BRIEF RESEARCH REPORT article
Front. Immunol.
Sec. Inflammation
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1606408
This article is part of the Research TopicExploring Sulfur Compounds' Role in Inflammation and Therapeutic PotentialView all articles
Sulphur compounds navigate redox processes, leukotriene synthesis and ω-hydroxylation of leukotriene B4 at neutrophil interaction with bacteria Salmonella typhimurium: the way to manipulate neutrophil swarming
Provisionally accepted
Ekaterina Golenkina1
Sofia V. Navarnova1Galina M. Viryasova1
Svetlana Galkina1Tatjana V. Gaponova2Yulia M. Romanova3
Galina F. Sud’ina1*- 1Lomonosov Moscow State University, Moscow, Russia
- 2National Research Center for Hematology, Russia Federation Ministry of Public Health, Moscow, Moscow Oblast, Russia
- 3N.F. Gamalei Federal Research Centre for Epidemiology and Microbiology, Ministry of Health Russian Federation, Moscow, Moscow Oblast, Russia
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Neutrophils are the first immune cells recruited by invading pathogens. In interaction with bacteria, neutrophils synthesize leukotriene B4, a potent chemoattractant that, in conjunction with the primary bacterial chemoattractant fMLP, stimulates the formation of neutrophil clusters surrounding pathogens. Hydrogen sulfide (H2S) plays a critical role in the regulation of host-bacteria interaction; bacteria are known to use hydrogen sulfide in response to host-induced oxidative stress. The purpose of this study was to investigate the regulatory role of H2S in neutrophil cellular responses in an experimental model of neutrophils interaction with Salmonella typhimurium. In neutrophil interaction with bacteria, the application of H2S donor (NaSH) increased leukotriene synthesis stimulated by the peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) in neutrophils after preincubation with bacteria. NaSH significantly suppressed reactive oxygen species (ROS) formation in neutrophils. When we used PMA in cells pre-treatment before adding fMLP, we observed decreased leukotriene synthesis and increased ROS formation in cells. Not producing ROS disulfide stress induced by diamide, in combination with NaSH, synergistically increased fMLP-induced leukotriene synthesis in neutrophil interaction with bacteria Salmonella typhimurium. The data obtained demonstrate that not producing ROS disulfide stress increases LT synthesis in the presence of H2S-producing compounds.
Keywords: Neutrophil, Salmonella typhimurium, Leukotriene B4, Reactive Oxygen Species, Glutathione, neutrophil swarming
Received: 05 Apr 2025; Accepted: 29 Sep 2025.
Copyright: © 2025 Golenkina, Navarnova, Viryasova, Galkina, Gaponova, Romanova and Sud’ina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Galina F. Sud’ina, sudina@genebee.msu.ru
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