ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1666265
Lyophilized Horizontal Platelet Rich Fibrin Promotes the healing of Infected Burns/Wounds by Modulating Macrophage Polarization and Fibroblast Migration
Provisionally accepted
Jihua Chai1,2,3
Detian Miao4
Richard J Miron5Shuo Xu1,2,3Xiaoxin Zhang1,2,3
Yulan Wang1,2,3Yi Bai1,2,3*- 1Department of Oral Medicine, School of Stomatology, Wuhan University, Wuhan, Hebei Province, China
- 2Key Laboratory of Oral Biomedical Education, Hospital of Stomatology, Faculty of Medical Sciences, Wuhan University, Wuhan, Hubei Province, China
- 3Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hebei Province, China
- 4Binzhou Medical University Hospital, Binzhou, China
- 5Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Bern, Switzerland
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BACKGROUND: The treatment of infected burns is a major clinical challenge.Platelet-rich fibrin (PRF) made by horizontal centrifugation has antibacterial and tissue repair properties. Its freeze-dried form (Ly-H-PRF) is easy to store and may help heal infected burns. This study explores the healing effects and mechanisms of Ly-H-PRF in infected burns. METHODS: Blood from healthy volunteers was used to make Ly-H-PRF. Its antibacterial effects against Staphylococcus aureus and Escherichia coli were tested using the spread plate method. Ly-H-PRF's effects on macrophage cell cycle and polarization were examined using flow cytometry and fluorescence staining. Its therapeutic effects on infected burns were evaluated in a mouse model of second-degree burns.RESULTS: Ly-H-PRF significantly inhibited the growth of S. aureus and E. coli , protected macrophages and fibroblasts from apoptosis, reduced M1 polarization, and promoted M2 polarization. It also enhanced fibroblast migration. In the mouse model, Ly-H-PRF treatment improved burn healing compared to conventional ointment, with less inflammation and more collagen synthesis. CONCLUSION: Ly-H-PRF effectively promotes infected burn healing through antibacterial properties, macrophage regulation, and fibroblast migration, offering a potential economical and convenient treatment option.
Keywords: Lyophilized, horizontal centrifugation, Platelet rich fibrin, Infected Burned Wound, macrophage
Received: 17 Jul 2025; Accepted: 02 Sep 2025.
Copyright: © 2025 Chai, Miao, Miron, Xu, Zhang, Wang and Bai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yi Bai, Department of Oral Medicine, School of Stomatology, Wuhan University, Wuhan, 430079, Hebei Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.