Severe Attenuation of Circadian Clock Output in the Heart following Sustained Augmentation of Cardiomyocyte Protein O-GlcNAcylation First Author's Surname: Shanmugam

Gobinath Shanmugam¹Samuel F Chang¹Helen E Collins²Chae-Myeong Ha¹Mariame Selma KANE¹Luke Potter¹Lily Xie¹Luyun Zou¹Jianhua Zhang¹John C Chatham¹Adam R. Wende, Ph.D.¹Martin Young^1*

• ¹University of Alabama at Birmingham, Birmingham, United States
• ²School of Medicine, University of Louisville, Louisville, Kentucky, United States

Background. Changes in circadian-related behaviors (e.g., the timing of food intake, sleep cycles) and the environment (e.g., light-dark cycles) increase the risk of numerous cardiometabolic diseases, including diabetes mellitus and cardiac disease. Recent studies indicate a close interrelationship between circadian clocks and the posttranslational modification, protein O-GlcNAcylation. The current study was designed to investigate whether a modest elevation of protein O-GlcNAcylation in the adult mouse heart, similar to levels observed during pathologic states, influenced circadian governance of the heart.. Cardiomyocyte-specific expression of a dominant negative O-GlcNAcase (dnOGAh) for a 2-week period resulted in an approximate 1.5-fold increase in cardiac protein O-GlcNAcylation, impacting 70% of core circadian clock components in the heart at the mRNA level. Further interrogation of cardiac mRNA species in dnOGAh hearts at candidate (RT-PCR) and unbiased (RNAseq) levels revealed a 95% loss of circadian governance of the cardiac transcriptome. This was despite persistent/augmented 24hr oscillations of the core circadian clock proteins BMAL1, REVERBa, and PER2 in dnOGAh hearts. Direct comparison of dnOGAh hearts with cardiomyocyte-specific BMAL1 knockout (CBK) hearts underscored an apparent uncoupling of the core clock mechanism from clock control of downstream target genes in dnOGAh hearts, and highlighted that loss of circadian governance results in interstitial fibrosis.Conclusions. Sustained protein O-GlcNAcylation in the heart causes loss of circadian governance, likely downstream of the core circadian clock mechanism. Moreover, interstitial fibrosis appears to be a universal adverse outcome following impaired circadian governance.