Transcriptomic Analysis of Skin Biopsies in Prurigo Nodularis Patients: With and Without Atopic Dermatitis

So Yeon Lee¹Ji Young Um¹Han Bi Kim¹Hyun-Woo Yang²Bo Young Chung¹Chun Wook Park¹Hye one Kim^1*

• ¹Hallym University Kangnam Sacred Heart Hospital, Seoul, Republic of Korea
• ²Upper Airway Chronic Inflammatory Diseases Laboratory, Korea University College of Medicine, seoul, Republic of Korea

Abstract Background: Nodular dermatitis (PN) is a severely itchy chronic skin disease with symmetrically distributed nodules, often linked to an atopic background in some patients. However, the pathogenesis of PN with atopic dermatitis remains unclear. Objective: The objective of this study is to compare the transcriptomes from skin biopsies of prurigo patients with and without atopic dermatitis, aiming to identify unique gene expression patterns and gain insights into the molecular mechanisms underlying Atopic dermatitis Prurigo (ADP) and Non-Atopic dermatitis Prurigo (NADP). Method: We conducted transcriptome analysis to compare gene expression between normal controls and atopic dermatitis patients, identifying DEGs and performing KEGG and GO analyses, along with correlations between disease severity and itch NRS. Results: We performed transcriptome profiling on 5 patients with ADP, 6 patients with NADP, and 6 healthy controls. Gene expression analysis revealed significant differences in inflammatory cytokines, suggesting that cytokine-mediated pathways play an important role in the pathogenesis of ADP.