ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Bioprocess Engineering
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1611313
This article is part of the Research TopicDesign Strategies and Equipment Requirements for Efficient Process Development and Robust Manufacturing of Cell TherapiesView all 7 articles
Enhanced Cancer Cell Sorting Using Lab-on-a-Disc Pattern Design with Magnetic and Centrifugal Forces
Provisionally accepted
- 1National Chung Hsing University, Taichung, Taiwan
- 2National Chin-Yi University of Technology, Taiping City, Taiwan
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Using the characteristic of microfluidic flow for biological detection is a non-invasive method that can replace traditional invasive testing methods to achieve fast and accurate results. The design of the detection device and Lab-on-a-Disk (LoaD) can impact the performance in identifying biological features and the accuracy. Therefore, we created a novel device to extract cancer cells from a heterogeneous cell population by centrifugal force-driven microfluidic flow, and magnetic labeling in this study. Two-stage centrifugal force and the specially designed LoaD were used to drive the microfluidic flow and control the microfluidic movement to the designated areas. The purpose was to allow CD44 antibody-magnetic bead complex (CD44 beads), which specifically bind to the abundantly present CD44 receptors on identifiable cancer cells, to flow into the reservoir well, while the biological mixture containing the cancer cells is retained in the capture well. Fluorescence imaging as well as flow cytometric analysis revealed successful retention of the microbead-bound cancer cells in the magnetic area, while the remaining biological mixture was retained in the reservoir area. Given that the entire separation process took less than 2 hours.
Keywords: Microfluidic flow, cell sorting, non-invasive method, Lab-on-a-disc, Rapid detection
Received: 14 Apr 2025; Accepted: 09 Jul 2025.
Copyright: © 2025 Cheng, Chao, Chen and Lin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yao-Tsung Lin, National Chin-Yi University of Technology, Taiping City, Taiwan
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