ORIGINAL RESEARCH article
Front. Immunol.
Sec. Alloimmunity and Transplantation
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1576815
This article is part of the Research TopicAntibody-Mediated Rejection After Solid Organ TransplantationView all 13 articles
Impact of human leukocyte antigen mismatch between donor-recipient on acute rejection in liver transplantation using next-generation sequencing: A single-center study
Provisionally accepted
- 1Ningbo Medical Centre Lihuili Hospital, Ningbo, China
- 2Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China
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The effect of human leukocyte antigen (HLA) mismatch on acute rejection (AR) in liver transplantation (LT) is controversial. This study aimed to investigate the effect of donorrecipient mismatch at HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1 and -DPB1 loci on AR in LT.Methods: 92 patients who underwent LT were selected for investigation from January 1, 2018 to June 30, 2024, and the donors of these patients were also from the same hospital. All donor and recipient specimens were genotyped via next-generation sequencing (NGS) for 11 HLA loci. The patients were divided into AR and non-AR groups according to whether AR occurred after LT.Results: A total of 12 cases (13.04%) occurred AR after LT. The proportion of chronic HBV was lower in the AR group than that in the non-AR group (P<0.05), while the proportion of split LT and mortality within one year after transplantation were higher in the AR group than in the non-AR group (P<0.05). Compared with the non-AR group, the AR group had a significantly higher proportion of high-mismatch DQB1 (2 vs. 0-1) and DRB1+DQB1 (4 vs. 0-3) (P<0.05) at allele level, and other mismatches of a single locus and different combinations of 11 HLA loci were no significant differences between the two groups (P>0.05). However, neither high-mismatch DQB1 nor highmismatch DRB1+DQB1 at allele level was an independent risk factor for AR after adjustment for chronic HBV infection, operative procedures of LT, and immunosuppressive regimen using bootstrapping (OR: 0.203, 95% CI: 0.000-1.300, P=0.067; OR: 0.404, 95% CI: 0.000-2.625, P=0.172, respectively). Conclusion: In this preliminary study, no correlation between HLA mismatch at allele level and post-transplant AR episodes was found.
Keywords: Liver Transplantation, acute rejection, human leukocyte antigen, mismatch, nextgeneration sequencing
Received: 14 Feb 2025; Accepted: 28 Apr 2025.
Copyright: © 2025 Lu, Lu, He, Chen and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Wei Chen, Ningbo Medical Centre Lihuili Hospital, Ningbo, China
Faming Zhu, Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China
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