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REVIEW article

Front. Immunol., 29 June 2023
Sec. T Cell Biology
This article is part of the Research Topic Global Excellence in Translational Immunology: Asia and Australasia 2023 View all 7 articles

Interplay between metabolic reprogramming and post-translational modifications: from glycolysis to lactylation

Hengwei Wu,,,Hengwei Wu1,2,3,4He Huang,,,*He Huang1,2,3,4*Yanmin Zhao,,,*Yanmin Zhao1,2,3,4*
  • 1Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
  • 2Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang, China
  • 3Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, People's Government of Zhejiang Province, Hangzhou, Zhejiang, China
  • 4Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou, Zhejiang, China

Cellular metabolism plays a critical role in determining the fate and function of cells. Metabolic reprogramming and its byproducts have a complex impact on cellular activities. In quiescent T cells, oxidative phosphorylation (OXPHOS) is the primary pathway for survival. However, upon antigen activation, T cells undergo rapid metabolic reprogramming, characterized by an elevation in both glycolysis and OXPHOS. While both pathways are induced, the balance predominantly shifts towards glycolysis, enabling T cells to rapidly proliferate and enhance their functionality, representing the most distinctive signature during activation. Metabolic processes generate various small molecules resulting from enzyme-catalyzed reactions, which also modulate protein function and exert regulatory control. Notably, recent studies have revealed the direct modification of histones, known as lactylation, by lactate derived from glycolysis. This lactylation process influences gene transcription and adds a novel variable to the regulation of gene expression. Protein lactylation has been identified as an essential mechanism by which lactate exerts its diverse functions, contributing to crucial biological processes such as uterine remodeling, tumor proliferation, neural system regulation, and metabolic regulation. This review focuses on the metabolic reprogramming of T cells, explores the interplay between lactate and the immune system, highlights the impact of lactylation on cellular function, and elucidates the intersection of metabolic reprogramming and epigenetics.

1 Introduction

The Nobel Prize in Physiology or Medicine 2019 was jointly awarded to three scientists for their discovery of how cells sense and adjust to oxygen levels. Oxygen sensing and energy metabolism play vital roles in numerous physiological and pathological processes. Tumors exhibit a unique energy metabolism known as the Warburg effect, where they preferentially use glycolysis to produce energy and accumulate substantial amounts of lactate, even under aerobic conditions. The Warburg effect is not exclusive to tumors but also occurs in several other diseases, such as sepsis, autoimmune disorders, atherosclerosis, diabetes, and ageing. Quiescent T cells primarily utilize oxidative phosphorylation (OXPHOS) for energy supply. However, upon antigen activation, these cells undergo rapid metabolic reprogramming and switch to aerobic glycolysis. This shift in metabolism supports T cell proliferation and enhances their functionality, representing a significant metabolic change during activation (1). For a long time, the accumulated lactate produced from glycolysis was viewed as a mere cellular energy source and metabolic byproduct, with its critical regulatory function in biological processes receiving inadequate attention. Lactate acts not only as a significant signaling molecule but also as a substrate for post-translational protein modification (2). As a ubiquitous metabolite, lactate still requires comprehensive elucidation of its role in physiological and pathological processes through post-translational modifications. This review will center on the critical biological process of metabolic reprogramming in immune cells, followed by an investigation of lactate’s effects on cellular activity

2 Metabolic reprogramming is vital for immune activities

Metabolic reprogramming is an adaptive modulation of cellular energy requirements to enable new functions in response to distinct environmental conditions. The Warburg effect is the archetypical example of metabolic reprogramming, wherein proliferating tumor cells metabolize glucose at a heightened rate via glycolysis, even under aerobic conditions, ultimately producing lactate. This phenomenon is widespread in many types of tumors (3, 4). Macrophages exhibit high plasticity and their polarization is influenced by stimuli from their microenvironment, which drives them to acquire distinct functions. Typically, macrophages are categorized into M1 (pro-inflammatory) and M2 (anti-inflammatory) subsets based on their function. The M2 macrophage subset exhibits activation of OXPHOS and fatty acid oxidation (FAO), whereas M1 macrophages utilize aerobic glycolysis (2). The metabolic pathways of pro-inflammatory cells, such as CD4+ effector T cells, are primarily shaped by glycolysis. In contrast, anti-inflammatory T cells, such as memory CD8+ effector T cells and Treg cells, are typically supported by OXPHOS and FAO. This section highlights the critical significance of metabolic reprogramming by focusing on T cells.

2.1 Elevated glycolysis is indispensable for eliciting active and functional T cells

When T cells are activated through CD3/CD28 in vitro, they undergo division within the following 24 to 72 hours. Thus, the first 24 hours are considered a “window” to determine the metabolic reprogramming profile upon activation (5). During rapid differentiation and proliferation, T cells upregulate both OXPHOS and glycolysis (6). Of note, the increase in OXPHOS is transient and serves as a characteristic of early activated T cells (7). Therefore, there is a dramatic shift in glucose utilization towards aerobic glycolysis, which facilitates the activation of the pentose phosphate pathway (PPP) and increased consumption of glutamine. This metabolic transition has significant implications, such as retaining some extent of oxygen molecules for other vital cellular processes that require oxygen during hypoxia and producing metabolic intermediates necessary for maintaining cellular activity and generating daughter cells (8, 9). This close connection between T cell activation and metabolism suggests that changes in T cell metabolism are not just a result of activation, but a factor that influences T cell fate. For instance, CD4+ effector memory T cells rely on glycolysis to avoid apoptosis (10).

Active aerobic glycolysis is a lineage-decisive step for T cells. The activation of the phosphoinositide 3-kinases (PI3K)/acetyl coenzyme A (Akt)/mammalian target of rapamycin (mTOR) pathway or the regulation by transcription factors Myc and hypoxia-induced factor 1 α (HIF-1α) can induce aerobic glycolysis in effector T cells. mTOR is composed of two complexes: mTOR complex 1 (mTORC1) and mTORC2. CD4+ T cells that lack mTORC1 cannot differentiate into Th1, Th2, or Th17 lineages (11). Stimulation of the T cell receptor (TCR) activates the PI3K/Akt/mTOR signaling pathway. mTOR activates HIF-1α and Myc to enhance glycolysis, glutaminolysis, and PPP (Figure 1) (5). The pathway plays a crucial role in regulating the expression and transport of glucose transporter 1 (GLUT1) (12). HIF-1α and Myc increase the expression of enzymes involved in glycolysis and glutaminolysis, as well as transporters for glucose and glutamine influx (1, 5, 13, 14). It is interesting to note that Myc-mediated glutamine catabolism works in concert with Myc-mediated glucose catabolism (5). This metabolic reprogramming not only aids in the differentiation of T cells towards effector T cells but also facilitates the formation of effector memory T cells (15). The metabolic activity and proliferation of cells create a hypoxic environment that leads to an increase in HIF-1α expression and further boosts glycolysis in low oxygen conditions (13, 16). HIF-1α reduces the differentiation of Th17 cells and enhances the differentiation of suppressive Treg cells (13). The Toll-like receptor (TLR) signals regulate the function of Treg cells. TLR1 and TLR2 promote glycolysis of Treg cells but impair suppression (17). But the deficient TLR adaptor-transducer MyD88 leads to a reduction of Treg cells in the gut, contributing to the development of inflammatory bowel disease (18).

FIGURE 1
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Figure 1 T cells undergo an increased uptake of glucose and glutamine to support the metabolic shift characterized by glycolysis, glutaminolysis and PPP. To sustain heightened glycolytic activity and ATP synthesis, prompt restoration of NAD+ from NADH is imperative. Hence, activated T cells facilitate this process by efficiently converting pyruvate, the final product of glycolysis, into lactate. 3-PG, 3-phosphoglyceric acid; HIF-1α, hypoxia-induced factor 1 alpha; Akt, acetyl coenzyme A; ASCT2, alanine serine cysteine transporter 1; Foxp3, forkhead box protein p3; GLUT, glucose transporter; HK2, hexokinase 2; LDHA, lactate dehydrogenase A; MCT, monocarboxylate transporter; mTORC1, mammalian target of rapamycin complex 1; OXPHOS, oxidative phosphorylation; PI3K, phosphoinositide 3-kinases; PPP, pentose phosphate pathway; SLC, solute carrier transporter; TCA cycle, tricarboxylic acid cycle; TCR, T cell receptor.

Maintaining glycolysis is crucial for the production of interferon-γ (IFN-γ). The expression of lactate dehydrogenase A (LDHA) is increased after cellular reprogramming to aerobic glycolysis. T cells with a deficiency of LDHA (LhdaKO T cells) have decreased IFN-γ expression, which ameliorates autoimmune diseases without impacting the expression of T-bet (19, 20). LDHA plays a role in maintaining the level of acetyl-CoA to some extent. Acetyl-CoA serves as a substrate for histone acetylation and this process, in turn, promotes the transcription of Ifng (20). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme involved in glycolysis, also binds to the AU-rich region of the 3′ untranslated region of Ifng mRNA and hinders its translation (21). The relationships between high glycolysis and inflammation (22), infections (23), and autoimmune diseases (24) are well established. CD4+ T cells differentiation into Th1 and Th17 lineages require glycolysis, thus the inhibition of glycolysis has been shown to ameliorate autoimmune encephalitis (25). However, impaired T-cell glycolysis has been observed in some autoimmune diseases associated with T cell dysfunction. Reduced expression of the rate-limiting glycolytic enzyme, 6-phosphofructokinase-2/fructobisphosphatase-2 isoenzyme 3 (PFKFB3), has been observed in CD4+ T cells from patients with rheumatoid arthritis. This reduction in PFKFB3 leads to decreased glycolysis, shifting the flow of glucose towards PPP, thereby depleting intracellular reactive oxygen species (ROS). As a result, these T cells exhibit a reduced capacity for energy generation and biosynthesis via autophagy (26).

2.2 Other metabolic features orchestrate with glycolysis tailoring immune response

T cell subsets exhibit distinct metabolic requirements for proliferation, differentiation, and function (21). While most helper T cells (Th1, Th2, Th17) rely mainly on aerobic glycolysis, Treg cells display heterogeneous metabolic profiles, with a reliance on FAO and OXPHOS as their primary energy sources (2729). This does not negate the importance of glycolysis in Treg cell biology. Inhibition of Treg cell glycolysis impairs their suppressive activity and decreases Foxp3 expression by recruiting enolase-1 (an enzyme of the glycolytic enzyme pathway) to the Foxp3 motif to control variable splicing (30). In addition, tumor-associated Treg cells exhibit high glucose uptake and glycolysis (31), while Foxp3 suppression of glycolysis through Myc decreases PI3K/Akt/mTORC1 signaling and escalates OXPHOS (17, 32). Many studies have found that if activated T cells are to differentiate into Treg cells, they must restrict their glycolysis levels and undergo a metabolic shift towards OXPHOS (28, 33, 34). Therefore, T cells initially exist in an OXPHOS state, undergo increased glycolysis upon activation, and finally tilt the balance of their metabolic profile towards OXPHOS to generate Treg cells. This highlights the interplay between metabolic processes and their mutual regulation.

T cell activation results in increased expression of genes regulating fatty acid synthesis (FASN) (35). Acetyl-CoA carboxylase (ACC) 1 and ACC2 are rate-limiting enzymes in FASN (11). The development of Th17 cells depends on ACC1-mediated FASN to synthesize phospholipids for cell membranes (36). Inhibition of ACC1 impairs the generation of Th17, Th1, and Th2 cells and leads to the development of Foxp3+ Treg cells, which ameliorates experimental autoimmune encephalomyelitis (33). Conversely, intratumoral Treg cells primarily rely on FASN for proliferation and mature Treg cell generation, rather than fatty acids (FAs) uptake (31, 37). Activated Tregs cells exhibit a transcription profile characterized by increased glycolysis and lipid biosynthesis (31). Interestingly, cells require essential FAs from the environment despite utilizing de novo FASN (38). Intratumoral Treg cells upregulate fatty acid-binding proteins and CD36, key facilitators of FAs uptake (39, 40). Meanwhile, there is a robust correlation between FAO and long-lived memory T cells (41). It’s interesting to note that memory T cells utilize glucose absorption for FASN and subsequently FAO, instead of utilizing extracellular free fatty acids for FAO (42). Although an initial increase in FAO during T cell activation, inhibition of FAO does not impede the proliferation and function of activated effector T cells (1, 43). Stimulation of T cells by PD-1 signaling leads to a shift in metabolism from glycolysis to fatty acid oxidation, which may contribute to the suppressive effect of PD-1 on T cell function (44).

As aforementioned, T cell activation is coupled with upregulated PPP, but blocking PPP has little effect on CD4+ T cell proliferation and activation in vivo, suggesting alternative pathways may support these processes (5). Glutaminase (GLS) converts glutamine to glutamate to support the tricarboxylic acid (TCA) cycle and epigenetic regulation. GLS deficiency impairs early T cell activation and reduces Th17 differentiation while increasing T-bet-related Th1 differentiation through altered gene expression and chromatin accessibility (45). These findings highlight the rapid, flexible, and reversible metabolic reprogramming and accompanying epigenetic changes that occur during T cell activation and differentiation.

2.3 Implications of immunometabolism on T cell biology

To meet the energy and metabolic demands for rapid proliferation, tumor cells uptake large amounts of glucose for glycolysis, resulting in glucose depletion in the tumor microenvironment (TME) and thereby inhibiting T cell glycolysis and subsequent anti-tumor response (46). In addition to correcting the unfavorable environment for T cells in the TME, it is also possible to enhance their function by targeting T cells themselves. Pre-clinical studies have revealed that the differentiation of CD8+ T cells into memory T cells could enhance their anti-tumor (47) and anti-viral capabilities (48), primarily due to improved survival and proliferative capacities. It has been established that CD8+ memory T cells primarily rely on FAO for energy production. Deficiencies in FAO fail to generate sufficient numbers of CD8+ memory T cells (49), while overexpression of carnitine palmitoyltransferase 1a (a rate-limiting enzyme of FAO) increases the abundance of memory T cells (50). Conversely, a metabolic state characterized by high glycolytic activity hinders the functional development of CD8+ memory T cells, driving the cells towards an effector T cell phenotype and subsequent exhaustion; inhibiting the glycolytic metabolism of T cells enhances the formation of memory phenotype and augments their anti-tumor effects (51).

As previously mentioned, T-cell activation leads to an upregulation of glucose transporter proteins, enhancing glucose uptake. Studies have demonstrated that CD4+ T cells infected with human immunodeficiency virus 1 (HIV-1) exhibit increased glycolysis and upregulation of GLUT1 expression, directly facilitating viral replication (52). Furthermore, the human T lymphotropic virus even exploits GLUT1 as an entry receptor and upregulates GLUT1 expression to facilitate subsequent infection (53). HIV-1 controllers (HICs) from CD8+ T cells represent a rare subset of individuals with a remarkable innate capacity to suppress viral replication without therapeutic interventions (54). HICs exhibit a distinct characteristic of reduced glycolysis and memory phenotype (55). Interestingly, non-HIC CD8+ T cells, upon treatment, display heightened fatty acid uptake and enhanced OXPHOS, demonstrating an augmented ability to suppress HIV-1 (55). Studies have shown that inhibiting glycolysis with lactose or 2-DG can significantly suppress HIV-1 infection (56, 57). Hence, analogous to tumorigenesis, restraining T cell glycolysis and enhancing FAO and OXPHOS seem to facilitate the development of memory phenotypes and extend cellular survival, constituting a promising yet nascent field warranting further investigation.

3 The glycolytic metabolite lactate is an important signaling molecule that shapes the function of immune cells

Aerobic glycolysis plays a crucial role in providing rapid energy in the form of ATP and producing intermediates for biosynthesis. However, the claim has been debated, as the majority of glucose carbon (more than 90%) is secreted as lactate and alanine, leaving limited room for biosynthesis (58). Creatine kinase and adenylate kinase can also promptly produce ATP (59, 60). Metabolites that are more likely to regulate cellular activities are of particular interest (21, 61). Altered metabolism rate changes the levels of intermediates and metabolites, endowing these small molecules with signaling functions (62). Hence, it is important to explore the potential functional and survival advantages that small molecules from metabolic processes bring to cells.

Studies on the relationship between immune cells and energy metabolism have primarily explored the impact of metabolic pathways on the immune system. Recent findings have revealed metabolites such as lactate, acetyl-CoA, and succinate play a crucial role in immune regulation as signaling molecules. Cellular activity generates a multitude of metabolites with various functions, including the regulation of cytokine production through alterations in cellular redox status, influencing transcriptional processes through binding to cytokine initiators, and modulating transmembrane ion channels, cell migration, and differentiation (6365). Metabolites, therefore, serve as messengers in cell-cell communication and response to the microenvironment, transcending their original definition as mere metabolic intermediates.

3.1 Lactate derived from glycolysis

Lactate, a byproduct of glycolysis, is transported out of the cytoplasm and subsequently deposited in the extracellular space. The conversion of pyruvate to lactate or lactate to pyruvate is catalyzed by lactate dehydrogenases (LDHs), which exist as five tetrameric isoenzymes (A4B0, A3B1, A2B2, A1B3, and A0B4) composed of LDHA and LDHB subunits (66). LDHB has a higher affinity for lactate and converts it to pyruvate, while LDHA favors the reverse reaction. Even though immune cells can express LDHA and LDHB subunits, it is interesting to note that T cells primarily express A4B0, which is increased during activation (20).

Lactate and protons are transported via reversible monocarboxylate transporters (MCTs) and their transport direction is determined by the concentration gradients of both monocarboxylate ions and protons. Different MCTs can facilitate intercellular lactic acid transport, while sodium-coupled lactate (e.g., Na-lactate) transport is mediated by solute carrier (SLC) 5A8 or SLC5A12 (67). Notably, CD4+ and CD8+ T cells detect heightened lactate levels upon infiltration of inflammatory sites through SLC5A12 and MCT1 (SLC16A1) transporters, respectively (64).

Historically, lactate was viewed merely as a waste product of metabolism and a biomarker for critically ill patients, neglecting its role as a bioactive molecule with major impacts on immune cells in the microenvironment. Lactate accumulation in the microenvironment significantly impacts immune cells, both tissue-resident and infiltrating. Lactate accumulated milieu can help evade immunosurveillance by conferring immunosuppressive functions to macrophages and T cells or amplifying inflammatory signals in inflammatory diseases (32, 63, 64, 68, 69). In certain healthy and malignant tissues, lactate utilization even outperforms glucose utilization as an energy source (70, 71). Lactate also acts as a signaling molecule when it binds to G-protein-coupled receptor 81 (GPR81), which is involved in lipolysis (72) and cancer cell survival (73). However, it remains unclear if using lactate as a carbon source also supports its other functions.

3.2 Lactate exerts its function via a receptor-independent manner

Lactate exists in two protonated forms, lactic acid at low pH and sodium lactate at high pH (74). Under physiological conditions, the majority of lactic acid has a negative charge. In the TME, which is typically hyperlactic with a pH ranging from 6.0-6.5, lactate exists in an acidic form. The hyperlactic milieu impacts immune cell behavior by increasing the regulatory function of myeloid-derived suppressor cells, inhibiting monocyte differentiation into dendritic cells (DCs), and compromising their antigen-presenting ability (Figure 2) (7577). Tumor-associated DCs can be induced by high lactic acid in tumors, either alone or in combination with cytokines (77). Although tumor-associated macrophages (TAMs) exhibit different transcriptional and metabolic characteristics, they all have a high lactate intake. Lactate provides a supplementary carbon source for MHC-IIlow TAM but impairs the metabolic activity of MHC-IIhigh TAM (78).

FIGURE 2
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Figure 2 The tumor microenvironment is a hyperlactic and hypoxic milieu due to tumor proliferation. These conditions are inherently immune-suppressive, affecting various immune cells.

T cells detect extracellular lactate and regulate intracellular signals to maintain cell activity and homeostasis. The low pH of the TME caused by high lactic acid can lead to anergy in activated T cells and NK cells, characterized by decreased glycolysis and lactate efflux, cytotoxicity, and cytokine production (29, 32). Lactate interferes with the autophagic pathway. Naïve T cells are prone to undergo apoptosis due to a selective loss of focal adhesion kinase (FAK) family–interacting protein of 200 kDa (FIP200). Lactate suppresses FIP200 and light chain 3-II expression in naïve T cells to promote T cell apoptosis (79). In addition, lactate drives T-cell death by hindering p38 signaling protein phosphorylation and subsequently inhibits the production of IFN-γ, tumor necrosis factor α (TNF-α), and IL-2 (80).

In addition to influencing the survival of T cells, lactate affects IFN-γ production. Lactic acid instead of its sodium salt prevents CD8+ T and NK cells from upregulating the nuclear factor of activated T cells (NFAT), the transcription factors involved in IFN-γ transcription, leading to reduced IFN-γ production (68). Sodium lactate does not affect the IFN-γ production in CD4+ T cells (64). Upon the uptake of lactate, a reduced NAD+/NADH ratio occurs, leading to the induction and activation of NAD-dependent deacetylase SIRT1 expression (81). Then the activated SIRT1 deacetylates T-bet, a key transcription factor for Th1 differentiation, leading to its breakdown, thereby impeding Th1 differentiation (82).

The reduced NAD+/NADH also affects other NAD+-dependent enzymes including GAPDH and 3-phosphoglycerate dehydrogenase, which not only affects glycolysis but also decreases the glycolytic intermediates such as phosphoenolpyruvate and serine which are necessary for T cell proliferation (83). Leukemia cell-derived lactate interferes with T cell function and proliferation by lowering intracellular pH, which reduces transcription of glycolysis-related enzymes in CD8+ T cells from acute myeloid leukemia patients who relapsed after hematopoietic stem cell transplant (84).

But the impact of lactate on CD8+ T cells in the TME has been a subject of debate. Recently, Qiang Feng et al. found that lactate enhances the stemness of CD8+ T cells in tumors, leading to reduced tumor growth and improved antitumor activity when administered to tumor-bearing mice (85). This inconsistency may be due to the use of two different types of lactate: lactic acid and sodium lactate, which have distinct properties. The presence of hydrogen ions in the TME consistently affects the dynamics of lactate and sodium lactate, leading to potential inconsistencies in many studies. A recent study found that CD8+ T cells exhibit elevated levels of granzyme B and IFN-γ in response to a neutral sodium lactate environment; however, acidic lactate at the same concentration was found to be detrimental to CD8+ T cell survival (preprint, DOI: 10.1101/2021.12.14.472728). Similar to this research, sodium lactate increased the number of cytokines produced by T cells (such as IFN-γ, IL-2, and TNF-α), which slowed tumor development (86). Of note, either sodium lactate or lactic acid always benefits Treg cells. High levels of Foxp3 expression drive the metabolic flux of Treg cells, making them more tolerant to a low-sugar, high-lactate condition by downregulating c-Myc and glycolysis, improving OXPHOS and increasing NAD+ oxidation (32). High glucose levels impair Treg cell function and stability, whereas lactate influx and intracellular production, regulated by MCT1 (SLC16A1), are essential for maintaining Treg cell inhibitory activity (29).

The inflammatory disease microenvironment is another hyperlactic milieu, with the most inflammatory sites displaying hypoxia. Lactate functions through protein binding and stabilizes prolonged hypoxia (24 hours under 1% O2), independent of the classical regulator HIF-1α. In normoxia, the N-myc downstream-regulated gene 3 (NDRG3) is degraded via PHD2/VHL-dependent manner, but NDRG3 prevents degradation by binding to lactate, thereby activating the Raf-ERK pathway and promoting angiogenesis and cell growth (87). The mitochondrial antiviral-signaling protein (MAVS) functions as a lactate sensor. Direct binding of lactate to MAVS results in its inactivation, which then limits the activation of the RIG-I-like receptor (RLR) signaling pathway. Consequently, there is a decrease in the production of type I interferon in T cells, leading to prolonged inflammation and impaired T cell function (88). Inflammatory areas are characterized by increased lactate transporter activity in T cells, which results in elevated cytokine production but impairs migratory capacity (64, 89). Exposure to sodium lactate decreases glucose flux and reduces the expression of several glycolytic enzymes in CD4+ T cells, impairing their ability to leave inflamed tissue (64). In rheumatoid arthritis, CD4+ T cells exhibit compromised glycolysis, reduced levels of ROS, energy deprivation, and autophagy deficiency (26). CD4+ T cells express the lactate transporter SLC5A12, which is responsible for lactate sensing. SLC5A12-mediated lactate uptake stimulates IL-17 production through the activation of the nuclear PKM2/STAT3 pathway and increases fatty acid synthesis in CD4+ T cells; inhibition of SLC5A12 reduces lactate uptake and improves T cell function, offering a potential therapeutic strategy for the treatment of arthritis (89).

3.3 Lactate mediates regulatory function in a receptor-dependent manner

Lactate is a natural ligand for GPR81, activating the receptor (73). By activating AMP-activated protein kinase (AMPK) and large tumor suppressor kinases, lactate-GPR81 signaling in macrophages suppresses YAP and NF-κB subunit p65 translocation, interaction, and activation (90). This lactate-GPR81 signaling pathway in macrophages also downregulates the NOD-like receptor protein 3 (NLRP3) inflammasome by interfering with caspase 1 and IL-1β release, thereby alleviating conditions such as immune hepatitis, acute pancreatitis, and acute liver injury (91). Notably, lactate exerts its suppressive effect through GPR81 at physiologic concentrations of 3-4 mM, while high, sustained levels of lactate can lead to intracellular acidosis and enhance NLRP3-mediated responses (91). Activating the GPR81-lactate axis in antigen-presenting DCs reduces the MHC II expression and decreases the production of cAMP, IL-6, and IL-12, impairing tumor antigen presentation (92). The GPR81-lactate axis in colony macrophages and DCs is crucial for maintaining gut homeostasis and preventing colitis (93). Defective GPR81 weakens lactate signaling and increases MCT expression, suggesting a complex interplay between lactate-dependent and -independent pathways (73). Lactate is the ligand of G-protein-coupled receptor 132 (GPR132) as well. Lactate-GPR132 interaction induces protumoral M2 phenotype to facilitate tumor survival (94, 95). In high-fat diet-fed mice, activation of GPR132 by lactate in macrophages reduces proinflammatory M1 polarization and improves glucose homeostasis in adipocytes via the cAMP/PKA/AMPK signaling pathway (96). As a ligand for GPR81 and GPR132, lactate could regulate various signaling pathways in macrophages and affect immune responses, tumor survival, and glucose homeostasis.

4 Lactate regulates gene expression by affecting post-translational modifications through covalent modifications

The modifications of nucleotide or amino acid residues in DNA and histones can reveal distinct features of the genome, affecting DNA replication, transcription, and repair (97). These modifications include acetylation, methylation, phosphorylation, ubiquitination, acylation, hydroxylation, glycosylation, and others. The histones can be modified on the free N-terminal tails or the globular structures attached to DNA, either enzymatically or non-enzymatically (98). The substrate for acylated modifications often comes from cellular metabolites, such as acetyl-CoA for acetylation. For metabolic intermediates and chromatin changes to interact, enzyme Km/Kd values and substrate concentrations must be comparable (99). Intriguingly, Yingming Zhao’s research team discovers that lactate can modify histone lactylation to regulate gene expression. Lactylation is a post-translational protein modification that involves the addition of lactic acid residue on the lysine of proteins. Histone lactylation has been identified at 26 sites in HeLa cells and 16 sites in bone marrow-derived macrophages (2). Cellular metabolic reprogramming induces an imbalance between glycolysis and the TCA cycle, leading to an increase in histone lactylation. Figure 3 shows that lactate affects cellular activity via histones and non-histone lysine lactylation.

FIGURE 3
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Figure 3 High glycolysis and an extracellular high lactate environment result in increased lactate flux. Lactate serves as a crucial substrate for covalent modification of both histone and non-histone lysine residues, playing a vital role in regulating cellular signaling and gene expression. ARG1, Arginase 1; α-KG, α-Ketoglutarate; Hif-1α, hypoxia-induced factor 1 α; HMGB1, high mobility group protein B1; Ldha, lactate dehydrogenase A; MCT, monocarboxylate transporter 1; METTL3, methyltransferase-like 3; PDGFA, platelet-derived growth factor subunit A; PKM2, pyruvate kinase M2; TCA, tricarboxylic acid cycle; TIM, Tumor-infiltrating myeloid; UPS, ubiquitin-proteasome system; VEGFA, vascular endothelial growth factor-A; YTHDF2, YTH N6-methyladenosine RNA-binding protein 2.

4.1 Lactate modifies histones and regulates epigenetics

In a study led by Yingming Zhao, lipopolysaccharide-activated M1 macrophages were found to enhance glucose metabolism and increase intracellular lactate levels, which led to histone modification and upregulation of genes involved in wound healing, such as Arginase 1 (Arg1) (2). This transformation results in the macrophages acquiring a repair phenotype, known as M2. The findings highlight the unique role of the Warburg effect in affecting epigenetics. Previous research has highlighted the involvement of B-cell adapter (BCAP) in the role of macrophages in intestinal inflammation and tissue repair. Recent discoveries indicate that the decreased production of lactate due to the absence of BCAP leads to reduced histone lactylation, resulting in diminished expression of tissue repair genes and hindering the transition of macrophages to a repair phenotype (100). During early myocardial infarction, monocytes undergo metabolic reprogramming that regulates their anti-inflammatory and pro-angiogenic functions through glycolysis and MCT-mediated histone lactylation (101). These findings underscore the critical function of lactate generation, histone lactylation, and downstream genes in negative feedback regulation, as well as cellular activity and homeostasis maintenance.

Lactate plays important roles in the brain at the molecular and behavioral levels, acting as a vital component in the neuron-astrocyte shuttle for maintaining neuronal activity. Furthermore, lactylation of lysine, which occurs in various brain cells and is regulated by neural excitation and stress, is a widespread phenomenon (102). In Alzheimer’s disease (AD), microglia exhibit pro-inflammatory signaling, accompanied by a metabolic shift from OXPHOS to glycolysis. This metabolic change results in lactate-induced modification of the H4K12 site through a positive feedback loop of glycolysis/epigenetics (H4K12-lactylation)/glycolysis (pyruvate kinase), promoting glycolytic activity and exacerbating microglia dysfunction in AD (103). Through these investigations, further insight into the involvement of lactylation in neuropsychiatric disorders has been gained.

Lactate in the environment can affect lactylation of nearby cells. Environmental lactate can impact nearby cells by promoting lactylation. Metabolic reprogramming of pulmonary myofibroblasts leads to increased glycolysis, intensifying their fibrotic phenotype. Lactate does induce the expression of fibrosis-promoting genes (ARG1, PDGFA, THBS1, and VEGFA) in macrophages through lactylation. Moreover, the fibrotic phenotype is attenuated when the lactylation writer p300 is downregulated (104). Tumor-infiltrating myeloid (TIM) cells promote an immunosuppressive environment in a TME with high lactate levels. This process is facilitated by the primary mediator of m6A alteration, methyltransferase-like 3 (METTL3), which has pro-oncogenic effects in malignancies. Lactate increases METTL3 transcription in TIMs by altering histone H3K18 lactylation and directly enhances the catalytic activity of post-translational METTL3 protein (105). In addition, Histone H3K18 lactylation was discovered to increase the transcription of YTHDF2, an m6A recognition protein, which then favors the degradation of m6A-modified PER1 and TP53 mRNA, speeding up carcinogenesis in ocular melanoma (106). The flexibility of inflammatory Th17 and anti-inflammatory Treg cells to switch under specific circumstances has been discovered, with induction in vitro requiring transforming growth factor (TGF)-β. Previous research has focused on identifying the cytokine combinations responsible for this phenotypic flip, and the underlying mechanism has been debated. Recently, it was demonstrated that lactate inhibits IL-17A production by Th17 cells, while the release of IL-2 by ROS increases Foxp3 expression, leading to elevated lactylation on H3K18 (107). This modification’s involvement in epigenetic alterations appears crucial for T cell phenotypic transition. The identification of histone lactylation has introduced a new level of intricacy in our comprehension of gene expression regulation and chromatin structure.

4.2 Lactate modifies non-histone proteins and regulates protein activity

Lactylation of non-histone proteins has emerged as a pivotal regulatory mechanism across diverse biological processes. Perturbations in lactylation have been identified as pathological factors in many diseases.

During normal multipotent stem cell differentiation, there is a metabolic shift from glycolysis to OXPHOS. This metabolic shift is accompanied by a decrease in lactate levels, which leads to a reduction in lactylation modifications on lysine residues of the ubiquitin-proteasome system (UPS). The reduction in lactylation promotes the assembly of UPS, thereby enhancing its chymotrypsin-like activity. Impaired clearance of mitochondria during mammalian erythropoiesis is one of the mechanisms involved in the development of systemic lupus erythematosus (SLE). This disruption in the clearance process by UPS results in the accumulation of red blood cells (RBCs) containing mitochondria (Mito+ RBCs). These Mito+ RBCs are then phagocytosed by macrophages, which triggers the production of type I interferon (IFN) by macrophages (108). The TGF-β/Smad2 pathway, which mediates endothelial-to-mesenchymal transition, plays a critical role in cardiac fibrosis, and upregulation of this pathway exacerbates fibrosis. Lactylation of Snail1, a transcription factor in the TGF-β pathway, promotes its nuclear localization and enhances its ability to bind to the TGF-β promoter, resulting in increased TGF-β production (109). Lactylation of the K62 site on pyruvate kinase M2 (PKM2) improves kinase activity and helps transform pro-inflammatory macrophages into reparative phenotypes by decreasing PKM2 nuclear translocation and tetramer-to-dimer transition (110).

Treg cells can effectively function in high-lactate environments due to their strong gluconeogenesis and utilization of lactate as a carbon source. Recent research has revealed that lactylated MOESIN at position 72 could increase its affinity for TGF-β receptor I and downstream signaling pathways. This results in elevated levels of TGF-β induced Foxp3 expression and Treg cell amount (111). During sepsis, extracellular lactate is taken up by macrophages via MCT, leading to lactylation of the nuclear protein high mobility group box 1 (HMGB1). This results in the release of HMGB1 from macrophages as exosomes, which increases endothelial permeability due to the protein’s pro-inflammatory properties (112).

Lactylation was identified as a key global regulator in hepatocellular carcinoma (HCC), affecting 9256 non-histone protein sites (113). Interestingly, lactylation preferentially affects enzymes involved in metabolic pathways. Lactylation of K28 in adenylate kinase 2 leads to the inhibition of enzyme function and promotes HCC proliferation and metastasis. Investigating the underlying mechanisms of lactylation’s effects on protein function and its involvement in human disease will be essential to fully comprehending the role of this modification in cellular biology. Further studies are needed to shed light on these aspects.

4.3 Glycolytic Flux and acylation

Short-chain acyl-group-containing molecules can undergo acylation, catalyzed by histone acetyltransferases such as p300 and lysine acetyltransferase 2A (KAT2A, also known as GCN5). Lysine residues of histones can be significantly modified by certain acyl-CoA metabolites, including succinyl-CoA and malonyl-CoA. Succinyl-CoA is produced locally in the nucleus by α-ketoglutarate (α-KG) dehydrogenase, which can then be utilized by KAT2A for histone modification (114). Histone crotonylation (Kcr) results from the modification of histones by the histone acetyltransferase p300 with crotonyl-CoA, which is derived from the crotonate (a short-chain fatty acid) (115). Ketone bodies such as butyrate and β-OHB induce the production of histone lysine butyrylation (Kbu) (116) and β-hydroxybutyrylation (Kbhb) (117), respectively. Kbu and Kcr compete with acetylation for histone-binding sites (116, 118).

Changes in glycolytic flux impact the extent of histone pan-acetylation and show variation at specific modification sites. Notably, certain residues demonstrate no significant histone acetylation changes upon shifts in glycolytic flux, while approximately 40% of acetylation sites display quantitatively altered levels in response to changes in glycolysis. These modified sites exhibit a correlation with both acetyl-CoA levels and acetyl-CoA/CoA ratios (119). All histone propionylation (Kpr), butyrylation (Kbu) and 2-hydroxyisobutyrylation (Khib) demonstrated quantifiable changes in response to shifts in glycolytic flux, across all residue levels (119). Variations in glycolytic flux can modify the extent of histone acylation modifications, with the fluctuating level of glycolytic metabolites exerting a significant impact. Modifications near the globular structural domains of histones are more resistant to changes in glycolysis, while modifications in the histone tails appear to be more sensitive. These observations imply that the proximity to the metabolic environment may influence the viability of specific histone marks (119). Given the interdependent nature of lysine acylation, metabolic pathways, and their products, the kinetics of lactylation and acetylation can both show variable changes in response to glycolytic flux. Furthermore, these modifications may undergo alterations in either the same or opposite directions (2). Lactylation at specific sites serves as a marker for active promoters and exhibits a strong correlation with additional active modifications, including H3K27ac and H3K4me3 (120). Various acylations can elicit synergistic effects on non-histone proteins. For example, the lactylation and acetylation of HMGB1 synergistically increase endothelial permeability and the modified HMGB1 is released from macrophages via exosomes (112).

Efficient allocation of limited resources is essential for organisms to optimize growth, reproduction, and survival in their respective environments, with metabolism serving as a fundamental biological function underlying these processes. In 1958, Otto Heinrich Warburg pointed out that the switch to glycolysis was not only characteristic of tumor cell growth but also a defining feature of immune cell activation (121). Despite this insight, it was not until 2011 that the concept of immunometabolism emerged, linking the two disciplines of immunology and metabolism (122). Metabolic adaptation is now recognized as a critical initial step in immune responses, where immune cells switch between different metabolic modes based on environmental signals to perform their functions. Research on metabolic reprogramming has evolved from studying the Warburg effect to investigating fundamental energy substrates such as glucose, fatty acids, and amino acids. These substrates serve as both cofactors and substrates for epigenome-modifying enzymes, creating a link between metabolic and chromatin states. The interplay between metabolism, epigenetics, and post-transcriptional regulation is a promising yet underexplored area of research with potential to uncover mechanisms of cellular life processes.

Author contributions

HW wrote the manuscript and draw the illustrations. HW and YZ contributed to the literature search. HH and YZ designed and supervised the study. All authors contributed to the article and approved the submitted version.

Funding

This research was supported by the National Key Research and Development Program of China (grant nos. 2022YFA1103500).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

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Keywords: metabolic reprogramming, epigenetics, glycolysis, lactate, lactylation

Citation: Wu H, Huang H and Zhao Y (2023) Interplay between metabolic reprogramming and post-translational modifications: from glycolysis to lactylation. Front. Immunol. 14:1211221. doi: 10.3389/fimmu.2023.1211221

Received: 24 April 2023; Accepted: 19 June 2023;
Published: 29 June 2023.

Edited by:

Noah Isakov, Ben-Gurion University of the Negev, Israel

Reviewed by:

Vinay Bulusu, Indian Institute of Science Education and Research Berhampur (IISER), India
Martin Böttcher, Otto-von-Guericke University Magdeburg, Germany

Copyright © 2023 Wu, Huang and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: He Huang, aHVhbmdoZUB6anUuZWR1LmNu; Yanmin Zhao, eWFubWluemhhb0B6anUuZWR1LmNu

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