ORIGINAL RESEARCH article
Front. Immunol.
Sec. Autoimmune and Autoinflammatory Disorders : Autoimmune Disorders
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1577206
Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel
Provisionally accepted- 1Canberra Clinical Phenomics Service, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 2Phenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 3Department of Medicine, University of Cambridge, Cambridge, United Kingdom, Cambridge, United Kingdom
- 4ANU Centre for Therapeutic Discovery, John Curtin School of Medical Research, Australian Phenomics Facility, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 5Cytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 6Division of Genome Science and Cancer, John Curtin School of Medical Research, College of Health and Medicine, Australian National University, Acton, Australian Capital Territory, Australia
- 7Division of Immunology and Infectious Disease, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 8Department of Renal Medicine, The Canberra Hospital, Canberra, ACT, Australia, Canberra, Australia
- 9National Centre for Epidemiology and Population Health, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 10School of Medicine and Psychology, The Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 11Division of Genome Science and Cancer, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia, Canberra, Australia
- 12South Australian Health and Medical Research Institute, Adelaide, SA, Australia, Adelaide, Australia
- 13Department of Immunology, and Department of Immunopathology, ACT Pathology, Canberra Health Services, Canberra, ACT, Australia, Canberra, Australia
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Lymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. The purpose of our study was to measure many lymphocyte populations from a single sample, with the long-term aim of validating our assay for diagnostic use in the Australian regulatory environment. We designed and optimised a novel 30-colour lymphocyte immunophenotyping panel tailored for use on a 3-laser (V-B-R) spectral flow cytometer. This panel measures over 50 lymphocyte populations. In this report we present data derived from 148 healthy individuals. This lays the groundwork for future clinical application of spectral flow cytometry tests and offers a more comprehensive approach to lymphocyte analysis with future implications for disease diagnosis and monitoring.
Keywords: Spectral flow cytometry, lymphocyte, PBMC, Immunophenotyping, Autoimmunity, T cell, B cell
Received: 15 Feb 2025; Accepted: 30 Jun 2025.
Copyright: © 2025 Davies, Kwong, Yu, Diamand, Li, Kannitha, Ali, Amjadipour, Padarin, Devoy, Vohra, Miraghazadeh, Jiang, Bruestle, Cherbuin, Nolan, Cook, Gardiner, Read, McNaughton and Randall. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Katrina Louise Randall, Canberra Clinical Phenomics Service, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia, Canberra, Australia
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