ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Reproduction
Volume 16 - 2025 | doi: 10.3389/fendo.2025.1682526
This article is part of the Research TopicFertility preservation in female cancer survivors: innovative strategies for fertility preservation and reproductive outcomesView all articles
Alpha-lipoic acid reduces oxidative damage and ameliorates follicular abnormalities in vitrified cat ovarian tissue
Provisionally accepted
- Yantai Yuhuangding Hospital, Yantai, China
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This study aims to investigate the effects of Alpha lipoic acid (α-ALA) on cat ovarian cortex vitrification. Since vitrification may induce oxidative damage, mitigating cryodamage is crucial for preserving follicular development and ovarian function. For this purpose, we established a α-ALA concentration gradients (0, 100, and 150 μM) in the vitrification solution, assessed follicular abnormalities, and analyzed cell apoptosis. Based on these results, 150 μM was selected for subsequent in vitro culture experiments. During vitrification, thawing, and culture, tissues were supplemented with either 0 or 150 μM α-ALA. Tissues from Fresh, vitrification solution (VS) only and VS + α-ALA (150 μM) were collected at culture days 0, 2, and 7 for immunohistochemical detection of caspase3, SOD1, and Ki67. Additionally, Masson staining was conducted to compare the occurrence of fibrosis. Our findings demonstrated that 150 μM α-ALA delayed cell apoptosis, enhanced cell proliferation, and attenuated the fibrotic process. Moreover, we conducted transcriptome sequencing and identified that vitrification induced changes in cytoskeletal regulation and chromosome segregation, while α-ALA could partially ameliorate these alterations. Therefore, it is concluded that α-ALA effectively mitigates vitrification correlated ovarian functional impairment.
Keywords: Ovary, Vitrification, Oxidative damage, Follicle development, Transcriptome sequencing
Received: 09 Aug 2025; Accepted: 24 Sep 2025.
Copyright: © 2025 Yin, Li, Pan, Bao and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongchu Bao, baohongchu@163.com
Xiaoyan Liu, 9712lxy@163.com
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