ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Gut Endocrinology
Volume 16 - 2025 | doi: 10.3389/fendo.2025.1600015
Proteomics Reveals Periodontitis-Driven Oxidative Stress and Lipid Metabolism Disruption in NAFLD
Provisionally accepted
- Department of Stomatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
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Background: Periodontitis is increasingly recognized as a risk factor for non-alcoholic fatty liver disease (NAFLD), yet the underlying mechanisms remain unclear. Oxidative stress and lipid metabolism dysregulation may serve as key pathological links. This study investigates the impact of periodontitis on NAFLD progression and identifies molecular targets involved in hepatic oxidative stress and lipid alterations.A rat model of periodontitis was established via molar ligation. Hepatic pathology was assessed using histological staining, biochemical assays, and oxidative stress markers.Proteomic analysis identified differentially expressed proteins (DEPs) associated with lipid metabolism and inflammation. Functional enrichment analysis, protein-protein interaction (PPI) network construction, and hub proteins identification were conducted.Results: Periodontitis significantly exacerbated hepatic lipid accumulation, fibrosis, and oxidative stress. Proteomic analysis identified 244 DEPs enriched in metabolic and inflammatory pathways. PPI network analysis revealed ACOX1, DBT, ACAA2, and HADHA as hub proteins. Downregulation of these proteins correlated with impaired lipid oxidation and hepatocellular injury in periodontitis-induced NAFLD.Periodontitis accelerates NAFLD progression through oxidative stress and lipid metabolism dysfunction. ACOX1, DBT, ACAA2, and HADHA may serve as therapeutic targets.These findings highlight the importance of oral health in systemic metabolic disorders and suggest new intervention strategies.
Keywords: periodontitis1, NAFLD2, Oxidative Stress3, Lipid metabolism4, Proteomics5
Received: 25 Mar 2025; Accepted: 11 Aug 2025.
Copyright: © 2025 Sun, Liu, Jiang, Qi, Guan and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hongjiao Li, Department of Stomatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092, China
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