Injectable Electroconductive Prussian Blue Nanofiber-PVA Hydrogel Modulates the Wound Microenvironment to Promote Diabetic Wound Healing

Dianqing Wang¹Jason Jone²Jin Huang^1*Sheng Chang^1*

• ¹First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
• ²Swansea University, Swansea, United Kingdom

Chronic diabetic wounds exhibit persistent oxidative stress, prolonged inflammation, impaired angiogenesis, and a disrupted bioelectric microenvironment that hinders re-epithelialization. Here, we develop an injectable Prussian Blue nanofiber-PVA hydrogel (PBM.PVA gel) with electroconductive and immunomodulatory features for accelerated diabetic wound repair. Electrospun PBM nanofibers were uniformly embedded within a physically cross linked PVA matrix, producing a flexible and adhesive composite with stable conductivity. In vitro, PBM.PVA gel showed excellent cytocompatibility, reduced pro-inflammatory cytokines (IL-6, TNF-α, CD86), and enhanced pro-regenerative markers (CD206, CD31). In streptozotocin-induced diabetic mice, the hydrogel significantly accelerated wound closure, reduced inflammatory 1Corresponding author: Sheng Chang TEL/FAX: +86 0416-4197717 E-mail: 324072062@qq.com #These authors contributed equally as corresponding authors: Jin Huang (E-mail: huangjin@jzmu.edu.cn) infiltration, and promoted collagen deposition with increased CD31-positive staining. While PBM has been reported to possess redox-regulatory potential, ROS levels and endogenous wound electrical fields were not directly quantified in this study; therefore, mechanistic interpretations are described as plausible and require further validation. Together, PBM.PVA gel provides a multifunctional dressing that supports a favorable wound microenvironment and improves healing outcomes in diabetic wounds.