ORIGINAL RESEARCH article
Front. Immunol.
Sec. Autoimmune and Autoinflammatory Disorders : Autoimmune Disorders
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1636162
Monoclonal Immunoglobulins on Red Blood Cells: A Potential Supplementary Diagnostic Indicator for Monoclonal Gammopathies
Provisionally accepted
Benxia Bing
Xiaowei Yang
Liang XuChenghua WangShiqing ChengYun LiuQiuhan WangBing ZhaoChunjuan Zhai
Rong Wang*Jing Sun*- Shandong Provincial Hospital Affiliated to Shandong First Medical University, jinan, China
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Introduction: Red blood cells (RBCs) had the capability to bind and transport a variety of substances, the aim of this study is to investigate the potential of circulating RBCs as carriers of monoclonal immunoglobulins (M-proteins).Methods: Patients who were newly diagnosed with monoclonal gammopathy in the Nephrology Department of Shandong Provincial Hospital affiliated to Shandong First Medical University from April 2023 to December 2024 were included in this study. Western blots were performed on RBC membrane proteins using primary antibodies against human IgG, IgM, IgA, kappa, lambda.Results: 49 patients with monoclonal gammopathy were enrolled in this study. Substantial increases in the amounts of erythrocyte-bound immunoglobulins, characterized by monoclonal properties were observed. Among the 49 patients, 45 (91.8%) had M-proteins detected on RBCs, 38 (77.6%) had abnormal serum capillary electrophoresis and immunosubtraction (CE/IS) results, 36 (75.3%) had abnormal serum free light-chain (sFLC) ratios, and 32 (65.3%) had abnormal serum total light chains (sTLCs) ratios. 53.1% (26/49) of patients with monoclonal gammopathy had concordant results between erythrocyte Western blot and CE/IS. 22.4% (11/49) of patients had negative serum CE/IS results but extrinsic monoclonal immunoprotein bands on RBCs, which were consistent with the findings from sFLC, bone marrow flow cytometry, or renal histopathology. 16.3% (8/49) of patients had abnormal but inconsistent results between serum CE/IS and erythrocyte electrophoresis. 4 (8.2%) patients had no or polyclonal immunoprotein bands on RBCs.Monoclonal immunoglobulins were presented on erythrocytes, which may serve as a supplementary diagnostic indicator for the detection of M-proteins.
Keywords: monoclonal gammopathy, Monoclonal immunoglobulins, capillary electrophoresis and immunosubtraction, red blood cells, western blot
Received: 03 Jun 2025; Accepted: 25 Aug 2025.
Copyright: © 2025 Bing, Yang, Xu, Wang, Cheng, Liu, Wang, Zhao, Zhai, Wang and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Rong Wang, Shandong Provincial Hospital Affiliated to Shandong First Medical University, jinan, China
Jing Sun, Shandong Provincial Hospital Affiliated to Shandong First Medical University, jinan, China
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