ORIGINAL RESEARCH article
Front. Immunol.
Sec. Molecular Innate Immunity
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1654180
This article is part of the Research TopicDevelopment of Diagnostic and Therapeutic Biomarkers for Tumors and Inflammation Based on Multi-omics Approaches Including Transcriptomics, Proteomics, and MetabolomicsView all 4 articles
Prognostic Model of Ubiquitination-Related Genes in Ovarian Cancer Based on Transcriptomic Analysis and Experimental Validation
Provisionally accepted
- Guizhou Medical University, Guiyang, China
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Objective: Ubiquitination plays a crucial role in the malignant progression of ovarian cancer. With the advent of proteolysis-targeting chimeras (PROTACs) targeting ubiquitin enzymes, precision therapies are now possible. Therefore, it is imperative to ascertain the prognostic significance of ubiquitination-related genes in ovarian cancer. Methods: A prognostic model based on ubiquitination-related genes was developed using data from TCGA and GTEx databases. Performance was assessed via Kaplan-Meier, ROC curves, and Cox regression; a nomogram was created. The model's stability was checked using training and test sets. FBXO45 was also experimentally validated in ovarian cancer. Results: The model, based on 17 genes related to ubiquitination (e.g., TRAF4, UBE2L3, WDR77, FBXO45, SKIP2, UBE2L6, FBXL14), showed high performance (1-year AUC = 0.703, 3-year AUC = 0.704, 5-year AUC = 0.705). The high-risk group had significantly lower overall survival (P < 0.05). Immune analysis showed higher levels of CD8+ T (P < 0.05), M1 (P < 0.01) and follicular (P < 0.05) cells in the low-risk group. High-risk patients had more mutations in MUC17 and LRRK2, while low-risk patients had more RYR2 mutations. FBXO45 is a key E3 ubiquitin ligase in ovarian cancer, promoting growth, spread and migration via the Wnt/β-catenin pathway. Conclusion: Ubiquitination-related markers provide reliable prognostic insights and reflect the immune microenvironment in ovarian cancer, offering a basis for clinical targeting strategies.
Keywords: ovarian cancer, Ubiquitination, Prognostic model, Immunotherapy, FBXO45, Wnt/β-catenin signaling pathway
Received: 26 Jun 2025; Accepted: 13 Aug 2025.
Copyright: © 2025 Xiaojing, Zhao, Li, Huang, Zhong, Luo, Zhang, Li and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Qinshan Li, Guizhou Medical University, Guiyang, China
Mengxing Li, Guizhou Medical University, Guiyang, China
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