PERSPECTIVE article

Front. Bioeng. Biotechnol.

Sec. Cell and Gene Therapy

Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1569298

Challenges in lentiviral vector production: retro-transduction of producer cell lines

Provisionally accepted
Maximilian KlimpelMaximilian Klimpel1*Christin ObertChristin Obert1Monica TerraoMonica Terrao1Parameswari SinghParameswari Singh1Haiyan WeiHaiyan Wei2Chao-Guang ChenChao-Guang Chen2Andreas GilleAndreas Gille3Silke WissingSilke Wissing1Holger LauxHolger Laux1
  • 1CSL Innovation GmbH, Marburg, Germany
  • 2CSL Limited, Parkville, Australia
  • 3CSL Behring, Pasadena, United States

The final, formatted version of the article will be published soon.

Select one of your emails

You have multiple emails registered with Frontiers:

Notify me on publication

Please enter your email address:

If you already have an account, please login

You don't have a Frontiers account ? You can register here

The authors gratefully acknowledge the support by team members of the VVPD, VVB and Research department of CSL Innovation GmbH.Klimpel drafted the original manuscript and is responsible for revisions based on reviewer feedback. Maximilian Klimpel planned and executed experiments for lentivirus productions. Christin Obert, Silke Wissing and Monica Terrao developed the analytical technologies for determination of retro-transduction. Christin Obert and Monica Terrao executed experiments for determination of retro-transduction. Andreas Gille developed the ENV-Y concept. Chao-Guang Chen developed the concepts for LDLR gene knockouts of GPRG-derived cell lines and Haiyan Wei executed experiments for LDLR gene knockouts. Holger Laux and Parameswari Govindarajan contributed to the conception of experiments related to retro-transduction.

Keywords: lentiviral vector (LV), Viral vector production, Retro-transduction, Auto-transduction, cell and gene therapy (CGT), stable producer cell line, LDL-R knockout, VSV-G (vesicular stomatitis virus G glycoprotein) pseudotyped HIV-1 derived vector particles

Received: 31 Jan 2025; Accepted: 05 May 2025.

Copyright: © 2025 Klimpel, Obert, Terrao, Singh, Wei, Chen, Gille, Wissing and Laux. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Maximilian Klimpel, CSL Innovation GmbH, Marburg, Germany

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Supplementary Material