the ultrasensitive and selective detection of unamplified human genomic DNA with gold nanostars. Biosens. Bioelectron.74, 981–988. 10.1016/j.bios.2015.07.071
117
MeiJ.LiY. T.ZhangH.XiaoM. M.NingY.ZhangZ. Y.et al (2018). Molybdenum disulfide field-effect transistor biosensor for ultrasensitive detection of DNA by employing morpholino as probe. Biosens. Bioelectron.110, 71–77. 10.1016/j.bios.2018.03.043
118
MinS. K.KimW. Y.ChoY.KimK. S. (2011). Fast DNA sequencing with a graphene-based nanochannel device. Nat. Nanotechnol.6, 162–165. 10.1038/nnano.2010.283
119
MineroG. A. S.CangianoV.GarbarinoF.FockJ.HansenM. F. (2019). Integration of microbead DNA handling with optomagnetic detection in rolling circle amplification assays. Mikrochim. Acta186, 528. 10.1007/s00604-019-3636-x
120
MineroG. A. S.TefikuE.GarbarinoF.FockJ.HansenM. F. (2020). On-chip DNA analysis of tuberculosis based on magnetic nanoparticle clustering induced by rolling circle amplification products. IEEE Magn. Lett.11, 1–5. 10.1109/LMAG.2019.2959545
121
MiscouridesN.YuL.-S.Rodriguez-ManzanoJ.GeorgiouP. (2018). A 12.8 k current-mode velocity-saturation ISFET array for on-chip real-time DNA detection. IEEE Trans. Biomed. Circuits Syst.12, 1202–1214. 10.1109/TBCAS.2018.2851448
122
MohammadiA. V.RosenJ.GogotsiY. (2021). The world of two-dimensional carbides and nitrides (MXenes). Science80-, eabf1581. 10.1126/science.abf1581
123
MoserN.Rodriguez-ManzanoJ.LandeT. S.GeorgiouP. (2018). A scalable ISFET sensing and memory array with sensor auto-calibration for on-chip real-time DNA detection. IEEE Trans. Biomed. Circuits Syst.12, 390–401. 10.1109/TBCAS.2017.2789161
124
MovilliJ.KolkmanR. W.RozziA.CorradiniR.SegerinkL. I.HuskensJ. (2020). Increasing the sensitivity of electrochemical DNA detection by a micropillar-structured biosensing surface. Langmuir36, 4272–4279. 10.1021/acs.langmuir.0c00144
125
MujawarM. A.GohelH.BhardwajS. K.SrinivasanS.HickmanN.KaushikA. (2020). Nano-enabled biosensing systems for intelligent healthcare: Towards COVID-19 management. Mat. Today Chem.17, 100306. 10.1016/j.mtchem.2020.100306
126
MustafaA. M.LiZ.ShaoL. (2017). “Molecular dynamics simulations of damage cascades creation in oxide-particle-embedded Fe,” in 25th International Conference on Nuclear Engineering,ASME, 1–5. 10.1115/ICONE25-67356
127
Mustafa AzeemM.Abd El GawadK.ZubairM.IbrahimS. A.AdoM.MehdiG. (2019). “Radiation damage effects in oxide dispersion strengthened steel alloys,” in Proceedings, ICONE International Conference on Nuclear Engineering. 10.1299/jsmeicone.2019.27.2086
128
Mustafa AzeemM.LiZ.WangQ.ZubairM. (2018). Molecular dynamics studies and irradiation effects in ODSS alloys. Int. J. Nucl. Energy Sci. Technol.12, 381–399. 10.1504/IJNEST.2018.097200
129
NaH.-K.WiJ.-S.SonH. Y.OkJ. G.HuhY.-M.LeeT. G. (2018). Discrimination of single nucleotide mismatches using a scalable, flexible, and transparent three-dimensional nanostructure-based plasmonic miRNA sensor with high sensitivity. Biosens. Bioelectron.113, 39–45. 10.1016/j.bios.2018.04.033
130
NapoliC.LaiS.GiannettiA.TombelliS.BaldiniF.BarbaroM.et al (2018). Electronic detection of DNA hybridization by coupling organic field-effect transistor-based sensors and hairpin-shaped probes. Sensors Switz.18, 990. 10.3390/s18040990
131
NareshV.LeeN. (2021). A review on biosensors and recent development of nanostructured materials-enabled biosensors. Sensors Switz.21, 1109–1135. 10.3390/s21041109
132
NgE.YaoC.ShultzT. O.Ross-HoweS.WangS. X. (2019). Magneto-nanosensor smartphone platform for the detection of HIV and leukocytosis at point-of-care. Nanomedicine16, 10–19. 10.1016/j.nano.2018.11.007
133
NguyenT. T. T.LegallaisM.MorisotF.CazimajouT.StambouliV.MouisM.et al (2019). First evidence of superiority of Si nanonet field effect transistors over multi-parallel Si nanowire ones in view of electrical DNA hybridization detection. Mat. Res. Express6, 016301. 10.1088/2053-1591/aae0d5
134
NovaraC.ChiadòA.PaccottiN.CatuognoS.EspositoC. L.CondorelliG.et al (2017). SERS-active metal-dielectric nanostructures integrated in microfluidic devices for label-free quantitative detection of miRNA. Faraday Discuss.205, 271–289. 10.1039/C7FD00140A
135
OuyangL.HuY.ZhuL.ChengG. J.IrudayarajJ. (2017). A reusable laser wrapped graphene-Ag array based SERS sensor for trace detection of genomic DNA methylation. Biosens. Bioelectron.92, 755–762. 10.1016/j.bios.2016.09.072
136
PalS.ChakrabortyM.SarkarN. (2020). Graphene oxide functionalized with 5-aminophenanthroline for selective detection of adenine through fluorescence “turn-off–on” response. ACS Appl. Nano Mat.3, 3532–3539. 10.1021/acsanm.0c00212
137
PatchingS. G. (2014). Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discovery. Biochim. Biophys. Acta - Biomembr.1838, 43–55. 10.1016/j.bbamem.2013.04.028
138
Perez-TorallaK.PereiroI.GarrigouS.Di FedericoF.ProudhonC.BidardF. C.et al (2019). Microfluidic extraction and digital quantification of circulating cell-free DNA from serum. Sensors Actuators, B Chem.286, 533–539. 10.1016/j.snb.2019.01.159
139
PloetzE.LernerE.HusadaF.RoelfsM.ChungS.HohlbeinJ.et al (2016). Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers. Sci. Rep.6, 1–18. 10.1038/srep33257
140
PradaJ.CordesC.HarmsC.LangW. (2019). Design and manufacturing of a disposable, cyclo-olefin copolymer, microfluidic device for a biosensor. Sensors Switz.19, 1178. 10.3390/s19051178
141
PramanikS.NandyA.ChakrabortyS.PramanikU.NandiS.MukherjeeS. (2020). Preferential binding of thioflavin T to AT-rich DNA: White light emission through intramolecular förster resonance energy transfer. J. Phys. Chem. Lett.11, 2436–2442. 10.1021/acs.jpclett.0c00237
142
QiX.BiJ. (2019). Plasmonic sensors relying on nanoparticle arrays created by a template-directed dewetting process. Opt. Commun.453, 124328. 10.1016/j.optcom.2019.124328
143
QianZ. S.ShanX. Y.ChaiL. J.MaJ. J.ChenJ. R.FengH. (2014). A universal fluorescence sensing strategy based on biocompatible graphene quantum dots and graphene oxide for the detection of DNA. Nanoscale6, 5671–5674. 10.1039/c3nr06583a
144
QuanP. L.SauzadeM.BrouzesE. (2018). Dpcr: A technology review. Sensors Switz.18, 1271. 10.3390/s18041271
145
RahmanM.CuiD.ZhouS.ZhangA.ChenD. (2020). A graphene oxide coated gold nanostar based sensing platform for ultrasensitive electrochemical detection of circulating tumor DNA. Anal. Methods12, 440–447. 10.1039/C9AY01620A
146
RamshaniZ.ZhangC.RichardsK.ChenL.XuG.StilesB. L.et al (2019). Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device. Commun. Biol.2, 189–9. 10.1038/s42003-019-0435-1
147
RenR.BiQ.YuanR.XiangY. (2020). An efficient, label-free and sensitive electrochemical microRNA sensor based on target-initiated catalytic hairpin assembly of trivalent DNAzyme junctions. Sensors Actuators, B Chem.304, 127068. 10.1016/j.snb.2019.127068
148
RoetherJ.ChuK.-Y.WillenbacherN.ShenA. Q.BhallaN. (2019). Real-time monitoring of DNA immobilization and detection of DNA polymerase activity by a microfluidic nanoplasmonic platform. Biosens. Bioelectron.142, 111528. 10.1016/j.bios.2019.111528
149
SaadS. M.AbdullahJ.Abd RashidS.FenY. W.SalamF.YihL. H. (2020). A carbon dots based fluorescence sensing for the determination of Escherichia coli O157:H7. Measurement160, 107845. 10.1016/j.measurement.2020.107845
150
Sánchez-PaniaguaM.Palenzuela-BatistaS.Manzanares-PalenzuelaC. L.López-RuizB. (2020). Electrochemical genosensor for Klotho detection based on aliphatic and aromatic thiols self-assembled monolayers. Talanta212, 120735. 10.1016/j.talanta.2020.120735
151
SanthanamM.AlgovI.AlfontaL. (2020). DNA/RNA electrochemical biosensing devices a future replacement of PCR methods for a fast epidemic containment. Sensors Switz.20, 1–15. 10.3390/s20164648
152
SeoG.LeeG.KimM. J.BaekS. H.ChoiM.KuK. B.et al (2020). Rapid detection of COVID-19 causative virus (SARS-CoV-2) in human nasopharyngeal swab specimens using field-effect transistor-based biosensor. ACS Nano14, 5135–5142. 10.1021/acsnano.0c02823
153
SforziJ.FerrautoG.AimeS.Geninatti CrichS. (2020). A simple and fast assay based on carboxyfluorescein-loaded liposome for quantitative DNA detection. ACS Omega5, 1764–1772. 10.1021/acsomega.9b01457
154
ShenC.-H. (2019). “Detection and analysis of nucleic acids,” in Diagnostic molecular biology. Editor ShenC.-H. B. T.-D. M. B. (Academic Press), 167–185. 10.1016/B978-0-12-802823-0.00007-9
155
ShenX.LiuD.ZhuC.LiY.LiuY.YouT. (2020). Photoelectrochemical and electrochemical ratiometric aptasensing: A case study of streptomycin. Electrochem. Commun.110, 106637. 10.1016/j.elecom.2019.106637
156
ShenZ.HeL.WangW.TanL.GanN. (2020). Highly sensitive and simultaneous detection of microRNAs in serum using stir-bar assisted magnetic DNA nanospheres-encoded probes. Biosens. Bioelectron.148, 111831. 10.1016/j.bios.2019.111831
157
ShlyapnikovY. M.MalakhovaE. A.ShlyapnikovaE. A. (2019). Rapid amplification-free microarray-based ultrasensitive detection of DNA. Anal. Chem.91, 11209–11214. 10.1021/acs.analchem.9b02149
158
SiY.XuL.WangN.ZhengJ.YangR.LiJ. (2020). Target MicroRNA-responsive DNA hydrogel-based surface-enhanced Raman scattering sensor arrays for MicroRNA-marked cancer screening. Anal. Chem.92, 2649–2655. 10.1021/acs.analchem.9b04606
159
SongG.ZhangR.JiangX.LiuF.LiuX. (2020a). Nitrogen-doped reduced graphene oxide as a sensing platform for detection of guanine and application in cell necrosis. Chem. Pap.74, 89–98. 10.1007/s11696-019-00856-0
160
SongR.TianM.LiY.LiuJ.LiuG. (2020b). Detection of MicroRNA based on three-dimensional graphene field-E ® ect transistor biosensor. Nano15, 1–8. 10.1142/S1793292020500393
161
ŠpačekJ.EksinE.HavranL.ErdemA.FojtaM. (2020). Fast enzyme-linked electrochemical sensing of DNA hybridization at pencil graphite electrodes. Application to detect gene deletion in a human cell culture. J. Electroanal. Chem.862, 113951. 10.1016/j.jelechem.2020.113951
162
StegmannU. E. (2005). Genetic information as instructional content. Philos. Sci.72, 425–443. 10.1086/498472
163
SunJ.XieX.XieK.XuS.JiangS.RenJ.et al (2019a). Magnetic graphene field-effect transistor biosensor for single-strand DNA detection. Nanoscale Res. Lett.14, 248–8. 10.1186/s11671-019-3048-1
164
SunY.CaiH.QiaoX.WangX. (2019b). High-performance polarization control modulated surface plasmon resonance sensor based on monolayer graphene/Au-NPs architecture for detection of DNA hybridization. Meas. Sci. Technol.30, 125701. 10.1088/1361-6501/ab383a
165
SunY.PengZ.LiH.WangZ.MuY.ZhangG.et al (2019c). Suspended CNT-Based FET sensor for ultrasensitive and label-free detection of DNA hybridization. Biosens. Bioelectron.137, 255–262. 10.1016/j.bios.2019.04.054
166
TadimetyA.ZhangY.KreadyK. M.PalinskiT. J.TsongalisG. J.ZhangJ. X. J. (2019). Design of peptide nucleic acid probes on plasmonic gold nanorods for detection of circulating tumor DNA point mutations. Biosens. Bioelectron.130, 236–244. 10.1016/j.bios.2019.01.045
167
TangS.LiY.ZhuA.YaoY.SunJ.ZhengF.et al (2019). A triple-amplification strategy based on the formation of peroxidase-like two-dimensional DNA/Fe3O4 networks initiated by the hybridization chain reaction for highly sensitive detection of microRNA. Chem. Commun.55, 8386–8389. 10.1039/C9CC03194D
168
TemizY.LovchikR. D.KaigalaG. V.DelamarcheE. (2015). Lab-on-a-chip devices: How to close and plug the lab?Microelectron. Eng.132, 156–175. 10.1016/j.mee.2014.10.013
169
TianB.HanY.FockJ.StrömbergM.LeiferK.HansenM. F. (2019). Self-Assembled magnetic nanoparticle-graphene oxide nanotag for optomagnetic detection of DNA. ACS Appl. Nano Mat.2, 1683–1690. 10.1021/acsanm.9b00127
170
TianM. (2018). RNA detection based on graphene field-effect transistor biosensor. Adv. Condens. Matter Phys., 27–32.
171
TianY.LuQ.GuoX.WangS.GaoY.WangL. (2020). Au nanoparticles deposited on ultrathin two-dimensional covalent organic framework nanosheets for in vitro and intracellular sensing. Nanoscale12, 7776–7781. 10.1039/C9NR08220D
172
TripathyS.JosephJ.PothuneediS.DasD.VanjariS. R. K.RaoA. V. S. S. N.et al (2019). A miniaturized electrochemical platform with an integrated PDMS reservoir for label-free DNA hybridization detection using nanostructured Au electrodes. Analyst144, 6953–6961. 10.1039/C9AN01076A
173
TrotterM.JuricD.BagherianZ.BorstN.GlaserK.MeissnerT.et al (2020). Inkjet-printing of nanoparticle gold and silver ink on cyclic olefin copolymer for DNA-sensing applications. Sensors (Basel)20, 1–15. 10.3390/s20051333
174
VerschuerenD. V.PudS.ShiX.De AngelisL.KuipersL.DekkerC. (2019). Label-free optical detection of DNA translocations through plasmonic nanopores. ACS Nano13, 61–70. 10.1021/acsnano.8b06758
175
VikrantK.BhardwajN.BhardwajS. K.KimK. H.DeepA. (2019). Nanomaterials as efficient platforms for sensing DNA. Biomaterials214, 119215. 10.1016/j.biomaterials.2019.05.026
176
WangH. N.RegisterJ. K.FalesA. M.GandraN.ChoE. H.BoicoA.et al (2018a). Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model. Nano Res.11, 4005–4016. 10.1007/s12274-018-1982-3
177
WangH.TangH.YangC.LiY. (2019a). Selective single molecule nanopore sensing of microRNA using PNA functionalized magnetic core–shell Fe3O4–Au nanoparticles. Anal. Chem.91, 7965–7970. 10.1021/acs.analchem.9b02025
178
WangJ.ZhaoW.-W.ZhouH.XuJ.-J.ChenH.-Y. (2013). Amplified electrochemiluminescence detection of DNA-binding protein based on the synergy effect of electron and energy transfer between CdS nanocrystals and gold nanoparticles. Biosens. Bioelectron.41, 615–620. 10.1016/j.bios.2012.09.041
179
WangQ.ZhangB.XuX.LongF.WangJ. (2018b). CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method. Sci. Rep.8, 1–13. 10.1038/s41598-018-32329-x
180
WangQ.ZouL.YangX.LiuX.NieW.ZhengY.et al (2019b). Direct quantification of cancerous exosomes via surface plasmon resonance with dual gold nanoparticle-assisted signal amplification. Biosens. Bioelectron.135, 129–136. 10.1016/j.bios.2019.04.013
181
WangX.ZhangJ.WeiY.XingT.CaoT.WuS.et al (2020). A copper-based metal–organic framework/graphene nanocomposite for the sensitive and stable electrochemical detection of DNA bases. Analyst145, 1933–1942. 10.1039/C9AN02398D
182
WangZ.JiaY. (2018). Graphene solution-gated field effect transistor DNA sensor fabricated by liquid exfoliation and double glutaraldehyde cross-linking. Carbon N. Y.130, 758–767. 10.1016/j.carbon.2018.01.078
183
WatsonJ. D.CrickF. H. C. (1953). Molecular structure of nucleic acids: A structure for deoxyribose nucleic acid. Nature171, 737–738. 10.1038/171737a0
184
WilkinsM. H. (1956). Physical studies of the molecular structure of deoxyribose nucleic acid and nucleoprotein. Cold Spring Harb. Symp. Quant. Biol.21, 75–90. 10.1101/sqb.1956.021.01.007
185
WuC.MangaY. B.YangM.ChienZ.LeeK. (2018). Label-free detection of BRAF V599E gene mutation using side-gated nanowire field effect transistors. J. Electrochem. Soc.165, B576–B581. 10.1149/2.0641813jes
186
WuC.ZhangX.WangR.ChenL. J.NieM.ZhangZ.et al (2022). Low-dimensional material based wearable sensors. Nanotechnology33, 072001. 10.1088/1361-6528/ac33d1
187
WuT.LiX.FuY.DingX.LiZ.ZhuG.et al (2020a). A highly sensitive and selective fluorescence biosensor for hepatitis C virus DNA detection based on δ-FeOOH and exonuclease III-assisted signal amplification. Talanta209, 120550. 10.1016/j.talanta.2019.120550
188
WuY.ChoiN.ChenH.DangH.ChenL.ChooJ. (2020b). Performance evaluation of surface-enhanced Raman scattering-polymerase chain reaction sensors for future use in sensitive genetic assays. Anal. Chem.92, 2628–2634. 10.1021/acs.analchem.9b04522
189
XiaoG.ChenB.HeM.LiX.HuB. (2019). A highly sensitive assay of DNA based on inductively coupled plasma mass spectrometry detection with gold nanoparticle amplification and isothermal circular strand-displacement polymerization reaction. Talanta202, 207–213. 10.1016/j.talanta.2019.05.018
190
XuM.FuP.XingS.ZhaoY.ZhaoC. (2020a). A PNA-DNA(2) triple-helix molecular switch-based colorimetric sensor for sensitive and specific detection of microRNAs from cancer cells. Chembiochem21, 2667–2675. 10.1002/cbic.202000155
191
XuS.ChangY.WuZ.LiY.YuanR.ChaiY. (2020b). One DNA circle capture probe with multiple target recognition domains for simultaneous electrochemical detection of miRNA-21 and miRNA-155. Biosens. Bioelectron.149, 111848. 10.1016/j.bios.2019.111848
192
YadavP.CaoZ.Barati FarimaniA. (2021). DNA detection with single-layer Ti3C2MXene nanopore. ACS Nano15, 4861–4869. 10.1021/acsnano.0c09595
193
YangB.KongJ.FangX. (2019). Bandage-like wearable flexible microfluidic recombinase polymerase amplification sensor for the rapid visual detection of nucleic acids. Talanta204, 685–692. 10.1016/j.talanta.2019.06.031
194
YeH.DeS. (2017). Thermal injury of skin and subcutaneous tissues: A review of experimental approaches and numerical models. J. Int. Soc. Burn Inj.43, 909–932. 10.1016/j.burns.2016.11.014
195
YiJ. C.KimY. J.KimY. Y.LeeS.WooD. H.KimC. (2019). “Detection of single-stranded DNA using the Dirac voltage change of graphene-based FETs,” in IEEE Region 10 Annual International Conference, Proceedings/TENCON, 2051–2053. 10.1109/TENCON.2019.8929535
196
YinZ.CuiH.ShuQ.JinC.LinY.SuJ.et al (2020). Multi-signal amplification electrochemical DNA biosensor based on exonuclease III and tetraferrocene. J. Mat. Chem. B8, 4143–4150. 10.1039/D0TB00204F
197
YouL.LiR.DongX.WangF.GuoJ.WangC. (2017). Micron-sized surface enhanced Raman scattering reporter/fluorescence probe encoded colloidal microspheres for sensitive DNA detection. J. Colloid Interface Sci.488, 109–117. 10.1016/j.jcis.2016.10.086
198
ZhangD.YanY.QueH.YangT.ChengX.DingS.et al (2020). CRISPR/Cas12a-Mediated interfacial cleaving of hairpin DNA reporter for electrochemical nucleic acid sensing. ACS sensors5, 557–562. 10.1021/acssensors.9b02461
199
ZhangH.FuC.WuS.ShenY.ZhouC.NengJ.et al (2019a). Magnetic-capture-based SERS detection of multiple serum microRNA biomarkers for cancer diagnosis. Anal. Methods11, 783–793. 10.1039/C8AY02423E
200
ZhangH. W.ZhangL.SunX.ZhangZ. R. (2007). Successful transfection of hepatoma cells after encapsulation of plasmid DNA into negatively charged liposomes. Biotechnol. Bioeng.96, 118–124. 10.1002/bit.21146
201
ZhangJ.BaY.LiuQ.ZhaoL.WangD.YangH.et al (2020). CuBr(2)/EDTA-mediated ATRP for ultrasensitive fluorescence detection of lung cancer DNA. J. Adv. Res.22, 77–84. 10.1016/j.jare.2019.11.006
202
ZhangJ.HuS.WangZ.JiaY. (2019b). Detection of methylated cytosine on single strand DNA based on immune-functionalized and glutaraldehyde modified liquid exfoliated graphene field effect transistor. Proc. SPIE, 113841D. 10.1117/12.2559509
203
ZhangJ.SongC.ZhouH.JiaJ.DaiY.CuiD.et al (2020d). A dual signal amplification strategy for the highly sensitive fluorescence detection of nucleic acids. Analyst145, 1219–1226. 10.1039/c9an02183c
204
Zhang YY.HaoL.ZhaoZ.YangX.WangL.LiuS. (2020). Immuno-DNA binding directed template-free DNA extension and enzyme catalysis for sensitive electrochemical DNA methyltransferase activity assay and inhibitor screening. Analyst145, 3064–3072. 10.1039/D0AN00008F
205
ZhangZ.PettinariR.ZhouL.SongY.WangM.HeL.et al (2020e). Sensors and Actuators B: Chemical Construction of the 0D/2D heterojunction of Ti 3 C 2 T x MXene nanosheets and iron phthalocyanine quantum dots for the impedimetric aptasensing of. Sensors Actuators B. Chem.310, 127844. 10.1016/j.snb.2020.127844
206
ZhaoL.KongD.WuZ.LiuG.GaoY.YanX.et al (2020). Interface interaction of MoS2 nanosheets with DNA based aptameric biosensor for carbohydrate antigen 15–3 detection. Microchem. J.155, 104675. 10.1016/j.microc.2020.104675
207
ZhengX.ZhaoL.WenD.WangX.YangH.FengW.et al (2020). Ultrasensitive fluorescent detection of HTLV-II DNA based on magnetic nanoparticles and atom transfer radical polymerization signal amplification. Talanta207, 120290. 10.1016/j.talanta.2019.120290
208
ZhouW.HanY.BeliveauB. J.GaoX. (2020). Combining qdot nanotechnology and DNA nanotechnology for sensitive single-cell imaging. Adv. Mat.32, 1908410. 10.1002/adma.201908410
209
ZhouX.ZhuD.LiaoY.LiuW.LiuH.MaZ.et al (2014). Synthesis, labeling and bioanalytical applications of a tris(2,2’-bipyridyl)ruthenium(II)-based electrochemiluminescence probe. Nat. Protoc.9, 1146–1159. 10.1038/nprot.2014.060
210
ZhouY.JiangW.WuH.LiuF.YinH.LuN.et al (2019). Amplified electrochemical immunoassay for 5-methylcytosine using a nanocomposite prepared from graphene oxide, magnetite nanoparticles and β-cyclodextrin. Mikrochim. Acta186, 488. 10.1007/s00604-019-3575-6
211
ZiółkowskiR.OszwałdowskiS.KopyraK. K.ZacharczukK.ZasadaA. A.MalinowskaE. (2020). Toward Fluorimetric-Paired-Emitter-Detector-Diode test for Bacillus anthracis DNA based on graphene oxide. Microchem. J.154, 104592. 10.1016/j.microc.2019.104592
212
ZopfD.PittnerA.DatheA.GrosseN.CsákiA.ArstilaK.et al (2019). Plasmonic nanosensor array for multiplexed dna-based pathogen detection. ACS Sensors4, 335–343. 10.1021/acssensors.8b01073
Summary
Keywords
detection, deoxyribonucleic acid, sensors, polymerase chain reaction, surface plasmon resonance, field effect transistor, nucleotide sensors
Citation
Azeem MM, Shafa M, Aamir M, Zubair M, Souayeh B and Alam MW (2023) Nucleotide detection mechanism and comparison based on low-dimensional materials: A review. Front. Bioeng. Biotechnol. 11:1117871. doi: 10.3389/fbioe.2023.1117871
Received
07 December 2022
Accepted
13 February 2023
Published
02 March 2023
Volume
11 - 2023
Edited by
Jeong-Hwan Kim, Yokohama City University, Japan
Reviewed by
Weiling Song, Qingdao University of Science and Technology, China
Quanjiang Dong, Qingdao University Medical College, China
Updates
Copyright
© 2023 Azeem, Shafa, Aamir, Zubair, Souayeh and Alam.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: M. Mustafa Azeem, mustafa@mst.edu; Muhammad Aamir, msadiq@kfu.edu.sa
This article was submitted to Biosensors and Biomolecular Electronics, a section of the journal Frontiers in Bioengineering and Biotechnology
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