ORIGINAL RESEARCH article
Front. Immunol.
Sec. Parasite Immunology
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1617570
This article is part of the Research TopicImmune-Related Mechanisms in Apicomplexan and Trypanosomatid ParasitesView all articles
NcROP2 deletion reduces Neospora caninum virulence by altering parasite stage differentiation and hijacking host immune response
Provisionally accepted
Rafael Amieva1
Montserrat Coronado1
Jessica Powell2
David Arranz-Solís1
Musa A. Hassan2
Esther Collantes-Fernandez1*
Luis-Miguel Ortega-Mora1
Pilar Horcajo1- 1SALUVET Group, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain
- 2Division of Infection and Immunity, Roslin Institute, University of Edinburgh, Edinburgh, Scotland, United Kingdom
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Neospora caninum is an apicomplexan parasite responsible for bovine neosporosis, a major cause of abortion in cattle worldwide. N. caninum rhoptry protein 2 (NcROP2) has been identified as an essential factor in host cell invasion and parasitophorous vacuole formation, making it a potential target for disease control strategies. In this study, we generated NcRop2 knockout (NcΔROP2) mutants using CRISPR/Cas9 technology to assess their role in parasite virulence. In a pregnant mouse model, NcΔROP2 parasites exhibited reduced virulence, as indicated by increased neonatal survival rates and lower parasite burden in the brain and attenuated clinical signs in the dams compared to the wild-type (Nc-Spain7) parental strain.Additionally, the NcΔROP2 mutants exhibited impaired proliferation and significantly induced the expression of interferon-stimulated genes in bovine monocyte-derived macrophages infected in vitro for 60 hours. Transcriptomic analysis further revealed a shift in parasite gene expression, with an upregulation of stress-related and bradyzoite markers. Functional assays confirmed that NcΔROP2 parasites were less susceptible to IFN-γ-mediated inhibition and displayed an enhanced ability to convert to the semi-dormant bradyzoite stage. These findings highlight NcROP2 as a key virulence factor involved in immune evasion and parasite proliferation, providing new insights into N. caninum infection pathogenesis and potential avenues for vaccine development.
Keywords: Neospora caninum, virulence factor, NcROP2, CRISPR/Cas9, BALB/c, bovine macrophages, Transcriptome
Received: 24 Apr 2025; Accepted: 21 Jul 2025.
Copyright: © 2025 Amieva, Coronado, Powell, Arranz-Solís, Hassan, Collantes-Fernandez, Ortega-Mora and Horcajo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Esther Collantes-Fernandez, SALUVET Group, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain
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