Abstract
Although anti-C1q autoantibodies have been described more than four decades ago a constant stream of papers describing clinical associations or functional consequences highlights that anti-C1q antibodies are still hot and happening. By far the largest set of studies focus on anti-C1q antibodies is systemic lupus erythematosus (SLE). In SLE anti-C1q antibodies associate with involvement of lupus nephritis in such a way that in the absence of anti-C1q antibodies it is unlikely that a flare in nephritis will occur. Anti-C1q antibodies occur in several autoimmune conditions but also in healthy individuals. Although considerable progress has been made in the understanding of how anti-C1q antibodies may contribute to tissue injury there is still a lot to learn about the processes involved in the breaking of tolerance to this protein. There has been considerable improvement in the assays employed to test for the presence of anti-C1q antibodies. Hopefully with these new and standardized assays at hand larger clinical association studies will be conducted with independent replication. Such large-scale studies will reveal the true value of clinical testing for anti-C1q autoantibodies in several clinical conditions.
Introduction
Of all the autoantibodies that target complement proteins, anti-C1q autoantibodies have received most attention (Trouw et al., ; Norsworthy and Davies, ). C1q, the initiation molecule of the classical pathway of complement activation, has a unique capacity to bind to the Fc tail of subclasses of IgG and IgM antibodies (Daha et al., ). C1q does so only when at least two IgG molecules are spatially oriented in such a way that they can simultaneously interact with one C1q molecule as for example in an immune-complex (Cooper, ). Alternatively, C1q can bind to a single IgM molecule in a “staple” like configuration (Feinstein et al., ). The binding of C1q to non-aggregated IgG molecules or fluid-phase IgM is weak. The fact that C1q can bind IgG in immune complexes can be considered as both a blessing and a curse. The blessing lies in the fact that the identification of anti-C1q antibodies was a consequence of studies on size fractionations of immune complexes that could bind to C1q. In these studies it was subsequently discovered that in systemic lupus erythematosus (SLE) patients next to the high molecular weight fractions also low-molecular weight fractions contained immunoglobulins that could bind to C1q (Agnello et al., ) (Table 1). In the following years these low-molecular weight fractions were further identified as monomeric, non-complexed IgG molecules that specifically interacted with the collagen-like tail of the C1q molecule (Uwatoko et al., , ; Antes et al., ). The curse lies in the fact that special care has to be taken to discriminate between IgG in immune complexes binding to C1q and anti-C1q autoantibodies binding to C1q (Kohro-Kawata et al., ). This problem can be overcome by adding 1 M NaCl to the incubation buffer in the assay. The low-avidity interactions between the C1q globular head domains with the CH2 domains of IgG Fc tails is completely disrupted in these conditions, whereas the high avidity binding of anti-C1q autoantibodies to the collagen-like tail of C1q is kept intact (Kohro-Kawata et al., ). It is currently not known to what extend the high salt conditions may lead to an underestimation of low-avidity anti-C1q autoantibodies.
Table 1
| Year | Milestone | Reference |
|---|---|---|
| 1971/88 | Identification of C1q as the target of autoantibodies | Agnello et al. (), Antes et al. () |
| 1982 | C1q in solid-phase exposes neo-epitopes | Golan et al. () |
| 1984 | Identification of the collagen-like stalk as the main binding site of anti-C1q antibodies | Uwatoko et al. (, ) |
| 1987 | Anti-C1q associates with the occurrence of LN | Wener et al. (); reviewed a. o. Trendelenburg () |
| 1991 | C1q and anti-C1q are enriched in the glomeruli of LN | Uwatoko et al. () |
| 1996 | Identification of anti-C1q in mice | Hogarth et al. () |
| 1993 | Anti-C1q also present in healthy population and increase with age | Siegert et al. () |
| 2004 | Experimental evidence on how anti-C1q can be pathogenic to the kidney in LN but not in healthy individuals | Trouw et al. () |
| 2007 | Identification of anti-C1q antibodies that target the globular heads | Tsacheva et al. () |
History of anti-C1q antibodies.
Assays to Detect Anti-C1q Autoantibodies
Over time several assays have been developed to detect anti-C1q autoantibodies both in humans and in experimental animal models. The first assays employed a direct coating of intact C1q, which necessitated the use of high salt conditions to discriminate between immune-complex binding and anti-C1q autoantibody binding (Kohro-Kawata et al., ). Already early in the history of anti-C1q autoantibodies it was discovered that the majority of these autoantibodies is directed against the collagen-like part of the C1q molecule (Antes et al., ). From equilibrium studies and from the observation that anti-C1q antibodies can be found in the presence of freely circulating C1q it was argued that anti-C1q antibodies may interact with epitopes that are not exposed in C1q in fluid phase (Golan et al., ). Later these arguments were supported by elegant studies using phage display technology generated Fab fragments that only interacted with solid-phase C1q (Schaller et al., ). Next, assays have been developed that utilized only the C1q collagen-like region, generated by enzymatic digestions as antigen (Antes et al., ; Wener et al., ). This eliminated the need to use high-ionic strength buffer. A recent paper reports on the use of peptides derived from C1q that have interesting properties to detect a major linear epitope in a high percentage of the patients in the absence of high-ionic strength buffer (Vanhecke et al., ). In contrast to the assays reported before that anti-C1q antibodies only target the collagen-like region of C1q in 2007 it was discovered that there are also antibodies that specifically target the globular head regions of C1q (Tsacheva et al., ).
To study anti-C1q antibodies in experimental animal models, assays were developed that used coating of purified mouse C1q and high salt conditions similar to the human situation (Hogarth et al., ; Trouw et al., ). Next, in order to circumvent the purification of mouse C1q, an assay was developed which employed a coating of C1q binding peptides, that captured C1q from Rag−/− serum, as the antigenic entity for the anti-C1q ELISA (Trouw et al., ,).
Several commercial assays are available for the detection of anti-C1q antibodies (see Table 2). Those assays are mostly based on the ELISA technology and are marketed by Bühlmann Laboratories AG (CH-4124 Schönenbuch, Switzerland), IMTEC (HUMAN, Wiesbaden, Germany), Orgentec (Mainz, Germany), and INOVA Diagnostics (San Diego, CA, USA). The ALEGRIA system is a semi-automated assay system based on patient specific modified microtiter plate strips. Some of the anti-C1q antibody assays have been used in clinical studies (Trendelenburg et al., ; Potlukova and Kralikova, ; Heidenreich et al., ; Meyer et al., ; Cai et al., ; Akhter et al., ; Julkunen et al., ). Until today, none of the anti-C1q antibody assays achieved clearance by the Food and Drug Administration (FDA) due to the lack of prospective studies. In addition, systematic studies comparing anti-C1q antibody assays from different companies are missing.
Table 2
| Trade name (P/N) | Company | Technology | Reference |
|---|---|---|---|
| Anti-C1q autoantibody ELISA (EK-AC1QA) | Buehlmann Laboratories AG | ELISA | Trendelenburg et al. (), Potlukova et al. (), Meyer et al. (), Julkunen et al. () |
| IMTEC-anti-C1q-antibodies (ITC59033) | IMTEC | ELISA | Cai et al. () |
| QUANTA lite anti-C1q (704565) | INOVA Diagnostics | ELISA | Akhter et al. () |
| Anti-C1q (ORG 549) | Orgentec | ELISA | Heidenreich et al. (), Julkunen et al. () |
| Anti-C1q (ORG 249) | Orgentec | Alegria | NA |
Overview of commercially available assays for the detection of anti-C1q antibodies.
Occurrence of Anti-C1q Autoantibodies and Clinical Associations
Over the past four decades anti-C1q autoantibodies have been studied in a wide variety of autoimmune and renal conditions as well as in infectious diseases (Trendelenburg, ). In the healthy population the prevalence of anti-C1q autoantibodies ranges between 2 and 8% (Wener et al., ; Siegert et al., ; Trendelenburg et al., ; Horvath et al., ; Potlukova et al., ) and increases with age (Siegert et al., ). Hypocomplementemic Urticarial Vasculitis Syndrome (HUVS) represents the clinical condition with the highest percentage of anti-C1q positivity; 100% (Wisnieski and Jones, ). Other conditions characterized by high anti-C1q antibody prevalence are, mixed connective tissue disease (94%), Felty’s syndrome (76%), and SLE (30–60%) (Siegert et al., ; Trendelenburg, ; Potlukova et al., ; Sinico et al., ). The occurrence of anti-C1q autoantibodies was shown to have familial clustering, indicating that there is a genetic risk factor that together with environmental cues may precipitate the production of these antibodies (Hunnangkul et al., ). Anti-C1q autoantibodies have also been described to occur in infectious diseases although at a frequency of around, for example 13% of HIV infected individuals vs. 5% in healthy controls (Prohaszka et al., ) or up to 26% in patients suffering from hepatitis C virus infection as compared to 10% of healthy controls (Saadoun et al., ).
Especially the association between anti-C1q antibodies and renal involvement in SLE has received much attention (Seelen et al., ). Correct diagnosis of a flare of lupus nephritis (LN) still represents an important challenge. Serological identification of a flare would be preferred over repeated renal biopsy. Although several anti-nuclear antibodies are associated with renal involvement and active disease (Heidenreich et al., ), the presence of anti-C1q antibodies either alone or in combination with other serological markers is superior to predict/correlate with active LN as reviewed before (Trendelenburg, ; Sinico et al., ). Several studies provide evidence that anti-C1q autoantibodies are superior to other serological markers in identifying a flare of LN (Mok et al., ; Akhter et al., ). However, other studies indicate that combinations of anti-C1q antibodies with other serological markers are superior to anti-C1q antibodies alone (Matrat et al., ; Julkunen et al., ; Yang et al., ). Especially striking is the strong negative predictive value of anti-C1q testing for LN. In the absence of anti-C1q autoantibodies it is very unlikely that a patient with LN will develop a flare (Trendelenburg et al., , ; Meyer et al., ; Mok et al., ; Matrat et al., ; Moura et al., ). As many of these studies report on rather small patient populations from very diverse ethnic backgrounds ranging from Brazil (Moura et al., ), China (Zhang et al., ), India (Pradhan et al., ), and Egypt (ElGendi and El-Sherif, ) it is likely that considerable variation exists in the strength at which anti-C1q antibodies are associated with and is predictive for LN flares. Several of the larger studies from Europe and Hong Kong point in the same direction (Moroni et al., ; Mok et al., ; Julkunen et al., ) and also a recent meta-analysis confirmed the diagnostic value of serum anti-C1q antibodies for LN (Yin et al., ).
Whether or not anti-C1q antibodies are also associated with the disease activity of LN remains to be established as currently there is no consensus on this issue (Horvath et al., ; Grootscholten et al., ; Julkunen et al., ). One mechanism to clear anti-C1q autoantibodies from the circulation is to use immunoabsorption on C1q-columns (Hiepe et al., ). This method depleted next to circulating immune complexes also anti-C1q autoantibodies and was shown to be beneficial in SLE patients (Berner et al., ; Pfueller et al., ).
Anti-C1q Antibodies as Part of Multiplex Technologies
With the emerging availability of multiplex technologies for the detection of autoantibodies and other serological markers, the combination of anti-C1q antibodies with other markers is facilitated (Papp et al., ). Recent studies have demonstrated that biomarker profiles have the potential to improve the diagnosis of SLE (Kalunian et al., ).
This is important also in the light of new treatment opportunities for SLE (Stohl and Hilbert, ) and personalized medicine which seems to be just around the corner (Kalunian and Joan, ). The ultimate goal is to develop a panel of serological markers that are able to predict SLE flares which then can be prevented by initiating the appropriate treatment. Especially life threatening complications such as kidney failure and transplantation should be prevented. Recently it has been described that anti-chromatin (anti-nucleosome) antibodies are a promising serological marker to help to predict the need for kidney transplantation (Stinton et al., ). Whether testing for anti-C1q antibodies in a multiplex setting has the potential to contribute to the improved management of LN patients remains a matter of further research.
Pathogenic Consequences of Anti-C1q Autoantibodies
Several studies have addressed the mechanisms by which anti-C1q autoantibodies may contribute to tissue damage, especially in LN. Immune complexes eluted from affected glomeruli of human patients and experimental animals revealed that there is a strong enrichment of anti-C1q antibodies and that this deposition seemed to occur via solid-phase C1q (Uwatoko et al., ; Mannik and Wener, ; Trouw et al., ). Next to the previously mentioned clinical association studies also in vitro and in vivo animal studies have been performed (Siegert et al., ; Hogarth et al., ; Trouw et al., ,; Bigler et al., ). Several of the mouse models of lupus are characterized by a progressive autoimmune disease in which autoantibodies are generated, immune complexes are formed followed by the occurrence of severe glomerulonephritis. Depending on the mouse model these autoimmune phenomena may evolve in different degrees of severity and at different ages. Using MRL/lpr, BXSB, and NZB/W mice, with a severe lupus phenotype, it was demonstrated that anti-C1q autoantibodies are also present in mice and that an increase in the titer of anti-C1q antibodies are associated with the onset of nephritis (Hogarth et al., ; Trouw et al., ). Using a different model, using MRL/MpJ+/+ mice with a less severe lupus phenotype, it was concluded that glomerulonephritis may also occur in the absence of anti-C1q antibodies (Bigler et al., ). In a more experimental setting, injection of rabbit anti-mouse C1q antibodies resulted in immune-complex deposition of C1q and anti-C1q antibodies but the limited degree of deposition was insufficient to induce glomerulonephritis (Trouw et al., ). However, injection of mouse anti-mouse C1q autoantibodies into animals that have C1q containing immune complexes in the glomeruli, resulted in strong glomerulonephritis (Trouw et al., ). Collectively these data indicate that anti-C1q antibodies can be present in healthy subjects (mouse or human) which might induce limited deposition in the kidney but no nephritis. Only in the presence of C1q containing immune complexes in the kidney, anti-C1q autoantibodies will amplify the local complement activation and cellular influx resulting in glomerulonephritis. A similar process may also be operational in post-streptococcal glomerulonephritis where anti-C1q autoantibodies were also found to associate with a worse disease course (Kozyro et al., ). Why anti-C1q autoantibodies would predominantly enhance the tissue damage in glomeruli and not or less pronounced in other tissues known to contain immune complexes in lupus is currently unknown. The observation that anti-C1q autoantibodies may specifically target C1q bound to early-apoptotic cells (Bigler et al., ) raises the question what the in vivo consequences would be of enhanced complement activation on apoptotic cells. One possible scenario could be that the natural mechanisms that would limit excessive complement activation on dying cells would be overruled (Trouw et al., , ) resulting in lysis of the cells and exposure of autoantigenic components to the immune system. The observation that anti-C1q autoantibodies are also observed in autoimmune thyroid diseases and that their levels correlate with thyroid function (Potlukova et al., ) may suggest that the effect of anti-C1q antibodies amplifying immune-complex mediated damage only in the kidney is incomplete and that the presence of anti-C1q antibodies may enhance tissue damage in several other, unexpected clinical conditions.
In conclusion; anti-C1q autoantibodies play an important role in the clinical management of LN. Testing for anti-C1q autoantibodies in large well defined cohorts of several diseases, preferable in a prospective study design, is likely to provide additional clinical conditions for which the testing for anti-C1q autoantibodies would have clinical implications.
Statements
Acknowledgments
We acknowledge the financial support from The Netherlands Organization for Scientific Research, Masterswitch project FP7, the IMI JU funded project BeTheCure, contract no 115142-2, INOVA Diagnostics Inc., The Netherlands Proteomics Center, and the Center for Medical Systems Biology as part of The Netherlands Genomics Initiative. Leendert A. Trouw is supported by a ZON-MW Vidi grant and by a fellowship from Janssen Biologics.
Conflict of interest
Dr. M. Mahler is employee of INOVA Diagnostics INC., an autoimmune diagnostics company that provides assays for autoantibody detection. He was invited by Dr. L.A. Trouw to participate because of his knowledge of the various commercial assays available for the detection of this autoantibody.
References
1
AgnelloV.KofflerD.EisenbergJ. W.WinchesterR. J.KundelH. G. (1971). C1g precipitins in the sera of patients with systemic lupus erythematosus and other hypocomplementemic states: characterization of high and low molecular weight types. J. Exp. Med.134, 228s–241s.
2
AkhterE.BurlingameR. W.SeamanA. L.MagderL.PetriM. (2011). Anti-C1q antibodies have higher correlation with flares of lupus nephritis than other serum markers. Lupus20, 1267–1274.10.1177/0961203311411597
3
AntesU.HeinzH. P.LoosM. (1988). Evidence for the presence of autoantibodies to the collagen-like portion of C1q in systemic lupus erythematosus. Arthritis Rheum.31, 457–464.10.1002/art.1780310401
4
BernerB.ScheelA. K.SchettlerV.HummelK. M.Reuss-BorstM. A.MullerG. A.et al (2001). Rapid improvement of SLE-specific cutaneous lesions by C1q immunoadsorption. Ann. Rheum. Dis.60, 898–899.
5
BiglerC.HopferH.DannerD.SchallerM.MihatschM. J.TrendelenburgM. (2011). Anti-C1q autoantibodies do not correlate with the occurrence or severity of experimental lupus nephritis. Nephrol. Dial. Transplant.26, 1220–1228.10.1093/ndt/gfq558
6
BiglerC.SchallerM.PerahudI.OsthoffM.TrendelenburgM. (2009). Autoantibodies against complement C1q specifically target C1q bound on early apoptotic cells. J. Immunol.183, 3512–3521.10.4049/jimmunol.0803573
7
CaiX.YangX.LianF.LinX.LiangM.LiJ.et al (2010). Correlation between serum anti-C1q antibody levels and renal pathological characteristics and prognostic significance of anti-C1q antibody in lupus nephritis. J. Rheumatol.37, 759–765.10.3899/jrheum.090779
8
CooperN. R. (1985). The classical complement pathway: activation and regulation of the first complement component. Adv. Immunol.37, 151–216.10.1016/S0065-2776(08)60340-5
9
DahaN. A.BandaN. K.RoosA.BeurskensF. J.BakkerJ. M.DahaM. R.et al (2011). Complement activation by (auto) antibodies. Mol. Immunol.48, 1656–1665.10.1016/j.molimm.2011.06.224
10
ElGendiS. S.El-SherifW. T. (2009). Anti-C1q antibodies, sCD40L, TWEAK and CD4/CD8 ratio in systemic lupus erythematosus and their relations to disease activity and renal involvement. Egypt. J. Immunol.16, 135–148.
11
FeinsteinA.MunnE. A.RichardsonN. E. (1971). The three-dimensional conformation of M and A globulin molecules. Ann. N. Y. Acad. Sci.190, 104–121.10.1111/j.1749-6632.1971.tb13526.x
12
GolanM. D.BurgerR.LoosM. (1982). Conformational changes in C1q after binding to immune complexes: detection of neoantigens with monoclonal antibodies. J. Immunol.129, 445–447.
13
GrootscholtenC.DiekerJ. W.McGrathF. D.RoosA.DerksenR. H.van der VlagJ.et al (2007). A prospective study of anti-chromatin and anti-C1q autoantibodies in patients with proliferative lupus nephritis treated with cyclophosphamide pulses or azathioprine/methylprednisolone. Ann. Rheum. Dis.66, 693–696.10.1136/ard.2006.065425
14
HeidenreichU.MayerG.HeroldM.KlotzW.Stempfl Al-JazrawiK.LhottaK. (2009). Sensitivity and specificity of autoantibody tests in the differential diagnosis of lupus nephritis. Lupus18, 1276–1280.10.1177/0961203309345753
15
HiepeF.PfullerB.WolbartK.BrunsA.LeinenbachH. P.HepperM.et al (1999). C1q: a multifunctional ligand for a new immunoadsorption treatment. Ther. Apher.3, 246–251.
16
HogarthM. B.NorsworthyP. J.AllenP. J.TrinderP. K.LoosM.MorleyB. J.et al (1996). Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice. Clin. Exp. Immunol.104, 241–246.10.1046/j.1365-2249.1996.19725.x
17
HorvathL.CzirjakL.FeketeB.JakabL.ProhaszkaZ.CervenakL.et al (2001a). Levels of antibodies against C1q and 60 kDa family of heat shock proteins in the sera of patients with various autoimmune diseases. Immunol. Lett.75, 103–109.10.1016/S0165-2478(00)00287-X
18
HorvathL.CzirjakL.FeketeB.JakabL.PozsonyiT.KalabayL.et al (2001b). High levels of antibodies against Clq are associated with disease activity and nephritis but not with other organ manifestations in SLE patients. Clin. Exp. Rheumatol.19, 667–672.
19
HunnangkulS.NitschD.RhodesB.ChadhaS.RobertonC. A.Pessoa-LopesP.et al (2008). Familial clustering of non-nuclear autoantibodies and C3 and C4 complement components in systemic lupus erythematosus. Arthritis Rheum.58, 1116–1124.10.1002/art.23400
20
JulkunenH.Ekblom-KullbergS.MiettinenA. (2012). Nonrenal and renal activity of systemic lupus erythematosus: a comparison of two anti-C1q and five anti-dsDNA assays and complement C3 and C4. Rheumatol. Int.32, 2445–2451.10.1007/s00296-011-1962-3
21
KalunianK.JoanT. M. (2009). New directions in the treatment of systemic lupus erythematosus. Curr. Med. Res. Opin.25, 1501–1514.10.1185/03007990902929104
22
KalunianK. C.ChathamW. W.MassarottiE. M.Reyes-ThomasJ.HarrisC.FurieR. A.et al (2012). Measurement of cell-bound complement activation products enhances diagnostic performance in systemic lupus erythematosus. Arthritis Rheum.64, 4040–4047.10.1002/art.34669
23
Kohro-KawataJ.WenerM. H.MannikM. (2002). The effect of high salt concentration on detection of serum immune complexes and autoantibodies to C1q in patients with systemic lupus erythematosus. J. Rheumatol.29, 84–89.
24
KozyroI.KorostelevaL.ChernoshejD.DannerD.SukaloA.TrendelenburgM. (2008). Autoantibodies against complement C1q in acute post-streptococcal glomerulonephritis. Clin. Immunol.128, 409–414.10.1016/j.clim.2008.04.005
25
MannikM.WenerM. H. (1997). Deposition of antibodies to the collagen-like region of C1q in renal glomeruli of patients with proliferative lupus glomerulonephritis. Arthritis Rheum.40, 1504–1511.10.1002/art.1780400819
26
MatratA.Veysseyre-BalterC.TrollietP.VillarE.DijoudF.BienvenuJ.et al (2011). Simultaneous detection of anti-C1q and anti-double stranded DNA autoantibodies in lupus nephritis: predictive value for renal flares. Lupus20, 28–34.10.1177/0961203310379871
27
MeyerO. C.Nicaise-RolandP.CadoudalN.Grootenboer-MignotS.PalazzoE.HayemG.et al (2009). Anti-C1q antibodies antedate patent active glomerulonephritis in patients with systemic lupus erythematosus. Arthritis Res. Ther.11, R87.10.1186/ar2725
28
MokC. C.HoL. Y.LeungH. W.WongL. G. (2010). Performance of anti-C1q, antinucleosome, and anti-dsDNA antibodies for detecting concurrent disease activity of systemic lupus erythematosus. Transl. Res.156, 320–325.10.1016/j.trsl.2010.07.009
29
MoroniG.TrendelenburgM.DelP. N.QuagliniS.RaschiE.PanzeriP.et al (2001). Anti-C1q antibodies may help in diagnosing a renal flare in lupus nephritis. Am. J. Kidney Dis.37, 490–498.10.1053/ajkd.2001.22071
30
MouraC. G.MangueiraC. L.CruzL. A.CruzC. M. (2011). Negative anti-C1q antibody titers may influence therapeutic decisions and reduce the number of renal biopsies in systemic lupus erythematosus. Nephron Clin. Pract.118, c355–c360.10.1159/000323388
31
NorsworthyP.DaviesK. A. (2003). Complement components and their autoantibodies. Mol. Biotechnol.23, 259–270.10.1385/MB:23:3:259
32
PappK.VeghP.HoborR.SzittnerZ.VokoZ.PodaniJ.et al (2012). Immune complex signatures of patients with active and inactive SLE revealed by multiplex protein binding analysis on antigen microarrays. PLoS ONE7:e44824.10.1371/journal.pone.0044824
33
PfuellerB.WolbartK.BrunsA.BurmesterG. R.HiepeF. (2001). Successful treatment of patients with systemic lupus erythematosus by immunoadsorption with a C1q column: a pilot study. Arthritis Rheum.44, 1962–1963.10.1002/1529-0131(200108)44:8<1962::AID-ART335>3.0.CO;2-R
34
PotlukovaE.JiskraJ.LimanovaZ.KralikovaP.SmutekD.MareckovaH.et al (2008). Autoantibodies against complement C1q correlate with the thyroid function in patients with autoimmune thyroid disease. Clin. Exp. Immunol.153, 96–101.10.1111/j.1365-2249.2008.03670.x
35
PotlukovaE.KralikovaP. (2008). Complement component c1q and anti-c1q antibodies in theory and in clinical practice. Scand. J. Immunol.67, 423–430.10.1111/j.1365-3083.2008.02089.x
36
PradhanV.RajadhyakshaA.MahantG.SurveP.PatwardhanM.DigheS.et al (2012). Anti-C1q antibodies and their association with complement components in Indian systemic lupus erythematosus patients. Indian J. Nephrol.22, 353–357.10.4103/0971-4065.103911
37
ProhaszkaZ.DahaM. R.SusalC.DanielV.SzlavikJ.BanhegyiD.et al (1999). C1q autoantibodies in HIV infection: correlation to elevated levels of autoantibodies against 60-kDa heat-shock proteins. Clin. Immunol.90, 247–255.10.1006/clim.1998.4620
38
SaadounD.SadallahS.TrendelenburgM.LimalN.SeneD.PietteJ. C.et al (2006). Anti-C1q antibodies in hepatitis C virus infection. Clin. Exp. Immunol.145, 308–312.10.1111/j.1365-2249.2006.03153.x
39
SchallerM.BiglerC.DannerD.DitzelH. J.TrendelenburgM. (2009). Autoantibodies against C1q in systemic lupus erythematosus are antigen-driven. J. Immunol.183, 8225–8231.10.4049/jimmunol.0902642
40
SeelenM. A.TrouwL. A.DahaM. R. (2003). Diagnostic and prognostic significance of anti-C1q antibodies in systemic lupus erythematosus. Curr. Opin. Nephrol. Hypertens.12, 619–624.10.1097/00041552-200311000-00008
41
SiegertC. E.DahaM. R.HalmaC.van der VoortE. A.BreedveldF. C. (1992a). IgG and IgA autoantibodies to C1q in systemic and renal diseases. Clin. Exp. Rheumatol.10, 19–23.
42
SiegertC. E.DahaM. R.LobattoS.van der VoortE. A.BreedveldF. C. (1992b). IgG autoantibodies to C1q do not detectably influence complement activation in vivo and in vitro in systemic lupus erythematosus. Immunol. Res.11, 91–97.10.1007/BF02918613
43
SiegertC. E.DahaM. R.SwaakA. J.van der VoortE. A.BreedveldF. C. (1993). The relationship between serum titers of autoantibodies to C1q and age in the general population and in patients with systemic lupus erythematosus. Clin. Immunol. Immunopathol.67, 204–209.10.1006/clin.1993.1066
44
SinicoR. A.RimoldiL.RadiceA.BianchiL.GallelliB.MoroniG. (2009). Anti-C1q autoantibodies in lupus nephritis. Ann. N. Y. Acad. Sci.1173, 47–51.10.1111/j.1749-6632.2009.04746.x
45
StintonL. M.BarrS. G.TibblesL. A.YilmazS.SarA.BenedikttsonH.et al (2007). Autoantibodies in lupus nephritis patients requiring renal transplantation. Lupus16, 394–400.10.1177/0961203307078391
46
StohlW.HilbertD. M. (2012). The discovery and development of belimumab: the anti-BLyS-lupus connection. Nat. Biotechnol.30, 69–77.10.1038/nbt.2076
47
TrendelenburgM. (2005). Antibodies against C1q in patients with systemic lupus erythematosus. Springer Semin. Immunopathol.27, 276–285.10.1007/s00281-005-0007-y
48
TrendelenburgM.Lopez-TrascasaM.PotlukovaE.MollS.RegenassS.Fremeaux-BacchiV.et al (2006). High prevalence of anti-C1q antibodies in biopsy-proven active lupus nephritis. Nephrol. Dial. Transplant.21, 3115–3121.10.1093/ndt/gfl436
49
TrendelenburgM.MarfurtJ.GerberI.TyndallA.SchifferliJ. A. (1999). Lack of occurrence of severe lupus nephritis among anti-C1q autoantibody-negative patients. Arthritis Rheum.42, 187–188.10.1002/1529-0131(199901)42:1<187::AID-ANR24>3.0.CO;2-U
50
TrouwL. A.BengtssonA. A.GeldermanK. A.DahlbackB.SturfeltG.BlomA. M. (2007). C4b-binding protein and factor H compensate for the loss of membrane bound complement inhibitors to protect apoptotic cells against excessive complement attack. J. Biol. Chem.282, 28540–28548.10.1074/jbc.M704354200
51
TrouwL. A.BlomA. M.GasqueP. (2008). Role of complement and complement regulators in the removal of apoptotic cells. Mol. Immunol.45, 1199–1207.10.1016/j.molimm.2008.04.025
52
TrouwL. A.GroeneveldT. W.SeelenM. A.DuijsJ. M.BajemaI. M.PrinsF. A.et al (2004a). Anti-C1q autoantibodies deposit in glomeruli but are only pathogenic in combination with glomerular C1q-containing immune complexes. J. Clin. Invest.114, 679–688.10.1172/JCI200421075
53
TrouwL. A.SeelenM. A.VisserenR.DuijsJ. M.BenediktssonH.de HeerE.et al (2004b). Anti-C1q autoantibodies in murine lupus nephritis. Clin. Exp. Immunol.135, 41–48.10.1111/j.1365-2249.2004.02345.x
54
TrouwL. A.RoosA.DahaM. R. (2001). Autoantibodies to complement components. Mol. Immunol.38, 199–206.10.1016/S0161-5890(01)00043-8
55
TrouwL. A.SeelenM. A.DuijsJ. M.BenediktssonH.van KootenC.DahaM. R. (2003). Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of antimouse C1q antibodies. Clin. Exp. Immunol.132, 32–39.10.1046/j.1365-2249.2003.02108.x
56
TsachevaI.RadanovaM.TodorovaN.ArgirovaT.KishoreU. (2007). Detection of autoantibodies against the globular domain of human C1q in the sera of systemic lupus erythematosus patients. Mol. Immunol.44, 2147–2151.10.1016/j.molimm.2006.09.009
57
UwatokoS.AotsukaS.OkawaM.EgusaY.YokohariR.AizawaC.et al (1984). Characterization of C1q-binding IgG complexes in systemic lupus erythematosus. Clin. Immunol. Immunopathol.30, 104–116.10.1016/0090-1229(84)90011-4
58
UwatokoS.AotsukaS.OkawaM.EgusaY.YokohariR.AizawaC.et al (1987). C1q solid-phase radioimmunoassay: evidence for detection of antibody directed against the collagen-like region of C1q in sera from patients with systemic lupus erythematosus. Clin. Exp. Immunol.69, 98–106.
59
UwatokoS.GauthierV. J.MannikM. (1991). Autoantibodies to the collagen-like region of C1Q deposit in glomeruli via C1Q in immune deposits. Clin. Immunol. Immunopathol.61, 268–273.10.1016/S0090-1229(05)80030-3
60
VanheckeD.RoumeninaL. T.WanH.OsthoffM.SchallerM.TrendelenburgM. (2012). Identification of a major linear C1q epitope allows detection of systemic lupus erythematosus anti-C1q antibodies by a specific peptide-based enzyme-linked immunosorbent assay. Arthritis Rheum.64, 3706–3714.10.1002/art.34605
61
WenerM. H.MannikM.SchwartzM. M.LewisE. J. (1987). Relationship between renal pathology and the size of circulating immune complexes in patients with systemic lupus erythematosus. Medicine (Baltimore)66, 85–97.
62
WenerM. H.UwatokoS.MannikM. (1989). Antibodies to the collagen-like region of C1q in sera of patients with autoimmune rheumatic diseases. Arthritis Rheum.32, 544–551.10.1002/anr.1780320506
63
WisnieskiJ. J.JonesS. M. (1992). Comparison of autoantibodies to the collagen-like region of C1q in hypocomplementemic urticarial vasculitis syndrome and systemic lupus erythematosus. J. Immunol.148, 1396–1403.
64
YangX. W.TanY.YuF.ZhaoM. H. (2012). Combination of anti-C1q and anti-dsDNA antibodies is associated with higher renal disease activity and predicts renal prognosis of patients with lupus nephritis. Nephrol. Dial. Transplant.27, 3552–3559.10.1093/ndt/gfs179
65
YinY.WuX.ShanG.ZhangX. (2012). Diagnostic value of serum anti-C1q antibodies in patients with lupus nephritis: a meta-analysis. Lupus21, 1088–1097.10.1177/0961203312451202
66
ZhangC. Q.RenL.GaoF.MuF. Y.YouY. Q.LiuY. H. (2011). Anti-C1q antibodies are associated with systemic lupus erythematosus disease activity and lupus nephritis in northeast of China. Clin. Rheumatol.30, 967–973.10.1007/s10067-010-1660-7
Summary
Keywords
C1q, complement, autoantibody, diagnosis, SLE
Citation
Mahler M, van Schaarenburg RA and Trouw LA (2013) Anti-C1q Autoantibodies, Novel Tests, and Clinical Consequences. Front. Immunol. 4:117. doi: 10.3389/fimmu.2013.00117
Received
02 April 2013
Accepted
30 April 2013
Published
14 May 2013
Volume
4 - 2013
Edited by
Uday Kishore, Brunel University, UK
Reviewed by
Arvind Sahu, National Centre for Cell Science, India; Nicole Thielens, CNRS-CEA-Université Joseph Fourier, France
Copyright
© 2013 Mahler, van Schaarenburg and Trouw.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
*Correspondence: Leendert A. Trouw, Department of Rheumatology, C1R Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, Netherlands. e-mail: l.a.trouw@lumc.nl
This article was submitted to Frontiers in Molecular Innate Immunity, a specialty of Frontiers in Immunology.
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