ORIGINAL RESEARCH article
Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1654655
Increased IgG4 expression with tertiary lymphoid structures of esophageal cancer and implications for prognosis
Provisionally accepted
- 1Shantou University Medical College, Shantou, China
- 2The First People's Hospital of Yunnan Province, Kunming, China
- 3College of Medicine, Shantou University, Shantou, China
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Tertiary lymphoid structures (TLS) have been observed in various tumors, including esophageal squamous cell carcinoma (ESCC), where they function as active centers of humoral immunity within the tumor microenvironment, facilitating the production of distinct immunoglobulin (Ig) subtypes with diverse effects. This study aimed to investigate the expression of TLS-associated Ig in ESCC and its prognostic significance. A retrospective analysis was conducted on 109 ESCC cases. TLS presence was evaluated using immunohistochemistry, and the expression of Ig-positive cells was assessed.Additionally, gene expression profiles related to TLS were analyzed with gene chip data from 274 ESCC cases obtained from the GEO database. Among the 109 ESCC cases, 69 (63.3%) exhibited TLS, while 40 (36.7%) did not. Patients with TLS demonstrated significantly better prognoses compared to those without TLS (P < 0.001). ESCC tumor tissues with TLS exhibited higher infiltration of Ig-positive immune cells. Among the variables examined, the differential expression of IgG4 was the most pronounced (P = 0.04). Furthermore, ESCC cases exhibiting TLS with elevated IgG4 expression demonstrated a poorer prognosis compared to those with TLS and reduced IgG4 expression (P = 0.036).The presence of TLS in general and low IgG4 expression in TLS were associated with improved survivals in patients who underwent post-surgery chemotherapy (p < 0.001 and p = 0.001, respectively). Analysis of the GEO dataset indicated that predominant IgG4 expression within TLS was associated with a reduced number of activated CD8+ T cells and an increased presence of CD4+ T helper cells and dendritic cells. The expression of TLS in ESCC is linked to a more favorable prognosis, whereas TLS with increased IgG4 expression correlates with a less favorable prognosis. The differential expression of immunoglobulins within TLS can modulate its function within the tumor microenvironment.
Keywords: esophageal cancer, Immunoglobulin G4, tertiary lymphoid structure, Tumor Microenvironment, prognosis
Received: 26 Jun 2025; Accepted: 21 Aug 2025.
Copyright: © 2025 Su, Chen, He, Ma, Huang, Zhu, Wang and Gu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hui Wang, The First People's Hospital of Yunnan Province, Kunming, China
Jiang Gu, College of Medicine, Shantou University, Shantou, China
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