ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Thyroid Endocrinology
Volume 16 - 2025 | doi: 10.3389/fendo.2025.1537222
This article is part of the Research TopicThyroid Disorders Associated with Cancer ImmunotherapyView all articles
T3 and T4 Autoantibodies: Emerging Biomarkers for Evaluating Thyroid Disorders
Provisionally accepted
- Affiliated Hospital of Jiangsu University, Zhenjiang, China
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Introduction and objectives: The clinical significance of thyroid hormone autoantibodies, specifically triiodothyronine autoantibodies (T3-Ab) and thyroxine autoantibodies (T4-Ab), is not well understood due to current detection method limitations. This study investigated the clinical utility of T3-Ab and T4-Ab as biomarkers for thyroid function by developing a Magnetic Chemiluminescent Immunoassay (MCLIA) kit. Methods: A chemiluminescent immunoassay kit was developed using magnetic nanomicroparticles conjugated with T3 or T4 antigens. An indirect detection approach (magnetic microparticle antigen-target antibody-anti-human IgG antibody) was employed. Reference ranges were established using 415 serum samples from healthy individuals. Additionally, serum samples from 1,654 patients with various diseases were analyzed for T3-Ab and T4-Ab distribution levels and positive rates. Mass spectrometry and recovery experiments assessed potential interference of T3-Ab and T4-Ab with thyroid hormone detection. Results: The validation process confirmed the efficacy of the MCLIA kit
Keywords: Thyroid hormone autoantibodies, Immune Checkpoint Blockade Immunotherapy, Development of Assay Kits, biomarker, Immunoassay
Received: 30 Nov 2024; Accepted: 27 May 2025.
Copyright: © 2025 Liu, Mao, Mao, Wang, Zheng, Dong and Mao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yufei Mao, Affiliated Hospital of Jiangsu University, Zhenjiang, China
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