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CORRECTION article

Front. Bioeng. Biotechnol., 22 January 2026

Sec. Tissue Engineering and Regenerative Medicine

Volume 13 - 2025 | https://doi.org/10.3389/fbioe.2025.1737950

Correction: A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model

This article is a correction to:

  1. A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model

    1. Read original article
Monica MarzagalliMonica Marzagalli1Giorgia PelizzoniGiorgia Pelizzoni2Arianna FediArianna Fedi3Chiara Vitale,Chiara Vitale3,4Fabrizio FontanaFabrizio Fontana5Silvia BrunoSilvia Bruno4Alessandro PoggiAlessandro Poggi6Alessandra Dondero,Alessandra Dondero4,7Maurizio AielloMaurizio Aiello3Roberta Castriconi,Roberta Castriconi4,7Cristina Bottino,Cristina Bottino4,7Silvia Scaglione Silvia Scaglione3*
  • 1React4life S.r.l, Genoa, Italy
  • 2Department of Biotechnology and Bioscience, University of Milano-Bicocca, Piazza Della Scienza, Milan, Italy
  • 3National Research Council, CNR-IEIIT, Genoa, Italy
  • 4Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy
  • 5Department of Pharmacological and Biomolecular Sciences (DiSFeB), University of Milan, Milan, Italy
  • 6Molecular Oncology and Angiogenesis Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy
  • 7IRCCS Istituto Giannina Gaslini, Genoa, Italy

A Correction on
A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model

by Marzagalli M, Pelizzoni G, Fedi A, Vitale C, Fontana F, Bruno S, Poggi A, Dondero A, Aiello M, Castriconi R, Bottino C and Scaglione S (2022) Front. Bioeng. Biotechnol. 10:945149. doi: 10.3389/fbioe.2022.945149

A correction has been made to the section Results, Dynamic culture and assessment of natural killer cell “extravasation”, Paragraph 3:

The authors described that they used 40% FBS as a chemoattractant: “To this purpose, additional experimental groups included 1) a positive control of cell migration (“empty” 3D alginate scaffolds, without tumor cells, in culture medium enriched with 40% FBS as a chemoattractant), and 2) a negative control (without alginate scaffolds, with culture medium without any chemoattracting supplement).”

However, the authors used 30%. The corrected paragraph is:

“To this purpose, additional experimental groups included 1) a positive control of cell migration (“empty” 3D alginate scaffolds, without tumor cells, in culture medium enriched with 30% FBS as a chemoattractant), and 2) a negative control (without alginate scaffolds, with culture medium without any chemoattracting supplement).”

A correction has been made to the section Results, Dynamic culture and assessment of natural killer cell “extravasation”, Paragraph 3:

“the stimulation does not report cells “extravasation” events from the bottom chamber towards the tumor, because of the fluid motion alone, as shown in Figure 6C.”

“Figure 6C” should be “Figure 6A.” The corrected paragraph is:

“the stimulation does not report cells “extravasation” events from the bottom chamber towards the tumor, because of the fluid motion alone, as shown in Figure 6A”.

A correction has been made to the section Results, Natural killer cell infiltration, Paragraph 1:

“Figure 6D” is mentioned incorrectly and should be changed to “Figure 6C.” The corrected paragraph is:

“After co-culturing the cells for 4 h under microcirculation, the 3D alginate cultures were recovered, fixed with 4% PFA, NK cells stained for the DNAM-1 marker (Castriconi et al., 2004), and observed under a fluorescence microscopy. As shown in Figure 6C, DNAM-1+ cells were mostly located along the border of the gels. This is indicative of the ability of NK cells not only to specifically migrate toward the tumor culture, but also to infiltrate the alginate matrix.”

The original article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: immune-organ-on-chip, 3D human tumor model, natural killer cells, neuroblastoma, cells migration, cells infiltration

Citation: Marzagalli M, Pelizzoni G, Fedi A, Vitale C, Fontana F, Bruno S, Poggi A, Dondero A, Aiello M, Castriconi R, Bottino C and Scaglione S (2026) Correction: A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model. Front. Bioeng. Biotechnol. 13:1737950. doi: 10.3389/fbioe.2025.1737950

Received: 02 November 2025; Accepted: 17 December 2025;
Published: 22 January 2026.

Edited and reviewed by:

George Alexander Truskey, Duke University, United States

Copyright © 2026 Marzagalli, Pelizzoni, Fedi, Vitale, Fontana, Bruno, Poggi, Dondero, Aiello, Castriconi, Bottino and Scaglione. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Silvia Scaglione, c2lsdmlhLnNjYWdsaW9uZUBjbnIuaXQ=

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.