ORIGINAL RESEARCH article
Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1638792
Integrative Bulk and Single-Cell Transcriptomic Analysis Identifies a Migrasome-Associated lncRNA Signature Predictive of Prognosis and Immune Landscape in Clear Cell Renal Cell Carcinoma
Provisionally accepted
- Second Hospital of Tianjin Medical University, Tianjin, China
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Clear cell renal cell carcinoma (ccRCC), the predominant subtype of renal cell carcinoma, has a poor prognosis due to high recurrence and metastasis rates. Migrasomes, organelles involved in intercellular communication, and long non-coding RNAs (lncRNAs) play critical roles in cancer progression. This study identified migrasome-associated lncRNAs using TCGA data to construct a prognostic signature that stratified patients into high-and low-risk groups with significant survival differences. Functional analyses revealed distinct immune and metabolic pathways between groups, with high-risk patients exhibiting elevated tumor mutation burden and immune evasion potential.Drug sensitivity analysis identified differential therapeutic responses, suggesting clinical utility in personalized treatment. Single-cell transcriptomic analysis revealed pronounced cellular heterogeneity and tumor microenvironmental features associated with the prognostic signature. Highrisk cells were predominantly enriched in tumor epithelial clusters and exhibited distinct patterns of intercellular communication. Furthermore, the signature genes displayed marked cell type-specific expression, suggesting their potential involvement in angiogenesis, immune regulation, and tumor progression. Experimental validation demonstrated that knockdown of FOXD2-AS1, an oncogenic lncRNA from the signature, suppressed ccRCC cell proliferation and migration, highlighting its role in tumor progression. These findings underscore the prognostic and therapeutic relevance of migrasome-associated lncRNAs in ccRCC.
Keywords: Clear cell renal cell carcinoma, Migrasome, Prognostic signature, Tumor mutation burden, Tumor immune microenvironment, drug sensitivity, single-cell RNA sequencing
Received: 31 May 2025; Accepted: 31 Jul 2025.
Copyright: © 2025 Shen, Wang, Zhang, Chen, Liu, Tian and Shang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Liwei Liu, Second Hospital of Tianjin Medical University, Tianjin, China
Jing Tian, Second Hospital of Tianjin Medical University, Tianjin, China
Zhiqun Shang, Second Hospital of Tianjin Medical University, Tianjin, China
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