CASE REPORT article

Front. Endocrinol., 10 July 2017

Sec. Pediatric Endocrinology

Volume 8 - 2017 | https://doi.org/10.3389/fendo.2017.00157

A Case with Spondyloenchondrodysplasia Treated with Growth Hormone

  • TU

    Takanori Utsumi 1*

  • SO

    Satoshi Okada 2

  • KI

    Kazushi Izawa 3

  • YH

    Yoshitaka Honda 3

  • GN

    Gen Nishimura 4

  • RN

    Ryuta Nishikomori 3

  • RO

    Rika Okano 1

  • MK

    Masao Kobayashi 2

  • 1. Department of Pediatrics, Onomichi General Hospital, Hiroshima, Japan

  • 2. Department of Pediatrics, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan

  • 3. Department of Pediatrics, Kyoto University Graduate School of Medicine, Kyoto, Japan

  • 4. Department of Pediatric Imaging, Tokyo Metropolitan Children’s Medical Center, Tokyo, Japan

Abstract

Spondyloenchondrodysplasia (SPENCD) is an autosomal recessive skeletal dysplasia caused by loss of function mutations in acid phosphatase 5, tartrate resistant (ACP5). Hypomorphic ACP5 mutations impair endochondral bone growth and create an interferon (INF) signature, which lead to distinctive spondylar and metaphyseal dysplasias, and extraskeletal morbidity, such as neurological involvement and immune dysregulation, respectively. We report an affected boy with novel ACP5 mutations, a splice-site mutation (736-2 A>C) and a nonsense mutation (R176X). He presented with postnatal short stature, which led to a diagnosis of partial growth hormone (GH) deficiency at 3 years of age. GH therapy was beneficial in accelerating his growth velocity. At 6 years of age, however, metaphyseal abnormalities of the knee attracted medical attention, and subsequent assessment ascertained the typical skeletal phenotype of SPENCD, brain calcifications, and an INF signature. This anecdotal experience indicates the potential efficacy of GH for growth failure in SPENCD.

Introduction

Spondyloenchondrodysplasia (SPENCD, OMIM #607944) is a rare autosomal recessive skeletal dysplasia characterized by vertebral dysplasia and enchondroma-like radiolucent metaphyseal lesions of the long bones (1). Affected individuals manifest with short-trunked short stature. In addition, they show a variety of extraskeletal abnormalities, including neurological symptoms (intracranial calcification, developmental delay, spasticity, clumsy movements), and immune dysregulation (autoimmune diseases, immunodeficiency) (2–10). Recently, two groups reported biallelic hypomorphic mutations in the Acid Phosphatase 5, Tartrate Resistant (ACP5) gene as a genetic cause of SPENCD (11, 12). ACP5 encodes tartrate-resistant acid phosphatase (TRAP). TRAP deficiency increases levels of phosphorylated osteopontin, which causes dysregulated endochondral ossification and other manifestations, and also an interferon (INF) signature (increased expression of type I INF regulated genes) leading to extraskeletal complications (11–13). Very little is documented concerning medical intervention for short stature and INF signature in SPENCD. Some SPENCD reports have mentioned attempts of growth hormone (GH) therapy, but the effect of GH has not been fully detailed (4, 10).

We report here an affected Japanese boy with novel compound heterozygous ACP5 mutations, who had the typical skeletal phenotype of SPENCD, brain calcifications, absence of TRAP activity, and laboratory findings of an INF signature, but no immunodeficiency or autoimmune disorders. He had developed short stature in early childhood, leading to a diagnosis of partial GH deficiency (GHD) and introduction of GH therapy prior to the definitive diagnosis of SPENCD. Interestingly, his growth velocity was significantly accelerated in response to GH treatment.

Case Report

The patient is a 6-year-old Japanese boy who was born to non-consanguineous healthy parents. The patient’s mid-parental target height was 180 cm (final height of his father and mother was 177 and 170 cm, respectively). His older brother was healthy (Figure 1A). He was born at term after an uncomplicated pregnancy and delivery. Birth weight was 3,055 g (−0.64 SD), length 51.5 cm (+1.06 SD), and head circumference 34.0 cm (+0.40 SD). At 19 months, he was referred to us because of his inability to walk unaided. At that time brain MRI yielded a normal finding. He entered a rehabilitation program that improved his ambulation. His linear growth started to slow down from 5 months of age. At 38 months of age his height was 84.5 cm (−2.89 SD) (Figure 1B). Thyroid function was normal (TSH 4.520 μU/mL, FT4 1.06 ng/dL). The level of baseline insulin-like growth factor I (IGF-I) was low (18 ng/mL). These clinical manifestations and results of a GH stimulation test led to the diagnosis of partial GHD (peak GH response to arginine was 4.60 ng/mL and to clonidine was 2.41 ng/mL; the cut-off point was <6 ng/mL to define partial GHD and <3 ng/mL to define severe GHD) (Table S1 in Supplementary Material). GH therapy (0.175 mg/kg/week) was introduced, and it significantly accelerated his growth velocity (Figure 1B).

Figure 1

At 6 years of age, he presented with painful knee joints. Height was 109.5 cm (−1.04 SD), and arm span was 111 cm. Facial features and body proportion were normal (Figure 2A). Development (language and cognitive skills, and fine and gross motor skills) was appropriate for his age, other than some clumsiness in motion. A radiograph of the knee showed mild metaphyseal dysplasia of the knee (shaved contour and marginal spur in the distal femoral metaphyses and to a lesser extent in the proximal tibial metaphyses, and mild cupping proximal fibular metaphyses) (Figure 2C). A subsequent skeletal survey revealed generalized metaphyseal dysplasia involving the hip and wrist as well as the knee (Figures 2D,E). The spine showed generalized platyspondyly with irregular endplates. The posterior vertebral bodies were irregularly ossified (Figure 2B). These radiographic findings raised a suspicion of SPENCD. Laboratory data showed normal levels of serum calcium, phosphate, alkaline phosphatase, intact PTH, IGF-I, and thyroid function (Table S1 in Supplementary Material). No immunological abnormality was found, including normal levels of complements and no detectable autoantibodies. TRAP activity was undetectable (TRAP-5b < 0.1 U/L). Bone mineral density on dual-energy x-ray absorptiometry was normal (L1–L4: 0.942 g/cm2). Brain CT of the patient showed bilateral calcifications of the basal ganglia (Figure 2F).

Figure 2

Materials and Methods

Genetic Analysis

The genetic study of the patient was performed using a candidate gene approach with Sanger sequencing of the ACP5 gene. A familial study was not performed because consent was not given.

INF Analysis

The expression of INF-stimulated genes in the patient’s whole blood cells were measured by qPCR as described previously (14). Whole blood was collected into PAXgene tubes (PreAnalytix, Hombrechtikon, Swizerland) and frozen at −20°C until extraction using the manufacturer’s protocol. Total RNA was extracted with a PAXgene RNA Blood kit (PreAnalytix) and reverse-transcribed to cDNA using an Omniscript RT kit (Qiagen, Hilden, Germany). Real time quantitative PCR was performed in triplicate using a 7900HT Fast Real-Time PCR system (Applied Biosystems, Waltham, MA, USA) and a kit for each INF-stimulated gene (IFI27, Hs01086370_m1; IFIT1, Hs00356631_g1; RSAD2, Hs01057264_m1; SIGLEC1, Hs00988063_m1; ISG15, Hs00192713_m1; IFI44L, Hs00199115_m1; BACT, Hs01060665_g1) (Life Technologies, Carlsbad, CA, USA). The expression levels were normalized to those of β-actin. Results are shown relative to a single calibrator (control 1).

Results

Identification of ACP5 Mutations

The sequence analysis revealed novel ACP5 mutations, a splice-site mutation (c.736-2 A>C) and a nonsense mutation (c.526 C>T; p.R176X) (Figures 3A,B). Neither 736-2 A>C nor R176X were found in the NCBI, Ensembl, dbSNP, or ExAc databases.

Figure 3

Detection of INF Signature

We identified marked upregulation of IFI27, IFIT1, IFI44L, ISG15, RSAD2, and SIGLEC1 in the patient compared with healthy controls (n = 8) (Figure 3C).

Discussion

We report a boy with a typical SPENCD skeletal phenotype, who presented with postnatal short stature and brain calcifications. He has not developed immune dysregulation despite laboratory evidence of a type I INF signature. An IFN signature in SPENCD plays a major role in neurological and immune involvement. We summarized the clinical manifestations of 48 patients with SPENCD by reviewing previous reports, as presented in Table 1 (1–9, 15–22). Height ranged from −1.5 to −7.5 SD (only cases with SD notation were included). The common features that first prompt the seeking of medical attention include musculoskeletal symptoms (60.4%), such as short stature, short limbs, kyphoscoliosis, and bowlegs, followed by neurological symptoms (27.1%) and immune dysregulation (18.8%). On the other hand, Briggs et al. (10) reviewed 26 patients, including 12 previously reported patients, and identified that up to 50% initially presented with immune dysregulation, followed by musculoskeletal symptoms (46.2%) and neurological symptoms (23.1%). This difference between our review and Briggs’ may represent the clinical diversity of SPENCD.

Table 1

AuthorPatient no.GenderAt initial presentation
Growth failureNeurological symptomsIntracranial calcificationImmune dysregulationReference
AgeClinical symptoms
Schorr et al.Pt. 1M8 yearsShort statureYesNoNDNo(1)
Pt. 2M5 yearsShort statureYesNoNDRecurrent infections

Gustavson et al.Pt. 3F15 monthsShort stature−7.5 SD at 16 yearsaNoNDND(15)
Pt. 4MBirthShort stature, short limbs, bowlegs−6.5 SD at 13 yearsaNoNDND

Sauvegrain et al.Pt. 5F16 yearsShort stature, short neckYesNoNDND(16)
Pt. 6M7 yearsShort stature, Dev. delayYesDev. delayNDND

Chagnon et al.Pt. 7M8 yearsSpastic quadriplegiaYesSpasticityNDND(17)

Menger et al.Pt. 8MBirthShort limbs−6 SD at 12 yearsDev. delayNDND(18)
Pt. 9MBirthShort limbs−6 SD at 9 yearsDev. delayNDND
Pt. 10M4 monthsCraniotabes, costochondral beading−1.5 SD at 12 yearsNoNDND
Pt. 11M6 yearsShort stature, spastic quadriplegia, kyphoscoliosis−2 SD at 6 yearsDev. delay and spasticityNDND

Frydman et al.Pt. 12M2.5 yearsShort stature−2.5 SD at 11 yearsNoNoNo(2)
Pt. 13F2 yearsShort stature−3.5 SD at 8 yearsNoNoND
Pt. 14M10 yearsNDNDNDNDND
Pt. 15M15 yearsShort stature, kyphosis, pectus carinatum, spastic quadriparesis−3 SD at 15 yearsSpasticityYesND
Pt. 16M2 yearsWalking difficulties, spastic quadriparesis−4.4 SD at 8 yearsDev. delay and spasticityYesND
Pt. 17M18 yearsLack of ejaculate−5.4 SD at 18 yearsNoYesND

Robinson et al.Pt. 18M11 yearsShort stature, joint pain<3rd percentile at 19 yearsNoNDND(19)
Pt. 19MNDND−5.5 SD at 86 yearsaNDNDND

Uhlmann et al.Pt. 20MBirthShort stature, clubfoot, diastasis recti, low-set ears, simian creases, short neck<3rd percentile at 5 yearsNoNDNo(20)
Pt. 21M11 monthsShort statureYesNoNDNo

Roifman and MelamedPt. 22F5 monthsRecurrent infections<3rd percentile at 18 yearsNoNDRecurrent infections, ITP, hypothyroidism, vitiligo(3)
Pt. 23M3 monthsRecurrent infectionsNDNoNoRecurrent infections, ITP, hypothyroidism
Pt. 24M3 yearsRecurrent infectionsYesNoNDRecurrent infections, ITP
Pt. 25MInfancyRecurrent infectionsAround 10th percentile at 10 yearsNoNDRecurrent infections, JRA, Crohn’s disease, hypothyroidism, vitiligo

Tuysuz et al.Pt. 26M10 yearsShort stature<3rd percentile at 10 yearsNoNoND(4)
Pt. 27F9 yearsShort stature<3rd–10th percentile at 21 yearsNoNoND
Pt. 28M7 yearsShort stature, Dev. delay<3rd percentile at 7 yearsDev. delayYesND

Bhargava et al.Pt. 29M7 yearsBack pain, kyphosis<5th percentile at 13 yearsNoNDNo(21)
Pt. 30FBirthShort stature<5th percentile at 42 yearsNDNDND

Renella et al.Pt. 31M3 yearsShort stature<3rd percentile at 3 yearsNoNoSLE(5)
Pt. 32F8 monthsLeg stiffness<3rd percentile at 3 yearsDev. delay and spasticityYesNo
Pt. 33F1 monthsShort statureYesNoNDNo
Pt. 34M1 monthsLeg stiffness<3rd percentile at 18 yearsDev. delay and spasticityYesAIHA, FUO, ITP
Pt. 35M21 monthsLeg stiffness, spastic paraparesisYesDev. delay and spasticityNDITP, AIHA
Pt. 36M10 yearsRecurrent thrombocytopenia, recurrent fever of unknown origin<3rd percentile at 17 yearsDev. delay and spasticityYesITP, FUO
Pt. 37MChildhoodShort statureYesSpasticityNDND
Pt. 38F11 yearsRheumatic fever with carditis, sydenham chorea<3rd percentile at 12 yearsNoNDRheumatic fever
Pt. 39M8 monthsShort stature<3rd percentile at 7 yearsDev. delay and spasticityNoInflammatory syndrome with hypogammaglobulinemia, AIHA

Kulkarni et al.Pt. 40M2 yearsRecurrent infections<5th percentile at 5 yearsNoNDRecurrent infections, compromised cellular immunity, SLE, ITP, JRA(6)

Navarro et al.Pt. 41F3 yearsFebrile seizures<5th percentile at 23 yearsDev. delayYesSjögren syndrome, polymyositis, hypothyroidism, pancreatitis, autoimmune multifocal neuropathy, scleroderma(7)
Pt. 42M2.5 yearsWalking difficulties, skin rash<5th percentile at 10 yearsSpasticityYesLeukocytoclastic vasculitis, SLE

Girschick et al.Pt. 43F6 monthsIncreased muscle tone of the lower limbsYesSpasticityNoAIHA, ITP, polyarthritis, hepatitis, nephritis, life-threatening hyperreactivity to viral infections as well as recurrent bacterial infections(8)

de Bruin et al.Pt. 44F13 yearsShort stature−5.5 SD at 13 yearsDev. delayNoNo(22)
Pt. 45M8 yearsShort stature−5.1 SD at 8 yearsDev. delayNoNo

Bilginer et al.Pt. 46M5 yearsShort stature<3rd percentile at 5 yearsNDYesSLE (lupus nephritis)(9)
Pt. 47F16 yearsShort stature, arthralgia, arthritis<3rd percentile at 16 yearsNDYesSLE (lupus nephritis), vitiligo
Pt. 48F4.5 yearsLeg stiffness, walking difficulties<3rd percentile at 16 yearsSpasticityYesSLE (lupus nephritis)

Literature review of SPENCD case reports.

M, male; F, female; ND, not described; Dev. delay, developmental delay; ITP, idiopathic thrombocytopenic purpura; JRA, juvenile rheumatoid arthritis; SLE, systemic lupus erythematosus; AIHA, autoimmune hemolytic anemia; FUO, fever of unknown origin.

aDescribed in Ref. (10).

In the present case, postnatal growth failure was ameliorated with GH therapy; height increased from −2.89 SD to −1.04 SD over 3 years. The patient’s IGF-I levels increased to within the normal range after commencing GH therapy (Figure S1 in Supplementary Material). In addition, GH therapy did not accelerate bone age; bone ages were 2.3 and 5.2 years at the chronological ages of 3 years 4 months and 6 years 11 months, respectively. These findings indicate the potential therapeutic benefit of GH to improve short stature in patients with SPENCD.

Two reports have been published describing three SPENCD patients who underwent GH therapy (4, 10). Briggs et al. (10) described two patients, including one patient with GHD who responded well to GH therapy. Tuysuz et al. (4) reported a 21-year-old woman with SPENCD who received GH therapy for 2 years from the age of 9 years such that she achieved a normal height (153 cm: 3–10 centile). It remains unclear whether a good response to GH therapy in these SPENCD patients is universal or not. Lack of TRAP leads to an increase in phosphorylated osteopontin, which is responsible for disordered endochondral ossification and reduced resorption of calcified cartilage matrix and primary spongiosa as a result of impaired adhesion, migration, and activation of osteoclasts (11, 13, 23, 24). Meanwhile, GH advances longitudinal bone growth directly by stimulating prechondrocyte differentiation, and indirectly by clonal expansion of differentiated chondrocytes through upregulation of IGF-I (25–29). Moreover, GH stimulates resorption of cartilage matrix and immature bone through both its direct and indirect actions on osteoclast formation and differentiation and through indirect activation of mature osteoclasts via osteoblasts. Upregulation of IGF-I associated with GH therapy also supports osteoclastic activities (30, 31). These effects of GH may efficiently counteract the negative effects on endochondral bone growth in SPENCD.

Growth hormone therapy is associated with adverse risks in some patients, such as femoral head necrosis, slipped capital femoral epiphysis, exostosis, and the progression of bone deformities, particularly spinal deformities, such as scoliosis. We have not observed any obvious adverse events associated with GH therapy in the present case up to this time. However, because only 3 years has passed since the patient commenced GH therapy, the patient will require regular follow-up to evaluate the potential long-term effects of GH therapy. In addition, there are some other limitations in our study. First, the patient has partial GHD as well as SPENCD, and therefore the potential benefit of GH therapy for short stature in patients with SPENCD might be overestimated. Second, the patient is 6 years old and is too young to evaluate the effect of GH therapy on his final height. Despite these limitations, however, the improvement in growth velocity found in the present case is encouraging and may suggest the potential therapeutic benefit of GH in patients with SPENCD.

Concluding Remarks

This case report described the treatment of a patient who presented progressive growth failure associated with SPENCD and partial GHD. After commencing GH therapy, the patient’s height SD score improved from −2.89 SD to −1.04 SD over 3 years without accelerating the bone age. Considering the improvement in growth velocity in the present case, it is reasonable to suggest that GH therapy is warranted in patients with SPENCD, at least for those who show evidence of a partial GHD. Further studies are recommended to evaluate the benefit of GH therapy in improving the height of patients with SPENCD, even in the absence of GHD.

Statements

Ethics statement

This study was approved by the Institutional Review Board of Kyoto University. We obtained written informed consent for genomic and interferon analysis of the patient from the parents in accordance with the Declaration of Helsinki. Also, the parents of the patient provided written informed consent for the publication of the patient’s identifiable information.

Author contributions

Patient workup: TU. Interpretation of radiographs: GN. Genetic and IFN signature analysis: KI, YH, and RN. Interpretation of data, drafting the manuscript or critical revision, final approval of the version to be published, and agreement to be accountable for all aspects of the work: TU, SO, KI, YH, GN, RN, RO, and MK.

Funding

This study was supported in part by the Practical Research Project for Rare/Intractable Diseases from the Japan Agency for Medical Research and Development, AMED.

Acknowledgments

We sincerely appreciate our patient and his family for their kind cooperation. We also thank Dr. Y. Sumen (Department of Orthopaedic Surgery, Onomichi General Hospital, Hiroshima, Japan) and Dr. T. Shimura (Department of Orthopaedic Surgery, Hiroshima Prefectural Rehabilitation Center, Hiroshima, Japan) for diagnostic advice.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

The Supplementary Material for this article can be found online at http://journal.frontiersin.org/article/10.3389/fendo.2017.00157/full#supplementary-material.

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Summary

Keywords

spondyloenchondrodysplasia, ACP5, growth hormone deficiency, growth hormone therapy, skeletal dysplasia

Citation

Utsumi T, Okada S, Izawa K, Honda Y, Nishimura G, Nishikomori R, Okano R and Kobayashi M (2017) A Case with Spondyloenchondrodysplasia Treated with Growth Hormone. Front. Endocrinol. 8:157. doi: 10.3389/fendo.2017.00157

Received

10 March 2017

Accepted

22 June 2017

Published

10 July 2017

Volume

8 - 2017

Edited by

Silvano Bertelloni, S. Chiara University-Hospital, Italy

Reviewed by

Benjamin Udoka Nwosu, University of Massachusetts Medical School, United States; Marek Niedziela, Poznan University of Medical Sciences, Poland

Updates

Copyright

*Correspondence: Takanori Utsumi,

Specialty section: This article was submitted to Pediatric Endocrinology, a section of the journal Frontiers in Endocrinology

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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