ORIGINAL RESEARCH article
Front. Immunol.
Sec. Parasite Immunology
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1585883
Role of the CX3CL1/CX3CR1 axis in iron metabolism and immune regulation during acute Trypanosoma cruzi infection
Provisionally accepted- 1Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil
- 2Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
- 3School of Medicine, University of California San Diego, La Jolla, California, United States
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During Trypanosoma cruzi infection, the immune system activates a robust inflammatory response, involving cytokines and chemokines like IFN-γ, TNF, IL-6, IL-1β, CCL2, and CCL5, to control parasite replication. The CX3CL1 chemokine and its receptor, CX3CR1, have been implicated in amplifying inflammation through pathways like NF-κB, MAPKs, STATs, TLRs, and NLRs, contributing to tissue damage. This study evaluated the effects of blocking CX3CR1 with the allosteric antagonist AZD8797 in a murine model of acute T. cruzi infection. Male C57BL/6 mice were infected with 10³ trypomastigote forms of T. cruzi (Y strain) and received AZD8797 (10 mg/kg) intraperitoneally for 10 days. On the 10th day, animals were euthanized and heart, skeletal muscle, and liver tissues were collected for CX3CL1 protein expression, biomarkers (IL-1β, IL-4, IL-6, IL-10, IL-15, IL-17, IFN-γ, TNF, and CCL2) quantified by Cytometric Bead Array and Enzyme Immunoassay. Treatment reduced spleen mass and cardiac levels of CCL2 and IL-15, with an increase of IL-4. Conversely, in skeletal muscle, TNF, IL-6, and IL-10 increased, while IL-15 decreased. Liver tissue showed reduced IL-15, IL-6, and IL-1β levels, alongside lowered plasma hepcidin and ferritin concentrations. These findings highlight CX3CL1's site-specific role in modulating inflammation and iron metabolism during acute T. cruzi infection, suggesting its potential as a therapeutic target for infection management and disease prognosis.
Keywords: Inflammation, Trypanosoma cruzi, cardiac disease, CX3CR1, CX3CL1
Received: 01 Mar 2025; Accepted: 31 Jul 2025.
Copyright: © 2025 Pio, Menezes, Louise, Costa, Oliveira Malta, Carlos¹, Paula-Gomes, Oliveira Perucci and Talvani. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Andre Talvani, Universidade Federal de Ouro Preto, Ouro Preto, 35400-000, Minas Gerais, Brazil
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