ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Tissue Engineering and Regenerative Medicine
Efficacy of the Combination of Umbilical Fat and Venous Blood on Limb Salvage
Provisionally accepted
- Gaziantep Universitesi Tip Fakultesi, Gaziantep, Türkiye
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
SUMMARY OBJECTIVE: To evaluate the efficacy of combined umbilical adipose tissue and venous blood– derived stromal vascular fraction (SVF) on limb perfusion, pain and functional recovery in patients with chronic limb-threatening ischemia (CLTI). METHODS: A total of 20 patients aged between 36 and 82 years (mean age: 60.3 ± 11.2 years) were included in the study. Ankle-Brachial Index (ABI), pain scores and pain-free walking distance were evaluated before treatment and at follow-up at 3, 6, 9 and 12 months. RESULTS: At 12 months, ABI increased significantly, Visual Analog Scale (VAS) decreased, while pain-free walking distance improved at 9 and 12 months compared to baseline. Left ventricular ejection fraction (LVEF) showed a non-significant rise (54.6% to 55.2%, p = 0.072), while renal function improved modestly (creatinine 1.12 → 1.09 mg/dL, eGFR 78 → 80.5 mL/min/1.73 m², p = 0.034). Angiography revealed enhanced vessel visualization and collateral formation. CONCLUSIONS: Combined Autologous conditioned plasma (ACP) and adipose-derived SVF injections effectively improved limb perfusion, pain, lesion severity and functional outcomes in CLTI patients, suggesting a promising therapeutic approach for peripheral arterial disease (PAD).
Keywords: Autologous conditioned plasma, chronic limb-threatening ischemia, Limb Salvage, Stromal vascular fraction, Umblical Fat
Received: 10 Jan 2026; Accepted: 16 Feb 2026.
Copyright: © 2026 Hafız. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Erhan Hafız
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.