Abstract
Nuclear morphology is a defining cellular feature, differing based on cell type, tissue type, and species. In healthy cells, nuclear morphology is generally tightly regulated and maintained; however, dynamic changes in nuclear morphology are observed under certain conditions, for instance in early embryos and in some immune cells. Deviations in normal nuclear morphology are linked to numerous diseases, including most cancers and premature aging syndromes. Many regulators of nuclear morphology have been identified, encompassing both intranuclear, cytoplasmic, and extracellular factors. Of note, recent studies have converged on chromatin and chromatin-associated proteins as key determinants of nuclear morphology and dynamics. In this review we discuss how the chromatin landscape regulates nuclear morphology in both normal and diseased cellular states. Additionally, we highlight emerging technologies that promise to bridge critical gaps in our understanding of nuclear morphology, including new approaches to probe nuclear structure and the use of synthetic cells.
1 Introduction
Cells vary substantially in size and morphology, ranging from yeast cells, which have a diameter of 3 μm, to nerve cells in the neck of the giraffe, which can be 3 m in length. A fundamental question is how organelle size and shape are tuned to support the structure and function of such diverse cell types. The regulation of nuclear size and shape is one area of particular interest (; ; ; ).
In eukaryotic cells, the nucleus is comprised of a double-membrane nuclear envelope (NE) that encloses the DNA and is often continuous with the endoplasmic reticulum. Nucleoporins are the proteins that make up nuclear pore complexes (NPCs) which span the NE, traversing through the inner and outer nuclear membranes (; ). Selective transport of molecules occurs through these NPCs (; ). The nuclear lamina is a meshwork that lines the nucleoplasmic face of the inner nuclear membrane and is composed of intermediate filament lamin proteins and additional interacting proteins, providing mechanical support and structural organization (Figure 1).
FIGURE 1
The maintenance of organelle shape and size is likely critical for cellular function. Under physiological conditions, nuclear sizes typically scale with cell sizeto maintain a constant nuclear-to-cytoplasmic ratio (; ; ; Neumann and Nurse, 2007; ; ; Vukovic et al., 2016; ; Sengupta et al., 2025). With respect to shape, nuclei are generally roughly spherical, although there are variations based on cell type, species, and differentiation status. Important questions in cell biology relate to the mechanisms responsible for the regulation of nuclear morphology.
Human granulocytes exhibit multi-lobed nuclei, connected by short channels of nucleoplasm (Skinner and Johnson, 2017). Spindly-shaped or fusiform nuclei are commonly seen in human fibrocytes and syncytial endosperm of flowering plants like Arabidopsis thaliana (Skinner and Johnson, 2017). Pathological conditions, such as cancers and laminopathies, are associated with significant alterations in nuclear morphology. Mutations in lamin A are known to cause Hutchinson-Gilford progeria syndrome, which is characterized by dysmorphic nuclei (Scaffidi and Misteli, 2006). Enlarged nuclei are observed in various forms of cancer. Furthermore, lobulated nuclei are characteristic of adenocarcinomas, and nuclei with grooves and clefts are seen in thyroid cancers (Zink et al., 2004). These anomalies in nuclear morphologies are often used by clinicians for diagnostic purposes. Nuclear inclusions and abnormal nuclei are also associated with neurodegenerative disease (Woulfe, 2008). Importantly, biological processes, including gene expression and cell migration, can be influenced by nuclear morphology. For example, preventing dynamic changes in nuclear morphology impedes the transition of cells to S phase (). Cells with altered nuclear elasticity due to changes in lamin A/C levels exhibit altered migration capabilities ().
In eukaryotes, genomic DNA is wrapped around histone octamers, comprising two copies each of the core histones H2A, H2B, H3, and H4, along with the linker histone H1. This facilitates the formation of highly condensed chromatin which, along with RNAs and other proteins, enables the packaging of DNA within the nucleus (). Chromatin structure can be broadly divided into two types: heterochromatin which is more condensed and inhibitory for DNA metabolic processes and euchromatin which is more open and accessible to DNA-binding factors. The dynamism between euchromatin and heterochromatin is regulated by ATP-dependent chromatin remodelers, post-translational histone modifications like methylation, acetylation, and phosphorylation, and direct DNA methylation (Suganuma and Workman, 2011; Nodelman and Bowman, 2021; ) (Figure 2). Of note, aberrations in these processes are the etiology of several diseases and disorders. Altered promoter methylation leads to aberrant gene expression in neurodevelopmental disorders like Parkinson’s, Alzheimer’s, and Huntington’s disease (). Gain and loss of function mutations in histone modifiers are observed in various forms of cancer, such as lung squamous cell carcinoma (; Papillon-Cavanagh et al., 2017; ), clear cell renal carcinoma (), myeloid neoplasms, breast and prostate cancer (Varambally et al., 2002), and others (). Chromatin is the major occupant of the nucleus and, as such, it is perhaps not surprising that the chromatin landscape can modulate nuclear morphology. In this review, we first provide a broad overview of known regulators of nuclear morphology and then focus on more recent studies that highlight the roles of chromatin structure and factors in regulating nuclear morphology and, in turn, cellular and nuclear function.
FIGURE 2
2 Regulators of nuclear morphology
An important determinant of nuclear morphology is the nuclear lamina (Figure 1). The nuclear lamina contributes to the ability of the nucleus to respond to mechanical forces and to withstand intracellular and extracellular forces to avoid catastrophe (Swift et al., 2013; Swift and Discher, 2014; Morival et al., 2025). Lamin A/C restricts nuclear deformation while facilitating movement through narrow channels with smooth surfaces. Experiments with fibroblasts cultured on arrays of fibronectin-coated micropost barriers that mimic collagen fiber bundles showed that lamin A/C facilitates nuclear passage between slender obstacles. Nuclei containing lamin A/C preserve their oval shape despite local indentations. Conversely, nuclei deficient in lamin A/C experience severe distortion and become entangled around obstacles, impeding movement. This supports a model where the nucleus deforms like a droplet, with lamin A/C providing surface tension that allows for local invaginations that enable forward movement while maintaining overall shape ().
Nuclei formed in Xenopus laevis egg extracts in the presence of dynamic F-actin exhibit a bilobed shape, with distinct membrane compositions in each lobe and F-actin concentrated at the inner nuclear envelope (Figure 1). Adding lamin A, not present in Xenopus eggs, results in more spherical nuclei. This indicates that a balance of forces exerted by nuclear F-actin and lamin A influences nuclear shape. Nuclear F-actin filaments, nucleated by formins, are thought to exert outward forces that alter nuclear morphology unless counterbalanced by lamin A (). Microtubules can also influence nuclear morphology, for instance short term proteasome inhibition causes microtubule-mediated NE deformation independently of nuclear import (Sengupta et al., 2025). Nuclear lamin concentration can influence nuclear growth and size, with low and high levels leading to increased and decreased nuclear size, respectively, regardless of the type of lamin expressed (). The phosphorylation of lamin B3 by protein kinase C plays a role in controlling nuclear size during early X. laevis development and in mammalian cells, indicating that this mechanism of nuclear size regulation is conserved (; ).
Nucleocytoplasmic transport is crucial for nuclear size regulation, as increased nuclear influx and inhibited efflux are associated with nuclear volume expansion and blebbing. In fission yeast, excessive accumulation of mRNA and protein within the nucleus results in enlarged nuclear size (). Factors that regulate nuclear import, such as Importin alpha and Nuclear Transport Factor 2 (NTF2), play a more significant role in determining nuclear size than the amount of DNA (Figure 1), perhaps by regulating the amount of nuclear lamins that are imported (). Radial growth phase primary melanoma cells exhibit larger nuclei than normal melanocytes, in particular when NTF2 levels are reduced. In patient-derived melanoma cells, increasing NTF2 expression leads to reduced nuclear size, decreased cell motility and proliferation, and enhanced apoptosis, implicating NTF2 as a melanoma tumor suppressor (Vukovic et al., 2021).
The nucleoporin ELYS plays a vital role in the assembly of the NPC following mitosis. In mammalian cells, a reduction in ELYS levels results in fewer NPCs, impaired nuclear import, diminished localization of nuclear lamin B2, and smaller nuclei. Some of these effects can be rescued by enhancing nuclear import through the overexpression of Importin alpha. Conversely, ELYS overexpression leads to a higher density of NPCs, increased import of nuclear lamin B2, and larger nuclei (). In other work, mutations that lead to the clustering and/or misplacement of NPCs result in changes to the shape of the nucleus (; Tamura and Hara-Nishimura, 2011; ). While these experiments show a correlation between NPC number/distribution and nuclear size, other studies suggest that NPC assembly and nuclear expansion are independently regulated (Ryan et al., 2003; ; Titus et al., 2010; ).
3 Chromatin and epigenetics as regulators of nuclear morphology
In the following sections, we discuss how chromatin structure and epigenetic determinants affect nuclear morphology and, if known, cell and organismal function.
3.1 Epigenetic factors, histones, and nuclear lamins
Epigenetic regulators affect nuclear dimensions and are linked to abnormal nuclear shape (; ; Schreiner et al., 2015; Stephens et al., 2017; Senigagliesi et al., 2019; Stephens et al., 2019a). For instance, overexpression of the histone acetyltransferase BRD4 leads to enlarged nuclei in HeLa cells (), and various chromatin components, including core histones, impact nuclear shape in MCF10A cells (Tamashunas et al., 2020). Depletion of the linker histone H1.0 was found to alter nuclear shape as measured using the elliptical Fourier coefficient ratio (Tamashunas et al., 2020). In hTERT-immortalized fibroblasts, expression of disease-relevant histone H3.3 mutations (e.g., K9M, K27M, K36M) caused nuclear shape abnormalities, including reduced nuclear size, decreased nuclear circularity, and a general increase in nuclear morphology variability across cells. These observed effects were not due to changes in cell number, toxicity, or lamin A localization (Schibler et al., 2023).
Interactions between chromatin and lamins further shape nuclear architecture (; Stephens et al., 2019a). Studies of nuclear mechanics showed that chromatin mediates responses to minor deformations whereas lamin A/C responds to greater forces (Stephens et al., 2017). Modifying the levels of euchromatin and heterochromatin influences nuclear structure and stiffness (Stephens et al., 2018; Stephens et al., 2019a), with increased heterochromatin reducing nuclear blebbing in compromised nuclei (Stephens et al., 2019a; Stephens et al., 2019b). The absence of the acetyltransferase MOF or its binding partners alters nuclear mechanics, associated with reduced lamin acetylation and epigenetic changes (). These observations highlight that nuclear size and shape are the result of intricate interactions between chromatin and nuclear structural proteins.
3.2 Lamin-independent effects
Chromatin is critical for the ability of the nucleus to withstand and respond to mechanical force (Reddy et al., 2008; Stephens et al., 2019a). Histone modifications are known to influence chromatin structure and nuclear morphology (Figure 2). Treating mammalian cells with histone deacetylase inhibitors to enhance euchromatin softens the nucleus, increasing blebbing (Stephens et al., 2018; ). Conversely, histone demethylase inhibitors that increase the amount of heterochromatin stiffen the nucleus, reducing blebbing (Stephens et al., 2018). In both scenarios, nuclear morphological changes occur independently of lamin alterations.
Although lamin disruptions are typically associated with nuclear blebbing (i.e., protrusions larger than 1 µm), changes in chromatin alone can lead to bleb formation without altered lamin levels. Mouse embryonic fibroblasts (MEFs) treated with a histone deacetylase inhibitor showed increased euchromatic H3K9ac and nuclear blebbing while lamin B1 and A/C levels remained unchanged (Stephens et al., 2017). Thus, chromatin decompaction alone can trigger nuclear blebbing without lamin depletion (Stephens et al., 2018). Contrary to studies linking nuclear blebbing with lamin B absence (Shimi et al., 2008), blebs induced with valproic acid (VPA) retained lamin B1 and A/C in 50% of treated MEF cells and 30% of treated HT1080 cells. Similar lamin B1 retention was observed in nuclear blebs when cells were treated with trichostatin A or 3-Deazaneplanocin A (DZNep) (; Stephens et al., 2017; Stephens et al., 2018; Stephens et al., 2019b; ), suggesting nuclear blebbing can occur as a result of chromatin-mediated reductions in nuclear rigidity without nuclear lamina disruptions (Stephens et al., 2018).
3.3 Chromatin structure and DNA amount
An emerging viewpoint is that mechanical properties of the nucleus can influence nuclear morphology (; Stephens et al., 2019b). Alterations in chromatin modifications and spatial arrangement are linked to changes in nuclear shape (Stephens et al., 2018; Stephens et al., 2019a; ; ). Elevating heterochromatin levels with histone demethylase inhibitors leads to increased chromatin stiffness that can rectify abnormal nuclear shapes (Stephens et al., 2018). As the nucleus expands, condensing chromatin takes up a smaller fraction of the nuclear space and increasing nuclear histone levels through the histone chaperone Npm2 further encourages nuclear enlargement (). Conversely, supplementing Xenopus egg extract with the histone methyltransferase Set9 () or DNA methyltransferase inhibitor Zebularine (Yoo et al., 2004) increased the proportion of nuclear space filled by chromatin, with Set9 elevating H3K4me1 and Zebularine reducing 5-methylcytosine levels. These nuclei showed diminished growth and smaller final sizes, further highlighting how chromatin structure can affect nuclear size ().
Nuclei formed in Xenopus egg extract almost completely stopped growing when treated with DNA degrading benzonase, regardless of the nuclear size when treatment was initiated (). Nuclear F-actin plays a role in the expansion of the nucleus (; ) but benzonase-treated nuclei formed in extracts with intact actin still failed to expand. These findings suggest that DNA is crucial for nuclear growth, even in the presence of F-actin (). Massive increases in DNA content can affect nuclear size as nuclei assembled in X. laevis egg extract using axolotl sperm chromatin, which contains 20-fold more DNA than Xenopus, exhibited a doubling in nuclear cross-sectional area (). This indicates that nuclear size can be responsive to DNA quantity (; ). When transcriptionally inert Xenopus egg extracts were treated with VPA to increase H3K9ac or DZNep to decrease H3K27me3, nuclei were smaller without any change in nuclear import rate. Conversely, increasing H3K27me3 with the histone demethylase inhibitor Methylstat or increasing 5-methylcytosine levels with the DNA methylator NDMA led to significant nuclear enlargement. This study showed that even with similar nuclear import rates, altered chromatin structure can reduce or increase nuclear growth in a transcription-independent manner ().
3.4 Cellular mechanics and mechanosensation
The intermediate filament protein Keratin 17 can be found within the nucleus, interacting with proteins that play a role in organizing chromatin. Keratin 17 knockout in tumor-derived HeLa and A431 cell lines leads to a decrease in histone methylation and acetylation with a concomitant increase in nuclear size (). Fibroblasts, the predominant cells within connective tissues, are subjected to substantial compressive forces from the surrounding extracellular matrix and fluid during activities like walking, sitting, and sleeping. Applying a static compressive force to cultured mouse fibroblasts increases nuclear levels of histone deacetylase 3, which promotes heterochromatin formation. Upon removal of the compressive force, the cells revert to their original chromatin condensation state (). Whether nuclear morphology is affected under these conditions is an interesting area for future study.
Cell stretching activates mechanosensitive channels, triggering transient calcium influx () and increased chromatin compaction (; ; ). Mechano-transduction mediated by these channels can protect against abnormal nuclear morphology. For example, in VPA-treated MEFs that exhibit chromatin decompaction, increasing the extracellular concentration of magnesium chloride induced heterochromatin formation through histone methyltransferases, leading to a reduction in nuclear blebbing. These cells also showed increased short-extension nuclear spring constants with no change in long-extension stiffness (Stephens et al., 2019b). Interestingly, MEF cells co-treated with VPA and the transcription inhibitor alpha-amanitin also exhibited reduced nuclear blebbing (). Treating SKOV3 cells with trichostatin A to increase histone acetylation led to chromatin decompaction and a reduction in extracellular vesicle production upon cellular compression (Toth et al., 2004).
3.5 DNA damage and nuclear blebs
One of the hallmarks of many human diseases is DNA damage. MEFs and HT1080 cells treated with the DNA damaging agents cisplatin and bleomycin exhibited increased nuclear blebbing independently of passage through mitosis. Furthermore, DNA damage-induced nuclear blebs proceeded to rupture at a >90% frequency (Stephens et al., 2019b; ). Disrupting DNA damage response pathways, including p53, Rb, and BRCA2, can also increase the frequency of nuclear rupture (Yang et al., 2017; ). Micromanipulation force measurements of isolated vimentin-null MEF nuclei revealed that DNA damage significantly reduces chromatin-based nuclear stiffness (Stephens et al., 2017; ; ). Heterochromatin was reduced in response to DNA damage through activation of Ataxia-telangiectasia Mutated kinase (ATM), leading to chromatin softening, reduced nuclear rigidity, and increased nuclear blebbing and rupture (Ziv et al., 2006; ; ). Inhibiting ATM during DNA damage treatment rescued heterochromatin levels and restored nuclear mechanics, shape, and integrity (). Defects in nuclear morphology and integrity caused by DNA damage exacerbate a cycle of nuclear dysfunction in both confined and unconfined cells (; Xia et al., 2018; Stephens et al., 2019b; Pho et al., 2024). Furthermore, inhibition of actin contractions can rescue DNA damage-induced changes in nuclear morphology (Pho et al., 2024).
Nuclear blebs often exhibit a unique chromatin signature. In MEFs, HT1080 fibrosarcoma, and PC3 prostate cancer cell lines it was recently discovered that nuclear blebs tend to exhibit reduced DNA density but that bleb formation does not correlate with lamin B1 levels (). Interestingly, increased DNA damage in such blebbed nuclei was independent of rupture (). Disrupted nuclear morphology, including blebs, is associated with multiple disorders, and chromatin structure within nuclear blebs is often altered (Pujadas Liwag et al., 2025). There is direct evidence that the chromatin methylation status affects nuclear morphology. Loss of H3K9me3 increases nuclear blebbing and rupture due to decreased nuclear rigidity, while loss of H3K9me2 decreases nuclear blebbing and rupture accompanied by increased nuclear rigidity and more compact chromocenters (). Biological processes like transcription can also regulate nuclear morphology, for instance chemical inhibition of transcription can suppress nuclear bleb formation and rupture (). Taken together, DNA damage and chromatin status are important determinants of nuclear blebbing and rupture.
3.6 Cell migration, differentiation, and senescence
Alterations in chromatin structure and nuclear shape contribute to cell migration. In tenocytes, mechanical stretch caused reduced levels of H3K27me3 and increased levels of H3K9ac and H3K27ac, which in turn led to changes in nuclear morphology independently of lamin A/C, promoting tenocyte migration (Xu et al., 2023). Additionally, inhibiting histone methyltransferases with small molecule inhibitors affected nuclear morphology and integrity, diminished wound closure efficiency, and impeded cellular migration (). In chicken primordial germ cells, disruption of the transcription factor Oct4 led to a reduction in H3K27ac modifications on active chromatin regions and altered cell migration ().
The human immune system depends on a variety of cell types to establish and maintain its surveillance capabilities. Nuclear lobulation, a characteristic of many human immune cells, is a result of increased NE deformability and increased chromatin-NE interactions (Olins et al., 2001). Neutrophils adopt malleable polymorphonuclear structures by halting chromatin loop extrusion, thus enabling them to migrate through narrow interstitial spaces (Patta et al., 2024). Short range genome reorganization favors neutrophil migration such that the nucleus can alter its shape without causing chromatin damage ().
Cell differentiation is often associated with changes in nuclear stiffness and morphology. For example, nuclei in embryonic stem cells are deformable and become six times stiffer in their terminal stages of differentiation (Pajerowski et al., 2007). Furthermore, increases in cell size have been shown to contribute to cellular senescence through genome dilution (Neurohr et al., 2019; ; ). Given that nuclear size typically scales with cell size, an interesting area for future study is whether increased nuclear size might also contribute to the onset of senescence.
3.7 Epigenetics and disease
Epigenetic modifications profoundly affect gene expression, and aberrant modifications have been linked to various diseases like cancer, neurological disorders, cardiovascular diseases, and more recently COVID-19 syndrome (Table 1) (Vodovotz et al., 2020). How epigenetic changes give rise to disease is a broad topic that has been extensively reviewed (; ; ; ; Tollefsbol, 2024). Here we will only touch on a few illustrative examples. In cancers, extensive DNA methylation and histone deacetylation occur within tumor suppressor genes, which results in their silencing and ultimately exacerbates disease. Aberrant DNA methylation and histone demethylation have been shown to promote the epithelial-to-mesenchymal transition in breast cancer cells, causing the cancer to proliferate and metastasize (Serrano-Gomez et al., 2016). Breast tumors defective for ARID1A, a component of the chromatin remodeling complex involved in nucleosome sliding, exhibit altered recruitment of histone deacetylase 2 and uncontrolled cell growth (Xu et al., 2020). Aberrations in epigenetic mechanisms are not limited to breast cancer. In glioblastoma multiforme, a rare and incurable adult brain tumor, hypermethylation of DNA is found in genes that regulate the WNT, Frizzled, and Ras pathways (; ; ), contributing to cell proliferation. Mutations in ATP-dependent chromatin modifiers, including SMARCB1, BRG1, and BRM, have been identified in primary tumors and tumor-derived cell lines, including rhabdoid tumors, chronic myeloid leukemia, lung cancer, and prostate cancer (Salvatore and Vandenplas, 2003; ).
TABLE 1
| Disease | Epigenetic changes | References |
|---|---|---|
| COVID-19 | DNA methylation, histone acetylation | Pinto et al. (2020),Vodovotz et al. (2020), |
| Breast cancer | DNA methylation, histone acetylation, histone methylation | Serrano-Gomez et al. (2016),Xu et al. (2020) |
| Glioblastoma multiforme | DNA hypermethylation | |
| Type II diabetes | DNA hypermethylation | Yang et al. (2012), |
| Schimke immune-osseous dysplasia | Mutated chromatin remodeler SMARCAL1 | |
| Pediatric glioblastoma multiforme, pediatric adreno cortical tumor | Mutation in chromatin remodeler ATRX1 | Schwartzentruber et al. (2012), |
| Alzheimer’s disease | DNA methylation | Santana et al. (2023) |
An outline of diseases that are associated with epigenetic alterations.
Altered epigenetics are associated with other diseases in addition to cancer. Hutchinson-Gilford progeria syndrome is characterized by abnormal nuclear shape, and increasing heterochromatin can restore normal nuclear morphology in cell lines that model the disease and in patient-derived cells (Scaffidi and Misteli, 2005). Altered DNA methylation patterns are observed in pancreatic islets and adipose and muscle tissues of type 2 diabetic mice and patients (; ). In pancreatic islets, hypermethylated DNA can be detected in genes that regulate insulin secretion (Yang et al., 2012; ). In Alzheimer’s, altered DNA methylation is observed in disease-associated genes like amyloid precursor protein, Apolipoprotein E, and Ankyrin 1 (; Santana et al., 2023). Mutations in the ATRX chromatin remodeling complex cause several X-linked syndromes that manifest with facial dysmorphism, urogenital defects, and α-thalassemia (). Mutations in the chromatin remodeler SMARCAL1 are a known cause of Schimke immuno-osseous dysplasia, an autosomal recessive disorder associated with T-cell immunodeficiency, spondyloepiphyseal dysplasia, renal failure, and other symptoms (). Thus, normal cellular physiology depends on precise regulation of the epigenetic state of chromatin and proper function of chromatin-associated molecules including histones and chromatin modifiers. Open questions relate to whether epigenetics influence disease phenotypes through altered nuclear morphology.
4 Emerging techniques and models in the field of nuclear morphology
Over the past 150 years, there have been significant advancements since Sir Lionel Beale first observed alterations in typical nuclear structures in various diseases, including cancer. To this day, the Pap smear, introduced by George Papanicolaou, remains a diagnostic tool for detecting abnormal nuclear structures in cervical tissue samples (). Microscopy serves as an essential means of examining the nuclear condition of cells, offering crucial insights into nuclear shape, nuclear mechanics, protein distribution, and genome arrangement (). Researchers are beginning to elucidate the connections between mechanics and morphology by integrating atomic force microscopy with side-view light sheet microscopy. Offering high spatiotemporal resolution, this approach has been used to observe cells during compression, leading to empirical models for nuclear deformation applicable to atypical nuclear shapes (; ). RD-SPRITE explores the interactions between chromatin and RNA (Quinodoz et al., 2021). Spatial multi-omics, which combines FISH and immunofluorescence techniques, have been used to chart nuclear architecture, and in situ genome sequencing facilitates the simultaneous visualization and sequencing of the genome at the nucleotide level (Palihati and Saitoh, 2024). Microfluidic devices constructed from polydimethylsiloxane can be used to replicate physiological conditions for the study of intracellular mechanics and dynamics, mimicking conditions relevant to cancer cell invasion and immune cell recruitment ().
The intricate nature of cells and their organelles poses challenges in studying fundamental molecular processes within living organisms. One approach to addressing this issue is bottom-up synthetic biology, which involves creating life-like systems from molecular components (; ; ). This multidisciplinary area incorporates chemistry, biology, and engineering (Sismour and Benner, 2005). One of the challenges in bottom-up synthetic biology is to develop systems that mimic life and sustain physiochemical balance using a minimal set of components (Smigiel et al., 2019). A model system commonly used to study organelle size, morphology, and function is cell-free Xenopus egg extract. Nuclei assembled in Xenopus extract exhibit the typical structures and activities of nuclei found in living cells (; Mishra and Levy, 2022b). The open biochemical nature of the system allows for the addition of recombinant proteins and removal of endogenous proteins by immunodepletion, as well as exogenous addition of small molecule inhibitors or activators without pleiotropic effects on transcription, translation, and cell cycle progression (Sengupta et al., 2025). Furthermore, fluorescently labeled proteins allow for live time-lapse microscopy of organelle dynamics (). Lastly, high-throughput imaging for siRNA screening represents an effective approach to identify regulators of nuclear architecture in an unbiased manner (Schibler et al., 2023). Interestingly, this latter study identified a number of nuclear morphology effectors in two different cell types but there was very little overlap in the hits, highlighting unresolved questions about the complexity of nuclear morphology control.
5 Conclusion
How nuclear morphology is regulated is a fundamental question of cell biology which remains to be understood completely. This regulation is critical as numerous diseases present with abnormal nuclear phenotypes that are often used by clinicians for diagnostic purposes. Several factors have been identified as contributors to this regulation; however important knowledge gaps still exist. In this review, we have discussed some of the known regulators of nuclear morphology, with a particular focus on how chromatin influences the regulation of nuclear structure and function. Aberrations in epigenetic modifications are the underlying cause for numerous diseases (). At the same time, changes in the epigenetic landscape also impact nuclear morphology and function. To what extent aberrant nuclear morphology, altered epigenetic status, and disease phenotype are linked is a critical open question. Simplified model systems like Xenopus egg extract and novel approaches like hydrogel chambers are being increasingly used to mimic physiological cellular conditions and advance our knowledge of fundamental processes like the regulation of nuclear morphology. At the same time, advanced microscopy techniques have improved our ability to visualize component molecules and structures relevant to epigenetics and nuclear morphology. The next decade promises to answer key questions at the intersection of chromatin biology, nuclear morphology, and disease.
Statements
Author contributions
SS: Writing – original draft, Conceptualization, Writing – review and editing. HP: Conceptualization, Writing – original draft, Writing – review and editing. DL: Supervision, Writing – review and editing, Conceptualization, Funding acquisition.
Funding
The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by NIH grants R35GM134885 and P20GM103432.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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The author(s) declare that no Generative AI was used in the creation of this manuscript.
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Summary
Keywords
nuclear size, nuclear shape, chromatin structure, chromatin modifications, cancer, epigenetics, Xenopus egg extract, microscopy
Citation
Sengupta S, Prabha H and Levy DL (2025) How the chromatin landscape influences nuclear morphology. Front. Cell Dev. Biol. 13:1634252. doi: 10.3389/fcell.2025.1634252
Received
23 May 2025
Accepted
24 June 2025
Published
03 July 2025
Volume
13 - 2025
Edited by
Coral Zhou, University of Kansas, United States
Reviewed by
Abigail Buchwalter, University of California San Francisco, United States
Helena Cantwell, University of California, Berkeley, United States
Andrew Stephens, University of Massachusetts Amherst, United States
Updates
Copyright
© 2025 Sengupta, Prabha and Levy.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sourabh Sengupta, sourabh.sengupta@utsouthwestern.edu; Daniel L. Levy, dlevy1@uwyo.edu
† These authors have contributed equally to this work and share first authorship
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