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ORIGINAL RESEARCH article

Front. Cardiovasc. Med.

Sec. Pediatric Cardiology

Multimodal Evidence Chain of Iron Overload, Inflammation, and Dysfunction: An Integrated Predictive Model of Early Cardiac Injury in Pediatric Transfusion-Dependent β-Thalassemia

    PZ

    Panyan Zhou

    CL

    Caili Li

    XG

    Xiaomei Gao

    CY

    Caifen Ye

    MH

    Mufang Huang

    HZ

    Heng Zhang

  • Zhuhai People's Hospital, Zhuhai, China

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Abstract

Introduction: Despite standardized transfusion and chelation therapy, children with transfusion-dependent β-thalassemia (TDT) remain at high risk for cardiac dysfunction due to iron overload. Conventional ejection fraction assessment lacks sensitivity for early injury. This study evaluated multimodal indicators to develop a robust early-warning model. Methods: A prospective cohort of 128 TDT children (3–16 years) underwent cardiac magnetic resonance (CMR) T2* imaging, echocardiography with global longitudinal strain (GLS), and measurement of circulating biomarkers including high-sensitivity cardiac troponin I (hs-cTnI), B-type natriuretic peptide (BNP), interleukin-6, and tumor necrosis factor-α. Children were classified into dysfunction and normal groups based on LVEF and GLS. Logistic regression identified predictors, and ROC analysis validated the integrated model. Results: The dysfunction group demonstrated reduced GLS, ventricular remodeling, elevated hs-cTnI and BNP, and significantly shorter T2* values compared with controls (p < 0.001). Inflammatory cytokines were also upregulated. Multivariate analysis identified hs-cTnI, BNP, and T2* as independent predictors. The combined three-factor model achieved excellent discrimination (AUC 0.914), outperforming single markers, with preserved calibration following bootstrap validation. Conclusion: By linking iron overload, myocardial injury, inflammation, and structural dysfunction, this study proposes a clinically feasible integrated model for early cardiac risk detection in pediatric TDT. The approach supports precision monitoring and prevention of heart failure.

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Keywords

B-TypeNatriuretic Peptide, Cardiac dysfunction, Iron Overload Mechanism, Transfusion-dependent β-thalassemia, Troponin I

Received

30 September 2025

Accepted

06 January 2026

Copyright

© 2026 Zhou, Li, Gao, Ye, Huang and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Panyan Zhou

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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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