ORIGINAL RESEARCH article

Front. Cell Dev. Biol.

Sec. Cellular Biochemistry

Volume 13 - 2025 | doi: 10.3389/fcell.2025.1572571

This article is part of the Research TopicMolecular Mechanisms and Precision Medicine in Rare DiseasesView all articles

Establishment of human periodontal ligament cell lines with ALPL mutations to mimic dental aspects of hypophosphatasia

Provisionally accepted
  • 1University Hospital Würzburg, Würzburg, Bavaria, Germany
  • 2Julius Maximilian University of Würzburg, Würzburg, Germany
  • 3University of Belgrade, Belgrade, Serbia

The final, formatted version of the article will be published soon.

Besides skeletal symptoms, dental abnormalities are a typical feature of the rare inherited disorder hypophosphatasia (HPP), which is caused by loss of function mutations in the ALPL gene (alkaline phosphatase, biomineralization associated) coding for tissue-nonspecific alkaline phosphatase (TNAP). Dental symptoms include premature loss of deciduous teeth, disturbance in dentin and cementum mineralization, and an increased risk for periodontitis. However, the underlying molecular mechanisms are not fully understood and experimental cell lines for in vitro analyses of these processes are missing.We aimed to develop a physiologically relevant cellular model of dental origin with genetic ALPL variants to investigate the molecular consequences of TNAP deficiencies in vitro. For this purpose, we used immortalized periodontal ligament stem cells (PDL-hTERT cells) to establish five independent clonal cell lines via CRISPR/Cas9, harboring different ALPL genetic variants. Detailed investigation of their genetic properties revealed that four different genotypes were successfully established at two different positions within the ALPL gene locus. The detected variants either result in mis-splicing of ALPL mRNAs or in frameshift mutations. All determined variants implied severe consequences on TNAP function, as indicated by in silico modeling and comparison to reported human pathogenic variants. Subsequent detailed cell culture experiments demonstrated TNAP residual gene expression and altered TNAP activity in the newly established ALPL tg PDL-hTERT lines. Further assessment of cell line features showed significantly reduced cell growth, partly lower levels of intracellular ATP as well as mitochondrial function proteins. TNAP activity was furthermore investigated during in vitro osteogenic differentiation and strong suppression during this process in nearly all newly established lines was observed. Thereby, we report the generation of a new set of immortalized ALPL tg PDL-hTERT cells for investigation of TNAP cellular function in PDL cells, which can be used in subsequent studies for deciphering molecular processes in dental cells affected by reduction of TNAP function.

Keywords: Hypophosphatasia, ALPL, TNAP, PDL-hTERT cells, in vitro Article type: Original Research

Received: 07 Feb 2025; Accepted: 19 May 2025.

Copyright: © 2025 Schiffmaier, Rehling, Marnet, Borst, Trivanović, Docheva, Jakob, Graser, Herrmann and Liedtke. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Daniel Liedtke, Julius Maximilian University of Würzburg, Würzburg, Germany

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