ORIGINAL RESEARCH article
Front. Immunol.
Sec. Autoimmune and Autoinflammatory Disorders : Autoimmune Disorders
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1647466
Lactobacillus plantarum-Derived Cytoplasmic Membrane Vesicles as Novel Anti-Inflammatory Nanotherapeutics for Psoriasis Management
Provisionally accepted
- 1Key Laboratory for Precision Diagnosis and Treatment of Pediatric Digestive System Diseases, Shenzhen Children's Hospital, Shenzhen, China
- 2School of Pharmaceuticals Sciences (Shenzhen), Sun Yat-sen University, Guangzhou, China
- 3College of Life Science, Northwest A&F University, Xianyang, Shaanxi, China
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Current research emphasizes the gut-skin axis in inflammatory skin disorders, particularly the therapeutic potential of probiotics, though their precise mechanisms and bioactive components remain elusive. In this study, through analysis of microbiome dysbiosis associated with various inflammatory skin diseases including psoriasis, we identified Lactobacillus plantarum (Lp) as a key depleted species. Systematic screening of four Lp-derived bioactive fractions — cytoplasmic membrane vesicles (CMVs), bacterial lysate supernatant (BL-S), bacterial lysate precipitate (BL-P), and cell-free fermentation supernatant (CFS) — revealed that CMVs and BL-S shared core therapeutic properties, including anti-inflammatory capacity, reactive oxygen species (ROS) scavenging activity, and inhibition of hyperproliferative keratinocytes. Notably, CMVs exhibited unique immunomodulatory functions by attenuating the polarization of pro-inflammatory macrophages. Metabolomic profiling identified anandamide (AEA), an endocannabinoid system modulator, as the predominant bioactive component within CMVs, exerting anti-inflammatory, antioxidative, and macrophage polarization regulatory effects. Finally, in imiquimod-induced psoriatic mice, CMVs administration significantly alleviated psoriasis symptoms due to effective reduction of hyperkeratosis, regulation of inflammation and immunosuppression. This study not only establishes Lp-derived CMVs as a potent non-viable probiotic therapy for psoriasis but also pioneers a functional screening paradigm for identifying microbial nanovesicles with precision microbiome therapy.
Keywords: Lactobacillus plantarum, bacterial membrane vesicles, Psoriasis, anandamide, precision microbiome therapy
Received: 18 Jun 2025; Accepted: 23 Sep 2025.
Copyright: © 2025 Xie, Lv, Su, Li, Xu, Chen, Cheng and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongbo Chen, chenhb7@mail.sysu.edu.cn
Fang Cheng, chengf9@mail.sysu.edu.cn
Dongling Dai, daidong3529@sina.com
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