ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Stem Cell Research
Volume 13 - 2025 | doi: 10.3389/fcell.2025.1627220
This article is part of the Research TopicAdvances in Dental Pulp Stem Cell Biology and ApplicationsView all 5 articles
Dental Pulp Stem Cells Conditioned Medium-Functionalized Microspheres for Endodontic Regeneration
Provisionally accepted
- 1Tianjin Stomatological Hospital,Tianjin,China, Tianjin, China
- 2Tianjin Hospital, Tianjin, China
- 3Department of Minimally Invasive Spine Surgery, Tianjin Hospital, Tianjin University, Department of Minimally Invasive Spine Surgery, Tianjin Hospital, Tianjin University, China
- 4Stomatology Department, Tianjin Ninghe District Qilihai Hospital, Stomatology Department, Tianjin Ninghe District Qilihai Hospital, Tianjin, China
- 5Stomatology Department, Tianjin Ninghe District Qilihai Hospital, Tianjin, China
- 6Stomatology Department, Tianjin Ninghe District Qilihai Hospital, Tianjin Ninghe District Qilihai Hospital, China
- 7Stomatology Department, Tianjin Ninghe District Qilihai Hospital, Tianjin Ninghe District, China
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Dental pulp disease is a prevalent oral pathology, yet functional regeneration of the pulp-dentin complex remains a significant challenge. Recent advancements in stem cell-based endodontic regenerative therapy have garnered substantial interest. This approach adheres to the foundational principles of tissue engineering, involving the integration of stem cells, scaffold materials, and growth factors within the root canal to achieve functional pulp-dentin regeneration.This study proposes a strategy to mimic the dental pulp regeneration microenvironment using nucleus pulposus microspheres (NPM) loaded with conditioned medium (CM) for dental pulp stem cells (DPSCs). NPM exhibits good biocompatibility, and NPMloaded CM enhances the odontogenic differentiation and angiogenic potential of DPSCs. Furthermore, the DPSCs+NPM+CM complexes promoted the regeneration of pulp-like tissue in an in vivo semi-isotopic model. Mechanistically, key bioactive cues secreted in the CM mediated the multi-directional differentiation potential of DPSCs, thereby facilitating pulp tissue regeneration. In summary, the 3D pulp-specific microenvironment, facilitated by NPM and CM bioactive factors, enables the regeneration of the pulp-dentin complex, offering a novel strategy and experimental basis for pulp regeneration.
Keywords: conditioned medium, microspheres, dental pulp stem cells, odontogenic differentiation, Endodontic regeneration
Received: 12 May 2025; Accepted: 07 Jul 2025.
Copyright: © 2025 Gao, Wang, Zhang, Qu, Liu, 陈, Xu and Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jing Shen, Tianjin Stomatological Hospital,Tianjin,China, Tianjin, China
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