ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Tissue Engineering and Regenerative Medicine
Establishment and characterization of a human juvenile bone marrow-derived mesenchymal stem/stromal cell line under advanced culture conditions for osteogenic differentiation
Provisionally accepted
Julia Moldaschl1
Sofia Danilchenko1
Isita Sagar1
Vivian-Pascal Brandt2Heidrun Holland2
Stephan Handschuh3
Martin Glösmann3
Stefan Toegel4,5
Tobias May6
Dominik Egger7*
Cornelia Kasper1*- 1Universitat fur Bodenkultur Wien, Vienna, Austria
- 2Universitat Leipzig, Leipzig, Germany
- 3Veterinarmedizinische Universitat Wien, Vienna, Austria
- 4Medizinische Universitat Wien, Vienna, Austria
- 5Ludwig Boltzmann Gesellschaft, Vienna, Austria
- 6InSCREENeX GmbH, Braunschweig, Germany
- 7Leibniz University Hannover, Hanover, Germany
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Human mesenchymal stem/stromal cells (MSC) from juvenile donors (juvMSC) are crucial for studying bone development and for modeling pediatric skeletal diseases. However, the limited availability of these cells and the lack of physiologically relevant in vitro models hinder preclinical research. To address these issues, we established and characterized a new human bone marrow-derived MSC line under advanced culture conditions. Primary MSC from a 12-year-old donor in good health were immortalized via lentiviral transduction using a library of expansion genes. The resulting clone, C15 juvMSC, retained key features of MSC, including typical morphology, high proliferation rate, expression of stemness surface markers, and trilineage differentiation in a 3D format. Spectral karyotyping confirmed genomic stability without chromosomal aberrations. In 3D spheroid cultures, the C15 juvMSC demonstrated strong osteogenic potential, as evidenced by mineralization and alkaline phosphatase (ALP) activity. However, they exhibited a distinct differentiation pattern compared to primary cells. Overall, the C15 juvMSC line exhibits stable, scalable, and physiologically relevant characteristics, making it a valuable model for studying osteogenesis and for in vitro research on pediatric bone disorders.
Keywords: 3Dculture, Cell Line, juvenile, Mesenchymal stem/stromal cell, Osteogenic differentiation
Received: 06 Oct 2025; Accepted: 09 Dec 2025.
Copyright: © 2025 Moldaschl, Danilchenko, Sagar, Brandt, Holland, Handschuh, Glösmann, Toegel, May, Egger and Kasper. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dominik Egger
Cornelia Kasper
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