REVIEW article
Front. Cell Dev. Biol.
Sec. Stem Cell Research
Single-cell cloning and its approaches
Provisionally accepted
- 1Division of Medicine, University College London, London, United Kingdom
- 2Faculty of Science, Cairo University, Giza, Egypt
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Single-cell cloning (SCC) is a technique for the production of a pure clone from a single parental cell (1). SCC has become a fundamental process in biological research. It has many crucial applications, ranging from the production of therapeutic proteins to stem cell-based therapies (2,3). However, a main obstacle with SCC has been the difficulty in establishing and scaling single-cell derived clones (4). Various methods and tools have been developed to facilitate SCC. This review provides an overview of the most common techniques for SCC. The selection among these techniques is contingent upon several factors, including available resources, cell proliferation capacity, number of needed clones, and expertise.
Keywords: single cells, Cell cloning, Clonal cultures, single-cell sorting, Clone isolation and expansion
Received: 01 Aug 2025; Accepted: 21 Nov 2025.
Copyright: © 2025 Ammar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Amany Ammar, amany.ammar.19@ucl.ac.uk
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.