ORIGINAL RESEARCH article

Front. Immunol.

Sec. Viral Immunology

Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1599468

This article is part of the Research TopicAnimal-borne viral disease: Pathogenesis, Innate immunity, Acquired immunity, and Novel vaccine developmentView all 12 articles

Immunopathological Features of Highly Pathogenic Korean Lineage B (LKB) PRRSV-2: Insights into Virulence Indicators and Host Immune Responses

Provisionally accepted
Gyeong-Seo  ParkGyeong-Seo Park1,2Seung-Chai  KimSeung-Chai Kim1Hwan-Ju  KimHwan-Ju Kim1Chang-Gi  JeongChang-Gi Jeong1,3Sang-Chul  KangSang-Chul Kang4Go-Eun  ShinGo-Eun Shin5Seoung-Hee  KimSeoung-Hee Kim5Hye-Young  JeongHye-Young Jeong5Kyoung-Ki  LeeKyoung-Ki Lee5Sang-Myeong  LeeSang-Myeong Lee6Won-Il  KimWon-Il Kim1*
  • 1Collenge of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea
  • 2Vaccine Lab, WOOGENE B&G Co., LTD., Seoul, Republic of Korea
  • 3Biosafety Research Institute, Jeonbuk National University, Iksan, Republic of Korea
  • 4Animal Clinical Evaluation Center, Optipharm Inc, Cheongju, Republic of Korea
  • 5Animal and Plant Quarantine Agency (South Korea), Gimcheon-si, North Gyeongsang, Republic of Korea
  • 6College of Veterinary Medicine, Chungbuk National University, Cheongju, North Chungcheong, Republic of Korea

The final, formatted version of the article will be published soon.

Porcine reproductive and respiratory syndrome virus (PRRSV) remains one of the most economically devastating pathogens in swine, primarily due to its extensive genetic diversity and lineage-dependent pathogenicity. Despite widespread vaccination, distinct PRRSV-2 lineages continue to circulate in Korea. This study aimed to elucidate the immunopathological features of two Korean-specific Lineage B (LKB) strains, GGYC45 and PJ10, compared with a vaccine-like L5 strain, M8. Thirty, 4-week-old piglets were divided into M8-, GGYC45-, PJ10-infected groups, and control. After acclimatization, pigs were intramuscularly inoculated with PRRSV-2 strains. Pigs were monitored, and blood and nasal swabs were collected. At 12-and 28 days post-infection (dpi), pigs were euthanized for histopathological analysis and tissue collection. Histopathological evaluations were conducted on lung and brain tissues. Bronchoalveolar lavage (BAL) cells and lung tissues were analyzed for immune responses, including flow cytometry (FACS), cytokine expression, viral load, and expression of immune checkpoint molecules. Both LKB strains (GGYC45 and PJ10) observed moderate to severe clinical symptoms. Notably, PJ10-infected pigs exhibited high mortality accompanied by significantly (p < 0.05) low average daily weight gain (ADWG), high temperatures, and high levels of viremia and viral loads in various tissues. Immunopathological analysis showed severe respiratory and neurological lesions in PJ10-infected pigs. PJ10 destroyed over 90% of residential alveolar macrophages and increased infiltrated monocyte-derived cells and T lymphocytes in the lungs up to 12 dpi. Pigs infected with the GGYC45 strain exhibited a relatively lower virulence profile than those infected with the PJ10 strain; however, GGYC45 induced moderate pathogenicity in pigs. Regardless of the lineages or genotypes, pigs infected with PRRSV-2 increased immune checkpoint molecule expression, such as PD1, PDL1, CTLA4, IDO1, and LAG3 in BAL cells, resulting in insufficient T cell activation. These results highlight the differential virulence and immunomodulatory profiles of genetically distinct PRRSV-2 strains circulating in Korea. The heightened immune checkpoint expression, particularly in PJ10-infected pigs, underscores a potential mechanism of PRRSV-induced immune suppression and viral persistence. This study provides critical insights into PRRSV pathogenesis and host-virus interactions and supports the need for lineage-adapted control strategies that account for both the genetic heterogeneity of PRRSV and immune evasion mechanisms.

Keywords: Porcine Reproductive and Respiratory Syndrome Virus, Korean Lineage, pathogenicity, Immune characteristics, host immunity, immune checkpoint molecules

Received: 25 Mar 2025; Accepted: 26 May 2025.

Copyright: © 2025 Park, Kim, Kim, Jeong, Kang, Shin, Kim, Jeong, Lee, Lee and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Won-Il Kim, Collenge of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea

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