ORIGINAL RESEARCH article
Front. Cardiovasc. Med.
Sec. Atherosclerosis and Vascular Medicine
Volume 12 - 2025 | doi: 10.3389/fcvm.2025.1578711
Microarray profile of circular RNAs identifies CBT15_circR_28491 and T helper cell as new regulators for deep vein thrombosis
Provisionally accepted
- Shanghai General Hospital, Shanghai, China
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Background: Deep vein thrombosis (DVT) is the third most common cardiovascular disorder can lead to high mortality and morbidity. This study aimed to clarify the molecular and immune characteristics of circular RNAs (circRNAs) and mRNAs in DVT progression.Methods: DVT-associated dataset GSE148333 was downloaded from NCBI GEO.to screen differentially expressed circRNAs and mRNAs using limma package. Then, DVT-related modules/genes were filtered using WGCNA, and then important hub genes related to DVT were obtained by the four algorithms, including MCC, MNC, EPC and DEGRE. Afterwards, a circRNAs-miRNAs-hub genes network was constructed, and the correlation of the DVT-related important hub genes and immunity were analyzed. Finally, a DVT rat model was established to verify the expression of crucial circRNAs and hub genes by RT-qPCR.Results: A total of 421 circRNA and 1082 mRNAs were differentially expressed in DVT, and then 235 DVT-related circRNAs and 207 DVT-related mRNAs, which significantly enriched in the signaling pathways of NOD-like receptor, mTOR, FoxO, p53, and cell cycle. Thereafter, 17 important hub genes were obtained, including Birc5,
Keywords: deep vein thrombosis, circular RNAs, CirRNAs-miRNA-hub genes network, immune cells, Hub genes
Received: 18 Feb 2025; Accepted: 30 May 2025.
Copyright: © 2025 Chen, Zhu, Niu, Chang and Gan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shujie Gan, Shanghai General Hospital, Shanghai, 201620, China
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