ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Experimental Endocrinology
Volume 16 - 2025 | doi: 10.3389/fendo.2025.1672823
This article is part of the Research TopicFunction of hormones, their receptors and binding proteinsView all 13 articles
Arginine-vasotocin is a player in the multifactorial control of spermatogenesis in zebrafish
Provisionally accepted
- 1Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, Canada
- 2University of Calgary Cumming School of Medicine, Calgary, Canada
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This study investigated the role of vasotocin (AVT) in the multifaceted regulation of spermatogenesis in zebrafish, with a focus on its interaction with gonadotropin hormones. Using an ex vivo cultured testis, we explored the interaction of AVT with gonadotropins, LH and FSH, to regulate different stages of germ cell development. In this study, we used recombinant zebrafish FSH and hCG to stimulate the LH-induced response due to the limited availability of zebrafish LH, as it is known to exert similar effects on testicular function. Treatment with AVT enhanced FSH-induced proliferation of early undifferentiated spermatogonia (Aund) germ cells, as well as promoting the proliferation of later-stage type B germ cells when combined with LH/hCG. Additionally, AVT significantly decreased FSH-induced 11-ketotestosterone (11-KT) production and Leydig-derived factors, including cyp17a1 and insl3, without significantly affecting LH/hCG-induced androgen production. Based on these findings, we hypothesize that in the presence of FSH, AVT drives early germ cell proliferation and differentiation while simultaneously inhibiting premature progression through spermiogenesis. This stage-specific modulation of gonadotropin signaling by AVT underscores its dual role in fine-tuning testicular function and germ cell maturation in male zebrafish. Overall, our findings suggest that AVT contributes to the complex multifactorial network regulating spermatogenesis in zebrafish.
Keywords: Vasotocin (AVT), Gonadotropin, Follicle-stimulating hormone (FSH), Human chorionic gonadotropin (hCG), Luteinizing hormone (LH), Spermatogenesis, Zebrafish
Received: 25 Jul 2025; Accepted: 06 Oct 2025.
Copyright: © 2025 Habibi, Zanardini, Ma and Parker. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hamid R Habibi, habibi@ucalgary.ca
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