ORIGINAL RESEARCH article

Front. Immunol.

Sec. Inflammation

Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1617146

This article is part of the Research TopicExploring Immunological Factors in Inner Ear Disorders: Allergy and InflammationView all articles

Organ of Corti Macrophages: A Distinct Group of Cochlear Macrophages with Potential Roles in Supporting Cell Degeneration and Survival

Provisionally accepted
  • 1University at Buffalo, Buffalo, New York, United States
  • 2University of the Pacific, San Francisco, United States
  • 3The University of Texas at Dallas, Richardson, United States

The final, formatted version of the article will be published soon.

Macrophages are the primary immune cells in the cochlea, essential for maintaining cochlear homeostasis and orchestrating inflammatory responses to pathological events. Although these cells have been found in various parts of the cochlea, their presence in the organ of Corti, a critical structure for acoustic sensing, remains poorly understood. The present study was designed to examine macrophage responses to ototoxic drug-induced cochlear damage and age-related cochlear degeneration, with a particular focus on the pathological conditions that trigger macrophage recruitment into the organ of Corti. We used a model of ototoxicity induced by cyclodextrin, a cyclic oligosaccharide known for its ability to induce rapid sensory cell damage at high doses. Cochlear tissues were collected for macrophage assessment. Multiple protein markers, including CD45, Iba1, galectin-3, CD68, myosin 7α, and IFIT3, were used to label sensory cells, macrophages, and supporting cells in cochlear sensory epithelia. Consistent with previous reports, our study confirms that macrophages are absent from the organ of Corti in mouse cochleae under normal conditions and during the acute phase of cochlear damage. However, macrophages enter the organ of Corti during the chronic phase of cochlear pathogenesis. These macrophages exhibit an activated state and display a distinct functional profile compared to macrophages outside the organ of Corti. Importantly, we demonstrate that the organ of Corti macrophage activity is not directly related to the process of sensory cell degradation. Instead, their activity is associated with supporting cell pathogenesis. Moreover, our study shows that the organ of Corti macrophages are also present in aging mouse cochleae. Collectively, our findings reveal the conditions that lead to macrophage recruitment into the organ of Corti and their involvement in supporting cell survival and degeneration. These findings provide valuable insights for future strategies focused on modulating macrophage activity to reduce tissue damage and promote repair in the cochlea following injury.

Keywords: macrophage, Cochlea, Organ of Corti, supporting cells, Inflammation, galectin-3, ototoxicity, Aging

Received: 23 Apr 2025; Accepted: 06 Jun 2025.

Copyright: © 2025 Ye, Zhang, Ding, Chen, Adler, Sharaf and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Bohua Hu, The University of Texas at Dallas, Richardson, United States

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